The Protective Effects of Intravenous Anesthetics and Verapamil in Gut Ischemia/Reperfusion-Induced Liver Injury

Kaplan, Necat; Yağmurdur, Hatice; Kılınç, Kamer; Baltaci, Bülent; Tezel, Savaş

doi:10.1213/01.ane.0000284696.99629.3a

Cited by 40 publications

(28 citation statements)

References 43 publications

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“…Although the precise mechanisms of intestinal I/R-induced liver injury have not been elucidated fully, previous research has shown that liver injury associated with intestinal I/R appear to be dependent on leukocyte adhesion and activation. Intestine-and/or liver-derived mediators, such as oxygen radical species, IL-6 and tumor necrosis factor-α, have been suggested as participants in the I/R-induced, leukocyte-mediated liver responses [5,7,21] . Recent studies have implied that the NF-κB pathway is involved in this process.…”

Section: Discussionmentioning

confidence: 99%

“…The liver is the first distant organ involved after intestinal I/R [4,5] . Although the detailed mechanism of liver injury induced by intestinal I/R remains to be elucidated, a variety of inflammatory mediators including intracellular adhesion molecule-1 (ICAM-1) and cytokines, as well as infiltration of neutrophils have been implicated in this process [6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

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Prophylaxis with carnosol attenuates liver injury induced by intestinal ischemia/reperfusion

Yao¹,

Zheng²,

Li³

et al. 2009

WJG

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AIM:To investigate the possible protective effects of carnosol on liver injury induced by intestinal ischemia reperfusion (I/R). METHODS:Rats were divided randomly into three experimental groups: sham, intestinal I/R and carnosol treatment (n = 18 each). The intestinal I/R model was established by clamping the superior mesenteric artery for 1 h. In the carnosol treatment group, surgery was performed as in the intestinal I/R group, with intraperitoneal administration of 3 mg/kg carnosol 1 h before the operation. At 2, 4 and 6 h after reperfusion, rats were killed and blood, intestine and liver tissue samples were obtained. Intestine and liver histology was investigated. Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and interleukin (IL)-6 were measured. Liver tissue superoxide dismutase (SOD) and myeloperoxidase (MPO) activity were assayed. The liver intercellular adhesion molecule-1 (ICAM-1) and nuclear factor κB (NF-κB) were determined by immunohistochemical analysis and western blot analysis. RESULTS:Intestinal I/R induced intestine and liver injury, characterized by histological changes, as well as a significant increase in serum AST and ALT levels. The activity of SOD in the liver tissue decreased after I/R, which was enhanced by carnosol pretreatment. In addition, compared with the control group, carnosol markedly reduced liver tissue MPO activity and serum IL-6 level, which was in parallel with the decreased level of liver ICAM-1 and NF-κB expression. CONCLUSION:Our results indicate that carnosol pretreatment attenuates liver injury induced by intestinal I/R, attributable to the antioxidant effect and inhibition of the NF-κB pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prophylaxis with carnosol attenuates liver injury induced by intestinal ischemia/reperfusion

Yao¹,

Zheng²,

Li³

et al. 2009

WJG

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“…7,8 Although gut IR is not characterised by early histological liver lesions, a subcellular approach might nevertheless throw light on a role of the liver in the deleterious effects induced by gut IR. 9 The study of liver mitochondria, in particular, might be useful as mitochondria are the main energy source in cells and convert nutrients into energy through cellular respiration. 10 Impaired mitochondrial function is strongly involved in IR physiopathology.…”

mentioning

confidence: 99%

Methylene Blue Protects Liver Oxidative Capacity after Gut Ischaemia–Reperfusion in the Rat

Collange

Charles

Bouitbir

et al. 2013

European Journal of Vascular and Endovascular Surgery

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“…Sections were cut at a thickness of 5 lm and stained with hematoxylin and eosin, and the stained sections were used to assess liver damage. Two independent blind observers graded the histological injury using a scoring system from 0 (none) to 4 (severe) previously described, 21,22 with modifications. Histopathological changes observed in representative hematoxylin and eosin stained liver sections were given histological scores based on the extent of hepatocellular injury as follows: 0: normal liver architecture; 1: minimal injury (swelling, congestion, single cell necrosis); 2: mild injury, with one or more minute foci of necrosis, the largest involving \ 1% of the examined sectional area of the lobule; 3: moderate injury, as in 2, but the necrotic foci occupying 1-5% of the lobule; and 4: severe injury, as above, but with necrotic foci covering [ 5% of the lobule.…”

