The protective effect of rutin against busulfan-induced testicular damage in adult rats

Mgbudom-Okah, Chidimma J; Onuah, Chigozie L

doi:10.1080/01480545.2020.1803905

Cited by 8 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The changes in organ and body weights observed in the busulfan‐treated rats may be due to the damaged tissue and a functional reduction caused by high production of ROS, as well as excessive break‐down of tissue proteins by hydroxyl ions (Mohammadghasemi et al., 2008). However, results obtained from this study showed a marked decrease in testicular and body weights decline in the busulfan‐treated rats, which are in accordance with the previous report (Abarikwu et al., 2020). Reversal treatment with extract of KV significantly rescued body and testicular weights.…”

Section: Discussionsupporting

confidence: 93%

“…Indeed, the brain and the testes are markedly defenseless and unprotected from oxidative stress probably because of reduced endogenous antioxidant defense systems (Ahles & Saykin, 2007; Aitken & Roman, 2008). From this study, busulfan decreased the activities of the testicular endogenous antioxidants (SOD, CAT, GSH, and GPx) with increased testicular and neuronal lipid peroxidation markers (MDA and 8OHdG), suggesting increased testicular oxidative damage (Abarikwu et al., 2020). However, we found significant elevation of testicular SOD, CAT, and GSH accompanied by decreased MDA concentrations in rats treated with KV extract.…”

Section: Discussionmentioning

confidence: 60%

“…Testicular oxidative stress due to chronic usage of cytotoxic drugs and environmental toxicants plays a negative role in several conditions related to fertility in males . Several studies on antineoplastic agents have Busulfan triggers oxidative stress, characterized by subtle and strong biological effects (Abarikwu et al, 2020). Oxidative damages have been involved and connected to many factors that exacerbate dysfunction in male reproductive organs (Abarikwu et al, 2020) and reports claim that there are strong correlations between increased oxidative stress and environmental toxicants or drug toxicity causing reproductive impairment (Abarikwu et al, 2019;Nasimi et al, 2018;Oyovwi, Ben-Azu, Tesi, et al, 2021;.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, Busulfan triggers oxidative stress, characterized by subtle and strong biological effects (Abarikwu et al., 2020). Oxidative damages have been involved and connected to many factors that exacerbate dysfunction in male reproductive organs (Abarikwu et al., 2020) and reports claim that there are strong correlations between increased oxidative stress and environmental toxicants or drug toxicity causing reproductive impairment (Abarikwu et al., 2019; Nasimi et al., 2018; Oyovwi, Ben‐Azu, Tesi, et al., 2021; Oyovwi, Nwangwa, et al., 2021). Thus, the deleterious adverse effects of busulfan in physiological systems are usually conveyed by ROS cellular production occurring from radiolysis of water and inhibition of thiol release (Probin et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies on antineoplastic agents have shown increased testicular oxidative stress activity resulting in disturbances of testicular functions (Abarikwu et al., 2019; Nasimi et al., 2018; Oyovwi, Ben‐Azu, Edesiri, et al., 2021). Notably, Busulfan triggers oxidative stress, characterized by subtle and strong biological effects (Abarikwu et al., 2020). Oxidative damages have been involved and connected to many factors that exacerbate dysfunction in male reproductive organs (Abarikwu et al., 2020) and reports claim that there are strong correlations between increased oxidative stress and environmental toxicants or drug toxicity causing reproductive impairment (Abarikwu et al., 2019; Nasimi et al., 2018; Oyovwi, Ben‐Azu, Tesi, et al., 2021; Oyovwi, Nwangwa, et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Kolaviron, a flavonoid‐rich extract ameliorates busulfan‐induced chemo‐brain and testicular damage in male rats through inhibition of oxidative stress, inflammatory, and apoptotic pathways

Tesi

Ben‐Azu

Mega

et al. 2022

Journal of Food Biochemistry

View full text Add to dashboard Cite

Previous reports revealed that increased oxidative stress with up-regulated inflammatory proteins and apoptotic factors have serious implications in busulfan-induced chemo-brain and testicular damages. Hence, we investigated the potential reversal effects of kolaviron (KV), a neuro-active extract rich in flavonoids with proven antioxido-inflammatory and anti-apoptotic properties, on busulfan-induced oxidative damage, inflammatory proteins, and apoptosis in the brains and testes of male rats. In the treatment-regimen, animals in groups 1 and 2 had saline (10 ml/kg/p.o./day) and dimethyl sulfoxide (DMSO; 10 ml/kg/p.o./day), group 3 received KV extract (200 mg/ kg/p.o./day), group 4 was given busulfan (50 mg/kg/p.o./day) and animals in group 5 were pretreated with busulfan (50 mg/kg/p.o./day) successively for 56 days in addition to KV extract (200 mg/kg/p.o./day) from days 29-56. Non-spatial memory function was valuated with a novel-object recognition memory test. Thereafter, testicular and brain oxidative/antioxidant status, proinflammatory-and apoptotic-related proteins, testicular enzymatic markers were evaluated respectively. Kolaviron extract improved cognitive function by increasing exploration of novel-object of busulfantreated rats. Kolaviron extract reversed busulfan-mediated increased malondialdehyde, 8-hydroxy-2′-deoxyguanosine, and decreased superoxide dismutase, catalase, glutathione, and glutathione-peroxidase in brains and testes as well as the testicular enzyme markers. Increased brain and testicular weights, and TNFα, IL-1β, and NFκβ productions due to busulfan administration were also reduced by the extract.The reduced testicular B-cell lymphoma-2, sperm mitochondrial cytochrome-C, and membrane potential, increased DNA fragmentation, caspases -3 and -9 levels were also profoundly reversed by KV. Additionally, KV extract ameliorated busulfaninduced testicular histopathological changes in rats. Conclusively, KV extract reverses busulfan-induced neuroendopathobiological derangements via oxidative stress inhibition, down-regulation of inflammatory and apoptotic mediators in rats' brains and testes.

show abstract

Section: Discussionsupporting

confidence: 93%