The protective role of thymoquinone in the prevention of gentamicin ototoxicity

Sağıt, Mustafa; Korkmaz, Ferhat; Gürgen, Seren Gülşen; Kaya, Mesut; Akçadağ, Alper; Özcan, İbrahim

doi:10.1016/j.amjoto.2014.07.002

Cited by 14 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secondly, the antiinflammatory effect of TQ may have a neuroprotective effect, too. TQ exerts an anti-inflammatory effect through its inhibition of eicosanoid generation [17,41], and the use of TQ has been shown to have anti-inflammatory effects in several inflammatory diseases, including experimental allergic encephalomyelitis, colitis, arthritis, and bacterial prostatitis [12][13][14][15]17,[41][42][43][44]. As a result, we thought that marked neuroprotective effect with TQ in this study may be explained by anti-inflammatory effect of TQ in addition to its antioxidant effects.…”

Section: Discussionmentioning

confidence: 54%

The histopathological and electrophysiological effects of thymoquinone and methylprednisolone in a rabbit traumatic facial nerve paralysis model

Şereflican

Yurttaş

Özyalvaçlı

et al. 2016

American Journal of Otolaryngology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 54%

The histopathological and electrophysiological effects of thymoquinone and methylprednisolone in a rabbit traumatic facial nerve paralysis model

Şereflican

Yurttaş

Özyalvaçlı

et al. 2016

American Journal of Otolaryngology

View full text Add to dashboard Cite

“…Male LDL-R −/− mice (8-week-old) were randomly divided into the following three groups: the normal diet (ND, n = 8), high-fat diet (HFD, n = 8), and high-cholesterol diet+50 mg/kg/day of TQ (HFD+TQ, n = 8) groups [ 19 ]. Thymoquinone was dissolved in corn oil solution [ 20 ]. The HFD contained 1.2% cholesterol and 21% fat.…”

Section: Methodsmentioning

confidence: 99%

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects

Pei

Guo

Zhu

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Hyperlipidemia is a risk factor for cardiac damage and cardiovascular disease. Increasing evidence has shown that dyslipidemia-related cardiac damage is associated with lipid accumulation, oxidative stress, and inflammation. Thymoquinone (TQ) is the major constituent of Nigella sativa, commonly known as black seed or black cumin, and is globally used in folk (herbal) medicine for treating and preventing a number of diseases and conditions. Several studies have shown that TQ can protect against cardiac damage. This study is aimed at investigating the possible protective effects of TQ on hyperlipidemia-induced cardiac damage in low-density lipoprotein receptor-deficient (LDL-R-/-) mice. Eight-week-old male LDL-R-/- mice were randomly divided into normal diet (ND), high-fat diet (HFD), and HFD and TQ (HFD+TQ) groups and were fed the different diets for eight weeks. Blood samples were obtained from the inferior vena cava in serum tubes and stored at -80°C until use. Some cardiac tissues were fixed in 10% formalin and then embedded in paraffin for histological evaluation. The remainder of the cardiac tissues was snap-frozen in liquid nitrogen for mRNA preparation or immunoblotting. The levels of metabolism-related factors, such as total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-c), and high-sensitivity C-reactive protein (hs-CRP), were decreased in the HFD+TQ group compared with those in the HFD group. Periodic acid-Schiff staining demonstrated that lipid deposition was lower in the HFD+TQ group than in the HFD group. The expression of pyroptosis indicators (NOD-like receptor 3 (NLRP3), interleukin- (IL-) 1β, IL-18, and caspase-1), proinflammatory factors (IL-6 and tumor necrosis factor alpha (TNF-α)), and macrophage markers (cluster of differentiation (CD) 68) was significantly downregulated in the HFD+TQ group compared with that in the HFD group. Our results indicate that TQ may serve as a potential therapeutic agent for hyperlipidemia-induced cardiac damage.

show abstract

“…It is therefore possible that this represents a false positive. However, thymoqunone has previously been shown to protect HCs against damage due to gentamicin ( Sagit et al, 2014 ), cisplatin ( Sagit et al, 2013 ), and noise ( Aksoy et al, 2015 ) in vivo .…”

Section: Discussionmentioning

confidence: 99%

An Antioxidant Screen Identifies Candidates for Protection of Cochlear Hair Cells from Gentamicin Toxicity

Noack

Pak

Jalota

et al. 2017

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Reactive oxygen species are important elements in ototoxic damage to hair cells (HCs), appearing early in the damage process. Higher levels of natural antioxidants are positively correlated with resistance to ototoxins and many studies have shown that exogenous antioxidants can protect HCs from damage. While a very wide variety of antioxidants with different characteristics and intracellular targets exist, most ototoxicity studies have focused upon one or a few well-characterized compounds. Relatively little research has attempted to determine the comparative efficacy of large variety of different antioxidants. This has been in part due to the lack of translation between cell culture and in vivo measures of efficacy. To circumvent this limitation, we used an in vitro assay based on micro-explants from the basal and middle turns of the neonatal mouse organ of Corti to screen a commercial redox library of diverse antioxidant compounds for their ability to protect mammalian HCs from a high dose of the ototoxic antibiotic gentamicin. The library included several antioxidants that have previously been studied as potential treatments for HC damage, as well as many antioxidants that have never been applied to ototoxicity. The micro-explants were treated with 200 μM gentamicin alone, gentamicin plus one of three dosages of a redox compound, the highest dosage of compound alone, or were untreated. HC counts were determined before the gentamicin insult and at 1, 2, and 3 days afterward to evaluate the HC survival. From a total of 81 antioxidant compounds, 13 exhibited significant protection of HCs. These included members of a variety of antioxidant classes with several novel antioxidants, not previously tested on HCs, appearing to alleviate the damaging gentamicin effect. Some compounds previously shown to be protective of HCs were correspondingly protective in this in vitro screen, while others were not. Finally, one of the three pro-oxidant compounds included in the library as well as six antioxidants exhibited evidence of toxicity in the absence of gentamicin. The results demonstrate the wide variability in the ability of antioxidants to protect HCs from high-dose gentamicin damage, and identify promising candidate leads for further study as potential drug targets.Highlights• A medium-throughput assay based on micro-explants of the organ of Corti was developed to screen mammalian cochlear hair cells for protection from damage by ototoxins.• Eighty one antioxidants and 3 pro-oxidants were evaluated for hair cell protection from high-dose gentamicin.• Thirteen antioxidants were significantly protective, while 6 proved to be damaging.• The use of a common assay permitted an evaluation of the relative capacity of different antioxidants for the protection of hair cells.

show abstract

The protective role of thymoquinone in the prevention of gentamicin ototoxicity

Cited by 14 publications

References 25 publications

The histopathological and electrophysiological effects of thymoquinone and methylprednisolone in a rabbit traumatic facial nerve paralysis model

The histopathological and electrophysiological effects of thymoquinone and methylprednisolone in a rabbit traumatic facial nerve paralysis model

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects

An Antioxidant Screen Identifies Candidates for Protection of Cochlear Hair Cells from Gentamicin Toxicity

Contact Info

Product

Resources

About