The Protein-Bound Carbohydrates of Seromucoid from Normal Human Serum

Abstract

The neutral sugars and hcxosamines in the seromucoid fraction of normal human serum were identified and determined simultaneously by gas-liquid chromatography of their aldononitrilc acctates. Besidcs the sialic acid, normal seromucoid contained fucose, arabinose, mannosc, glucose, galactose, glucosamine and galactosamine. The presence of arabinose and glucose, not previously reported in seromucoid, äs well äs the other known carbohydrate components determined by GLC were confirmed from their fragmentation patterns in the electron impact mass spectra and chemical ionization spcctra. Die Protein-gebundenen Kohlenhydrate von Seromucoid aus normalem Serum des Menschen Zusammenfassung: Die Neutralzucker und Hexosamine in der Seromucoidfraktion aus normalen Serum des Menschen wurden durch Gaschromatographie ihrer Aldononitrilacetate identifiziert und gleichzeitig bestimmt. Neben Neuraminsäure enthielt normales Seromucoid Fucose, Arabinose, Mannose, Glucose, Galaktose, Glucosamin und Galaktosamin. Das bisher nicht mitgeteilte Vorkommen von Arabinose und Glucose in Seromucoid als auch die anderen durch Gaschromatographie bestimmten Kohlenhydrat-Komponenten wurden durch ihre Fragmentierungsmuster im Elektronenstoß-und chemischen lonisations-Massenspektrum bestätigt.

Exopolysaccharides produced by clinical strains belonging to the Burkholderia cepacia complex

“…Therefore, the high content of fucose and galactose could derive from the carbohydrate moiety of mucins. At the same time it was reported that the seromucoid fraction of human serum contained fucose, arabinose, mannose, glucose, galactose, glucosamine and galactosamine [36]. Rhamnose was not reported to be a component of the macromolecules mentioned above and therefore it is reasonable to correlate its presence with that of cepacian.…”

Evaluation of oxidative stress via total antioxidant status, sialic acid, malondialdehyde and RT‐PCR findings in sheep affected with bluetongue

“…Evaluation of the TSA concentration may be useful for diagnosing inflammatory diseases due to the increase in sialic acid during the inflammatory process, such as tissue degeneration and proliferation ( Singh and others 1980 , Haq and others 1993 , Citil and others 2004 ). Varma and others (1983) studied the protein-bound carbohydrates of seromucoid in normal human serum and reported that many acute phase proteins contain sialic acid. Due to acute phase proteins in the form of glycoprotein, an increase in these proteins in circulation affects the serum TSA level ( Taniuchi and others 1981 ).…”

Critical appraisal of the role of serum albumin in cardiovascular disease

“…This hypothesis is supported by the investigations on sialylated glycoproteins in serum. Some of the sialylated glycoproteins rapidly increase in response to the onset of inflammation and are regarded as an index of acute phase response [ 27 ].…”