The Protective Role of Feruloylserotonin in LPS-Induced HaCaT Cells

He, Yingkun; Kim, Byung-gook; Kim, Hye-Eun; Sun, Qiaochu; Shi, Shuhan; Ma, Guowu; Kim, Young; Kim, OkSu

doi:10.3390/molecules24173064

Cited by 12 publications

(17 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, inflammation of keratinocytes is associated with many skin problems. 2 Although inflammation is a prerequisite for healing, in the case of wounds and in general for skin restoration, uncontrolled inflammation can lead to tissue/skin damage and injuries. Consequently, alleviation of inflammatory injury is a pivotal step in the prevention of skin/tissue injury progression.…”

mentioning

confidence: 99%

Labdane-Type Diterpenes from the Aerial Parts of Rydingia persica: Their Absolute Configurations and Protective Effects on LPS-Induced Inflammation in Keratinocytes

et al. 2020

View full text Add to dashboard Cite

Phytochemical investigations of an extract of the aerial parts of Rydingia persica led to the isolation of 14 labdane-type diterpenoids, of which compounds 1 – 5 , 8 , and 12 – 14 turned out to be new natural products, while the remaining compounds were isolated for the first time from the genus Rydingia . Their structures were elucidated using 1D- and 2D-NMR and mass spectrometry, and their absolute configurations were determined by quantum chemical calculation methods. Furthermore, DP4+ NMR chemical shift probability calculations were performed for compounds 12 – 14 , in order to elucidate the orientation of the ambiguous chiral center at C-15, prior to absolute configuration determination. The methanol extract of the aerial parts of R. persica along with subfractions obtained and selected isolated compounds were evaluated for their effects on inflammation-related factors such as nitrotyrosine formation, IL-6 release, and TNF-α release, along with tight-junction proteins claudin-1 and occludin expression in LPS-stimulated HaCaT cells. Occludin and claudin-1 are tight-junction proteins, which play a pivotal role in wound repair mechanisms. Overall, the subfractions and compounds isolated showed moderate to high activity, indicating that labdane-type diterpenoids contribute to the anti-inflammatory and wound-healing activity of R. persica .

show abstract

mentioning

confidence: 99%

Labdane-Type Diterpenes from the Aerial Parts of Rydingia persica: Their Absolute Configurations and Protective Effects on LPS-Induced Inflammation in Keratinocytes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Apart from oxidative damage occurring with diabetic wounds, bacterial infection is another major cause that hinders the re-epithelization process (He et al 2019 ). Accordingly, it was decided to investigate whether CoPP pretreatment could enhance the motility of HaCaT while culturing the cells in HG media containing lipopolysaacharide (LPS).…”

Section: Resultsmentioning

confidence: 99%

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

Fang

Lai²,

Chen³

et al. 2022

Mol Med

View full text Add to dashboard Cite

Background Complete healing of diabetic wounds continues to be a clinically unmet need. Although robust therapies such as stem cell therapy and growth factor treatment are clinically applied, these treatments are costly for most diabetic wound patients. Therefore, a cheaper alternative is needed. Cobalt protoporphyrin (CoPP) has recently been demonstrated to promote tissue regeneration. In this study, the therapeutic benefits of CoPP in diabetic wound healing were examined. Methods An in vitro wound healing model that mimics re-epithelialization was established to examine the effect of CoPP on the migratory capability of human keratinocytes (HaCaT) in either normal glucose (NG) or high glucose (HG) media, as well as in the presence of either H2O2 or lipopolysaccharide (LPS). At the end of the migration assays, cells were collected and subjected to Western blotting analysis and immunostaining. Results HaCaT were found to migrate significantly more slowly in the HG media compared to the NG media. CoPP treatment was found to enhance cell migration in HG media, but was found to decrease cell migration and proliferation when HaCaT were cultured in NG media. CoPP treatment induced high levels of expression of Nrf-2/HO-1 and FoxO1 in HaCaT cultured in either glucose concentration, although the FoxO1 expression was found to be significantly higher in HaCaT that underwent the migration assay in NG media compared to those in HG media. The higher level of FoxO1 expression seen in CoPP-treated HaCaT cultured in NG media resulted in upregulation of CCL20 and downregulation of TGFβ1. In contrast, HaCaT migrated in HG media were found to have high levels of expression of TGFβ1, and low levels of expression of CCL20. Interestingly, in the presence of H2O2, CoPP-pretreated HaCaT cultured in either NG or HG media had similar expression level of Nrf-2/HO-1 and FoxO1 to each other. Moreover, the anti-apoptotic effect of CoPP pretreatment was noticed in HaCaT cultured in either glucose concentration. Additionally, CoPP pretreatment was shown to promote tight junction formation in HaCaT suffering from LPS-induced damage. Conclusions CoPP enhances cell migratory capacity under hyperglycemic conditions, and protects cells from oxidative and LPS-induced cellular damage in HG media containing either H2O2 or LPS.

