The protective role of melatonin against the effects of different doses of caffeine on the fetus

Yılmaz, Seher; Göçmen, Ayşe Yeşim; Arikan, Evrim Suna; Akyüz, Enes; Tokpınar, Adem; Nisari, Mehtap; Unur, Erdoğan; Yay, Arzu; Yalçın, Betül; Yılmaz, Halil; Ertekın, Tolga; Güler, Hatice; Sabitaliyevich, Uteuliyev Yerzhan

doi:10.14715/cmb/2020.66.5.29

Cited by 6 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, Yılmaz et al clearly demonstrated powerful antioxidant and a protective role for MEL (10 mg/kg bw daily i.p.) against delayed ossi cation in Wistar Albino rat fetuses caused by the use of 60 mg/kg bw daily CAF during pregnancy [15]. More recently, Zhang et al also observed IUGR and maternal immune dysfunction in their study in which they administered CAF by gavage in a volume of 0 (control), 5, 20, and 80 mg/kg bw daily from the 6th day of pregnancy to lactation using CAF dissolved in water [16].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The protective effect of exogen melatonin upon fetal hippocampus damage caused by high-dose caffeine administration in pregnant rats

Köse

Şirin

Turgut

et al. 2023

Preprint

View full text Add to dashboard Cite

Objective: Caffeine (CAF), which is in the methylxanthines group (1,3,7-trimethylxanthine), is a neurologically active food component that is widely consumed and has a stimulating effect on the central nervous system. CAF taken in high doses during pregnancy rapidly crosses the placenta and causes many negative conditions such as low birth weight infants, premature births, spontaneous abortion, stillbirth, and principally fetal growth retardation. On the other hand, melatonin (MEL) is an endogenous hormone secreted from the pineal gland that plays a role in the regulation of many biological functions such as sleep, biological rhythm, reproduction, immunity and has neuroprotective effects. In this study, we aimed to investigate the possible effects of exogenous MEL on the fetal hippocampus damage caused by high-dose CAF administration in pregnant rats. Methods: In the study, 32 adult Wistar albino female rats were divided into four experimental groups after conception (n=8). No compound was administered to the control group. In the MEL group, intraperitoneal (i.p.) MEL was administered at a dose of 10 mg/kg/day at 9-20 days of pregnancy. In the CAF group, i.p. CAF was administered at a dose of 60 mg/kg/day at 9-20 days of pregnancy. In the CAF plus MEL group, i.p. CAF and MEL were administered at a dose of 60 mg/kg/day and 10 mg/kg/day, respectively at 9-20 days of pregnancy. Histological tissue processing procedures were performed after the extraction of the brains of the fetuses sacrificed on the 21st day of pregnancy. Hippocampal regions of fetal brains were analyzed by Hematoxylin and Eosin (H&E) and Cresyl Echt Violet (CEV) histochemical staining, anti-GFAP and anti-synaptophysin immunohistochemical staining methods. Results: While there was a decrease in fetal and brain weights in the CAF group, it was found that the CAF plus MEL group had a closer weight average to that of the control group. In histological examinations, while the MEL group showed similar characteristics with the control group, it was observed that the pyramidal cell layer consisted of 8-10 layers of cells due to the delay in migration in hippocampal neurons in the CAF group. It was found that these findings decreased in the CAF plus MEL group. Conclusion: Ultimately, it was determined that high-dose CAF administration caused delays and deterioration in neurogenesis in the fetal hippocampus region, and it was also shown that MEL administration was effective in reducing these findings.

show abstract

Section: Discussionmentioning

confidence: 99%

“…CAF can easily cross both the blood-brain barrier and the placental barrier [1,2,4,11,12]. Studies have reported that caffeine mixed with the drinking water of pregnant animals passes to the fetus [12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

The protective effect of exogen melatonin upon fetal hippocampus damage caused by high-dose caffeine administration in pregnant rats

