The protective effects ofArctium lappaL. Extract on testicular injuries induced by ethanol in rats

Abstract

The aim of this study is to assess the antioxidant activity and also protective effects of Arctium lappa L. (AL) extract on ethanol-induced testicular injuries in rats. In addition, the total phenol and flavonoid contents of the methanolic extracts were analysed and the antioxidant activity of the extract was investigated. The studied extract possessed strong antioxidant activity (IC ; 0.113 ± 0.007 mg/ml) and exhibited more active than the ascorbic acid (IC ; 0.142 ± 0.002 mg/ml). The histological examinations revealed that the co-administration of ethanol and AL extract inhibits the testicular injuries, alterations in superoxide dismutase (SOD) activity, and H O and malondialdehyde (MDA) levels were observed in ethanol-treated rats in comparison to the control group. The obtained results demonstrated strong pharmaceutical activities of AL and its potential capacity to be developed as a new health-care supplement against ROS-related substances.

Effect of ethanol on the changes in testicular protein expression in adult male rats

“…Indeed, our result showed no difference in epididymal sperm concentration as compared between control and ethanol-treated animals for 14 consecutive days as shown in Table 1. In contrast, it was totally different from a previous study reported by Yari et al (2018) showing sig- to early affect spermatogenesis because some seminiferous epithelium obviously showed some histopathologies (Figure 4) with reduction of serum testosterone levels (Table 1). These results are also similar to those investigations previously observed by Yari et al (2018).…”

“…Indeed, it has been demonstrated that ethanol treatment in long period could decrease sperm amount in adult rats (Nishi et al, 2018;Yari et al, 2018).…”

“…In addition, ethanol has a detrimental effect on sexual behaviour and fertility (Ávila et al., 2016; Gude, 2012). Previous studies showed that ethanol consumption could affect male reproductive system in adult male rats (Nishi et al., 2018; Yari, Karamian, Asadbegy, Hoseini, & Moazzami Farida, 2018). Previous study showed damaging of seminiferous tubules and reduced testosterone levels in sexually mature rats treated with ethanol for 14 consecutive days (Yari et al., 2018).…”

Preventive effect of Stachys lavandulifolia against gentamicin-induced oxidative stress and nephrotoxicity in rats

“…The samples airdried and grounded were at room temperature without sunlight. The extract was prepared according to the method used by Yari et al (2018). The dried leaves (25 g) were extracted twice for 72 h in 1 L of ethanol 70% (v/v) using the Soxhlet apparatus.…”

Therapeutic Value of Arctium Lappa Linn. – A Review

“…AL L. possessed strong antioxidant activity (IC 50 ; 0.113±0.007 mg/ml) and exhibited more active than the ascorbic acid (IC 50 ; 0.142±0.002 mg/ml). The histological examinations revealed that the coadministration of ethanol and AL extract inhibits the testicular injuries, alterations in superoxide dismutase activity, and H 2 O 2 and malondialdehyde (MDA) levels were observed in ethanoltreated rats in comparison to the control group [50]. Treatment with the ethanol extract of AL L. roots improved working and reference memory in mice in the Y-maze and passive avoidance tests.…”