The protective effects of different compatibility proportions of the couplet medicines for Astragali Radix andAngelica sinensisRadix on myocardial infarction injury

Abstract

Context: Astragali Radix (AR) and Angelica sinensis Radix (ASR) combinations are used to treat cardiovascular disorders. Objectives: This study investigates the protective effects of different compatibility proportions of AR and ASR on cardiac dysfunction in a C57BL/6 mouse model of myocardial infarction (MI). Materials and methods: MI mice were induced by ligation of the left coronary artery and divided into six groups: sham, vehicle, 10 mg/kg/d simvastatin and combinations of AR and ASR at different ratios, including 1:1 (AR 2.5 g/kg þ ASR 2.5 g/kg), 3:1 (AR 3.75 g/kg þ ASR 1.25 g/kg) and 5:1 (AR 4.17 g/kg þ ASR 0.83 g/kg). Both AR-ASR combinations and simvastatin were dissolved in saline solution and given daily by gavage. The left ventricle function, infarct size, heart tissue injury, apoptosis of cardiomyocytes, leukocyte infiltrates, capillary density and expression of cleaved caspase-3, cleaved caspase-9, Bcl-2, Bax, Bad, IL-1b, IL-6, VEGF, p-Akt and p-eNOS were analysed. Results: Different combinations of AR and ASR improve cardiac function and reduce infarct size (61.15% vs. 39.3%, 42.65% and 45.5%) and tissue injury through different mechanisms. When AR was combined with ASR at ratio of 1:1, the inflammation and cardiomyocyte apoptosis were suppressed (p < 0.05, p < 0.01). The combination ratio of 3:1 exerted effect in promoting angiogensis (p < 0.05). In the combination of AR and ASR at 5:1 ratio, angiogenesis was significantly improved (p < 0.01) and the apoptosis was inhibited (p < 0.05). Conclusions: Our results reflect the regulation of multiple targets and links in herb pairs and provide an important basis for the use of AR and ASR combinations in the treatment of MI.

Ferulic Acid Protects Endothelial Cells from Hypoxia-Induced Injury by Regulating MicroRNA-92a

“…Ferulic acid (FA) (4-hydroxy-3-methoxy cinnamic acid) is the core active component in Angelica sinensis, which possesses three spiral structures, and is also largely found in several fruits and vegetables. In recent years, FA exhibits an extensive therapeutic impact, such as oxidation resistance, antiageing, anti-inflammation, insulin resistance, and neuroprotective [4][5][6][7].…”

Modified Taohong Siwu Decoction Improved Heart Function After Myocardial Infarction by Inhibiting Inflammatory Factor and Promoting Chemotactic and Pro-Angiogenic Factors

“…Radix Astragali has numerous cardioprotective effects, including reducing in ammatory factor injury and myocardial apoptosis, promoting cardiac microvessel formation and relieving excessive oxidative stress [15][16][17][18]. A recent study showed that the combination of Radix Astragali and Angelica Sinensis not only promoted angiogenesis but also suppressed in ammation and cardiomyocyte apoptosis in a mouse model of MI [19]. The traditional Chinese herb Epimedium has the properties of warming Yang and has been used for the remedy of cardiovascular disease as it has anti-heart failure effect.…”

Exploration of the Active Component and Mechanisms of Shengyu Decoction Against Myelosuppression Using Network Pharmacology and in vitro Experimental Validation

“…These extracts and compounds have a broad range of pharmacological activities, such as hemopoietic, immunoregulatory, anti-inflammatory, antibacterial, antiviral, antioxidant, and antitumor activities. [10][11][12][13] However, the primary effective substances of SYD against CIM and their regulatory mechanisms remain to be explored.…”