The protective effects of grape seed oil on induced osteoarthritis of the knee in male rat models

Abstract

Background Osteoarthritis (OA), though being treated via various methods and medicines, is still a major healthcare concern mostly due to the increase in diagnosis of these age-related diseases. The present study aimed at investigating the effects of oral and intra articular injection of grape seed oil on OA in male rat models. Methods and materials Seventy male rats were selected and their anterior cruciate ligament was cut to induce OA. They were divided into 7 groups (n = 10): C1, no treatment; C2, receiving 300 mg/day of Piascledine per os (PO); C3, 1 mg sodium hyaluronate intra-articularly in days 1, 7, 14; C4, 1 mg methyl-prednisolone acetate intra-articularly; E1, avocado and grape seed oil combination (2:1, 300 mg/day) PO; E2, 500 mg/day of grape seed oil PO; E3, 200 mg/day grape seed oil intra-articularly. After 10 weeks, the rats were anesthetized and evaluated radiologically and histopathologically. P value ≤ 0.05 was considered as statistically significant. Results All the groups made significant differences with C1 regarding all inspected radiological criteria (P ≤ 0.05). E1 and E3 showed significantly better effects on medial femoral condyle, medial tibial condyle, joint space width, total osteophyte, and OA scores (P ≤ 0.04). Joint surface, matrix, cell distribution, cell population viability, calcification, and subchondral bone in treatment groups had significantly better scores versus C1 (P ≤ 0.04). E1 and E3 had significantly superior results regarding joint surface, cell viability, and calcification (P ≤ 0.04). Conclusions Grape seed oil has protective effects, both in injectable form and PO in combination with avocado, on OA in rats. Further clinical trials are necessary.

Protective effect of ethanolic extract of Echinacea purpurea contained nanoparticles on meniscal/ligamentous injury induced osteoarthritis in obese male rats

“…Besides, anti-OA effects of Mollugo pentaphylla extract and Dipsacus asperoides extract were also reported 40,41 . The protective effect of grape seed oil on OA was also reported 42 . Mani et al ( 2016), used phyto-stabilized silver nanoparticles for arthritis treatment and Piper nigrum extract was used as a stabilizer 43 .…”

Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway

“…The OA disease is categorized via progression of articular cartilage loss and the growth of bone osteophytes at the joint borders 25 . The available treatments for OA disease are surgical procedure, medical and non-medical therapy, which are more focus on the symptom therapies such as reducing pain and enhancing the overall function 26 . However, due to limitations, the researcher focuses their research to scrutinize the more protective drug from the natural source.…”

The Wine Industry By-Products: Applications for Food Industry and Health Benefits

“…In vitro studies were also carried out with the unsaponifiable fraction of grape seed oil in LPS-treated human primary monocytes and murine macrophage cell line RAW 264.7i, and the antioxidant effects observed were attributed to the suppression of the intracellular production of ROS and nitrite levels as a consequence of reduced NO synthase 2 gene expression [ 127 ]. Due to its anti-inflammatory and antioxidant effects, recent in vivo studies show a promising role for grape seed oil in the prevention and/or treatment of cadmium-induced nephrotoxicity [ 128 ], osteoarthritis [ 129 ], and ulcerative colitis [ 130 ].…”