show abstract

“…In LPS-stimulated cell lines, nuclear factor kappa B (NF-κB) is an important transcription factor in the expression of iNOS and COX-2 genes [ 33 , 34 ]. In LPS-induced human keratinocyte HaCaT cells, He et al [ 35 ] found that feruloylserotonin suppressed the toll-like receptor (TLR4)/NF-κB pathway and promoted the translocation of Nuclear factor-erythroid 2 related factor 2 (Nrf2). The chloroform fraction of Carpinus tschonoskii inhibited the protein and mRNA of chemokine in HaCaT cells by downregulating STAT1 in the IFN-γ signaling pathway [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Effect of Liverwort (Marchantia polymorpha L.) and Racomitrium Moss (Racomitrium canescens (Hedw.) Brid.) Growing in Korea

Kim

Hong

Kim

et al. 2021

Plants

View full text Add to dashboard Cite

Bryophytes contain a variety of bioactive metabolites, but studies about the anti-inflammatory effect of bryophytes are meager. Therefore, the present study aimed to compare the anti-inflammatory effect of methanol extract of Marchantia polymorpha L. (liverwort) and Racomitrium canescens (Racomitrium moss) in lipopolysaccharide (LPS)-induced HaCaT cells. To evaluate the anti-inflammatory effect of liverwort and Racomitrium moss, the levels of nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 and IL-1β in LPS-induced HaCaT cells were measured. The methanol extract of liverwort and Racomitrium moss significantly decreased LPS-induced NO production in HaCaT cells. When compared with Racomitrium moss extract, pre-treatment with methanol extract of liverwort markedly inhibited the expression of iNOS, COX-2, IL-6, and IL-1β at the concentration of 100 µg/mL with the exception of TNF-α. Further, liverwort extract markedly attenuated the production of TNF-α, IL-6, and IL-1β in the culture medium. In addition, ethyl acetate and butanol fractions obtained from the methanol extract of liverwort showed remarkable inhibitory activity against the production of NO in LPS-stimulated HaCaT cells. The LC-MS data revealed the presence of bisbibenzyl types of bioactive components in the methanol extract of liverwort. These data demonstrate that liverwort extract exhibits effective inhibitory activity against the production of inflammatory mediators in LPS-induced HaCaT cells and may be useful for the treatment of inflammation-mediated diseases.

show abstract

The Protective Role of Feruloylserotonin in LPS-Induced HaCaT Cells

Cited by 12 publications

References 23 publications

Labdane-Type Diterpenes from the Aerial Parts of Rydingia persica: Their Absolute Configurations and Protective Effects on LPS-Induced Inflammation in Keratinocytes

Labdane-Type Diterpenes from the Aerial Parts of Rydingia persica: Their Absolute Configurations and Protective Effects on LPS-Induced Inflammation in Keratinocytes

Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions

Anti-Inflammatory Effect of Liverwort (Marchantia polymorpha L.) and Racomitrium Moss (Racomitrium canescens (Hedw.) Brid.) Growing in Korea

Contact Info

Product

Resources

About