The protective effect of rutin against ischemia/reperfusion-associated hemodynamic alteration through antioxidant activity

Bhandary, Bidur; Piao, Cheng Shi; Kim, Do‐Sung; Lee, Geum-Hwa; Chae, Soo‐Wan; Kim, Hyung‐Ryong; Chae, Han-Jung

doi:10.1007/s12272-012-0617-6

Cited by 27 publications

(16 citation statements)

References 18 publications

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“…This indicates that influence of rutin on photochemical GFP behavior is mediated by intracellular biochemical pathways. Rutin and its analogs were previously described as efficient scavengers of radicals [9], [10]. In addition, it was recently demonstrated that rutin reacts with light-excited flavins [11].…”

Section: Resultsmentioning

confidence: 99%

Anti-Fading Media for Live Cell GFP Imaging

2012

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Photostability is one of the most important characteristic of a dye for fluorescence microscopy. Recently we demonstrated that vitamins present in imaging media dramatically accelerate photobleaching of Enhanced Green Fluorescent Protein (EGFP) and many other green fluorescent and photoactivatable proteins. Here we tested all vitamins of commonly used media (such as Dulbecco's Modified Eagle Medium, DMEM) one-by-one and found that only two vitamins, riboflavin and pyridoxal, decrease photostability of EGFP. Thus, DMEM without riboflavin and pyridoxal can be used as an imaging medium, which ensures high photostability of GFPs at the expense of minimal biochemical disturbance. Then, we tested some antioxidants and found that a plant flavonoid rutin greatly enhances photostability of EGFP during live cell microscopy. In complete DMEM, rutin increased EGFP photostability up to the level of vitamin-depleted DMEM. Moreover, being added to vitamin-depleted DMEM, rutin was able to further suppress EGFP photobleaching. Potentially, new medium formulations can be widely used for fluorescence microscopy of GFP-expressing cells and model multicellular organisms in a variety of imaging applications, where photostability represents a challenge.

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Section: Resultsmentioning

confidence: 99%

Anti-Fading Media for Live Cell GFP Imaging

2012

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“…SOD and CAT are major antioxidant enzymes, which are involved in protective effects in organ injury. In ischemia/reperfusionassociated hemodynamic alteration, rutin improves left ventricular end diastolic pressure by enhancing the antioxidant enzyme SOD activity (Bhandary et al, 2012). In addition, rutin has protective effects in amylin-induced neurocytotoxicity by increasing the activity of CAT, which suggests that rutin can be used as a feasible agent to protect the aging brain (Yu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Protective effects of rutin on lipopolysaccharide-induced heart injury in mice

Xianchu¹,

Zheng²,

Liu³

et al. 2018

J. Toxicol. Sci.

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Rutin has a wide range of beneficial health properties in the amelioration of multi-organ injury owing to its various biological effects. The aim of this study was to investigate the effects of rutin on lipopolysaccharide (LPS)-induced heart injury and clarify its potential cardioprotective mechanism. The mouse model of heart injury was intraperitoneal infection with LPS, and rutin was orally administered for 8 consecutive days. One day after LPS injection, heart histopathology, cardiac marker enzymes and cardiac fibrosis related genes were determined to evaluate the cardioprotective effects of rutin. In addition, oxidative parameters and inflammatory cytokines were tested to explore its possible underlying mechanism. The presented results showed that rutin significantly improved morphological changes of myocardium and relieved cardiac marker enzymes [creatine kinase (CK) and lactate dehydrogenase (LDH)] level to protect heart in LPS-induced sepsis. And more, rutin observably mitigated fibrosis related genes [matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9)] expression in the heart to prevent against LPS-induced cardiac fibrosis. In addition, rutin markedly increased antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)] activity, and improved oxidative production [malondialdehyde (MDA) and HO] level to balance the oxidation and anti-oxidation systems in the heart. Lastly, rutin dramatically ameliorated [tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6)] activity to restrain inflammatory responses in the heart. In conclusion, rutin possessed anti-oxidant and anti-inflammatory properties to improve LPS-induced heart injury, which suggested rutin could be used as a potential cardioprotective medicine in sepsis.

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“…In rats fed a high carbohydrate and fat diet, rutin attenuated metabolic changes and cardiac remodeling [ 56 ]. In control and diabetic rats, rutin had a cardioprotective role against ischaemia–reperfusion injury [ 16 , 57 ]. In a clinical setting, this flavonoid decreased systemic blood pressure in diabetic patients [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Rutin administration attenuates myocardial dysfunction in diabetic rats

Guimarães

Muzio

Rosa

et al. 2015

Cardiovasc Diabetol

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BackgroundOxidative stress plays a major role in diabetic cardiomyopathy pathogenesis. Anti-oxidant therapy has been investigated in preventing or treating several diabetic complications. However, anti-oxidant action on diabetic-induced cardiac remodeling is not completely clear. This study evaluated the effects of rutin, a flavonoid, on cardiac and myocardial function in diabetic rats.MethodsWistar rats were assigned into control (C, n = 14); control-rutin (C-R, n = 14); diabetes mellitus (DM, n = 16); and DM-rutin (DM-R, n = 16) groups. Seven days after inducing diabetes (streptozotocin, 60 mg/kg, i.p.), rutin was injected intraperitoneally once a week (50 mg/kg) for 7 weeks. Echocardiogram was performed and myocardial function assessed in left ventricular (LV) papillary muscles. Serum insulin concentration was measured by ELISA. Statistics: One-way ANOVA and Tukey’s post hoc test.ResultsGlycemia was higher in DM than DM-R and C and in DM-R than C-R. Insulin concentration was lower in diabetic groups than controls (C 2.45 ± 0.67; C-R 2.09 ± 0.52; DM 0.59 ± 0.18; DM-R 0.82 ± 0.21 ng/mL). Echocardiogram showed no differences between C-R and C. DM had increased LV systolic diameter compared to C, and increased left atrium diameter/body weight (BW) ratio and LV mass/BW ratio compared to C and DM-R. Septal wall thickness, LV diastolic diameter/BW ratio, and relative wall thickness were lower in DM-R than DM. Fractional shortening and posterior wall shortening velocity were lower in DM than C and DM-R. In papillary muscle preparation, DM and DM-R presented higher time to peak tension and time from peak tension to 50% relaxation than controls; time to peak tension was lower in DM-R than DM. Under 0.625 and 1.25 mM extracellular calcium concentrations, DM had higher developed tension than C.ConclusionRutin attenuates cardiac remodeling and left ventricular and myocardial dysfunction caused by streptozotocin-induced diabetes mellitus.

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The protective effect of rutin against ischemia/reperfusion-associated hemodynamic alteration through antioxidant activity

Cited by 27 publications

References 18 publications

Anti-Fading Media for Live Cell GFP Imaging

Anti-Fading Media for Live Cell GFP Imaging

Protective effects of rutin on lipopolysaccharide-induced heart injury in mice

Rutin administration attenuates myocardial dysfunction in diabetic rats

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