Section: Histological Analysis Of Hepatocellular Damagementioning

confidence: 99%

L’hydroxyéthylamidon 130/0,4 atténue les dommages hépatiques précoces causés par les lésions d’ischémie/reperfusion

Catré

Viana

Cabrita

et al. 2010

Can J Anesth/J Can Anesth

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Purpose Ischemia/reperfusion injury (IRI) remains a clinical challenge. We tested the hypothesis that fluid therapy using hydroxyethyl starch (HES) 130/0.4 during the early phase of IRI in rat liver decreases markers of hepatic injury. Methods We induced liver IRI in three groups of rats anesthetized with ketamine and chlorpromazine by means of 60 min of segmental hepatic ischemia followed by 120 min of reperfusion. At the onset of reperfusion, Group 1 (IRI ? HES; n = 12) was given 13 mLÁkg -1 of HES; Group 2 (IRI ? HS; n = 12) received the same volume of 7.5% saline (HS), and Group 3 (IRI-only; n = 12) received no fluid. Three other groups of 12 animals each were shamoperated and received the same fluid as the test groups. We euthanized the animals after three hours, drew blood for alanine aminotransferase (ALT) quantification, and took ischemic liver samples for histomorphological study.Results Serum ALT activity was greater in all of the IRI groups than in the sham-operated animals. The ALT activity was 1,081 ± 575 IUÁL -1 in IRI ? HES Group 1; 2,363 ± 1,839 IUÁL -1 in IRI ? HS Group 2; and 2,866 ± 2,491 IUÁL -1 in IRI-only Group 3. There was a statistically significant difference between the IRI ? HES and the IRIonly groups (P = 0.001), but not between the IRI ? HS and the IRI-only groups (P [ 0.05). Likewise, histological scores were greater in all IRI groups compared with the sham-operated animals. Scores were higher in the IRI-only group (median 3.5) than in the groups receiving fluid (IRI ? HES median 2; IRI ? HS median 3). The difference between IRI ? HES and IRI-only was statistically significant (P = 0.008) but not so between IRI ? HS and IRI-only (P [ 0.05). Conclusions Giving HES 130/0.4 attenuates rat liver IRI compared with no fluid, while giving HS does not. This suggests a role for HES in hepatoprotection associated with liver IRI. RésuméObjectif La le´sion d'ische´mie/reperfusion (IR) reste un de´fi clinique. Nous avons e´mis l'hypothe`se qu'une the´rapie liquidienne a`base d'hydroxye´thylamidon (HES) 130/0,4 administre´e pendant la phase pre´coce d'IR dans un mode`le he´patique de rat, diminuerait les marqueurs de la le´sion he´patique. Méthode Nous avons provoque´une le´sion d'IR au niveau du foie dans trois groupes de rats anesthe´sie´s à la ke´tamine et a`la chlorpromazine en cre´ant une ische´mie he´patique segmentaire de 60 min suivie de 120 min de reperfusion. Au de´but de la reperfusion, le groupe 1 (IR ? HES; n = 12) a reçu 13 mLÁkg -1 de HES; le groupe 2 (IR ? HS; n = 12) a reçu le meˆme volume de se´rum physiologique 7,5 % (HS), et le groupe 3 (IR seule;This investigation was supported, in part, by research funds of the School of Medicine of the University of Coimbra (GAPI 08/08).

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The Protective Effects of Intravenous Anesthetics and Verapamil in Gut Ischemia/Reperfusion-Induced Liver Injury

Abstract: Propofol may offer advantages by inhibiting lipid peroxidation and inflammatory cytokine production in an animal model of gut ischemia/reperfusion-induced liver injury.

Cited by 40 publications

References 43 publications

Prophylaxis with carnosol attenuates liver injury induced by intestinal ischemia/reperfusion

Prophylaxis with carnosol attenuates liver injury induced by intestinal ischemia/reperfusion

Methylene Blue Protects Liver Oxidative Capacity after Gut Ischaemia–Reperfusion in the Rat

L’hydroxyéthylamidon 130/0,4 atténue les dommages hépatiques précoces causés par les lésions d’ischémie/reperfusion

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