Köse

Şirin

Turgut

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The images of the dyed fetuses placed on millimeter paper were recorded with a Nikon (5700) camera under a stereomicroscope. Finally, the images transferred to the computer were measured using the ImageJ program (https://imagej.nih.gov/ij/download.html) to measure the cartilaginous and ossification areas on the bones (Yılmaz et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Finally, the images transferred to the computer were measured using the ImageJ program (https://imagej.nih.gov/ij/download.html) to measure the cartilaginous and ossification areas on the bones (Yılmaz et al, 2020).…”

Section: Anesthesia and Tissue Samplingmentioning

confidence: 99%

See 1 more Smart Citation

The effect of ionizing radiation on the fetal bone development in pregnant rats: Role of melatonin

Tokpinar,

Nisari,

Yilmaz

et al. 2023

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

Radiation has been widely used in many business sectors over the last century. Our study investigated the possible teratogenic effects of radiation on the bones of rat fetuses and the protective effect of melatonin against these effects. In this study, 15 pregnant female Wistar albino rats were used. These rats were divided into four groups: the control group, melatonin group (10 mg/kg/day), radiation group (0.5 gray), radiation (0.5 gray) + melatonin group (10 mg/kg/day), and sham group (1 mm hanks/day). The skeletal system development of fetuses was examined with double skeletal and scanning electron microscope (SEM), histopathological methods. In our study, fetal weight, placental weight, and fetal morphometric values were found to be statistically significantly decreased in the radiation group compared to the control group (p < .05). In immünohistochemistry (IHC) analysis, alkaline phosphatase, and tartrate‐resistant acid phosphatase) concentrations were found to be significantly lower in the radiation group compared to the other groups. In the SEM analysis, it was observed that the amount of calcium and sodium decreased when the radiation group was compared with the other groups. As a result, when exposed to ionizing radiation during pregnancy, melatonin has a protective feature against the negative effects of radiation on the bone development of fetuses.Research Highlights In our study, fetuses obtained from pregnant rats exposed to ionizing radiation were examined. In this study, the effect of melatonin on bone development in fetuses exposed to gray ionizing radiation was investigated. There are few studies on our subject in the literature. We believe that our findings will contribute to other planned studies.

show abstract

Evaluation of the effect of tartrazine on the offspring rats in an in vivo experimental model

Öztürk,

Dikici,

Gür

et al. 2024

Food Science & Nutrition

View full text Add to dashboard Cite

Tartrazine, an azo dye prevalent in pharmaceuticals and food items, was investigated for its impact on fetal development, specifically examining visceral and skeletal abnormalities in rat offspring exposed to daily oral doses throughout pregnancy. Fourteen pregnant rats were randomly assigned to control and tartrazine groups (seven animals each), with tartrazine administered via oral gavage at 7.5 mg/kg throughout gestation. Offspring were categorized by gender for histopathological and genetic analysis of visceral structures. Bone quality and fracture resistance assessments involved micro‐CT, Raman spectroscopy, and biomechanical testing. Results highlighted distinct internal organ tissue differences in the tartrazine group, notably increased hemorrhagic and inflammatory cell infiltration, degeneration, and vacuolization compared to controls. Gender‐specific alterations in mRNA levels of IL‐6, IL‐1β, TNF‐α, and TRPM2 genes (p < .001) were also noted. Moreover, tartrazine‐exposed groups exhibited reduced trabecular thickness, bone volume, and significant alterations in bone matrix composition and quality alongside significant decreases in fracture resistance (p < 0.05). This study concludes that intrauterine exposure to tartrazine can result in adverse impacts on organ and bone development in rat offspring.

show abstract

The protective role of melatonin against the effects of different doses of caffeine on the fetus

Cited by 6 publications

References 44 publications

The protective effect of exogen melatonin upon fetal hippocampus damage caused by high-dose caffeine administration in pregnant rats

The protective effect of exogen melatonin upon fetal hippocampus damage caused by high-dose caffeine administration in pregnant rats

The effect of ionizing radiation on the fetal bone development in pregnant rats: Role of melatonin

Evaluation of the effect of tartrazine on the offspring rats in an in vivo experimental model

Contact Info

Product

Resources

About