The Protective Effect of Recombinant Glycoprotein Vaccine Against HIRRV Infection.

Abstract

ABSTRACT--Hirame rhabdovirus (HIRRV) was first reported from flounder (Paralichthys olivaceus) in Japan, and has recently been isolated from an aquaculture farm in the Tongyoung area of Korea. Total RNA isolated from RTG-2 cells infected with HIRRV was used in RT-PCR to obtain a cDNA coding for the glycoprotein of HIRRV Korean strain, CA-9703. Around half sized G protein in the C-terminal region was expressed as glutathione-S-transferase fusion protein, and used as viral vaccine against HIRRV. Flounder fry were vaccinated with low and high doses of vaccine by immersing them for 7 min in vaccine solution.After 1 month, the fish were challenged with HIRRV either by immersion or intraperitoneal injection. Specific losses of fish treated with low and high vaccine doses by intraperitoneal injection challenge were both 6.7%, in contrast to 43.3% in non-vaccinated fish. Losses of vaccinated fish by immersion chal lenge were 1.7% and 0% for low and high vaccine doses, respectively, and 15% for the non-vacci nated control.

Induction of virus-specific cell-mediated cytotoxic responses of isogeneic ginbuna crucian carp, after oral immunization with inactivated virus

“…The viability of effector cells was always greater than 95%. 51 Cr-release assays were performed as previously described with slight modifications [17]. Briefly, target cells were plated at 10 4 collected with the Skatron supernatant harvesting system (Skatron Instruments Inc.) and radioactivities (counts per min) were measured in a COBRA II Auto-Gamma Counting System (Packard Instrument Co. Inc.).…”

Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

“…Sun et al has established a reverse transcription PCR based on the G gene, which could specifically detect the HIRRV from other viruses [9]. The G protein also contains antigenic determinants that can induce antibodies in fish [10]. The DNA vaccine containing G gene could induce protective immunity against HIRRV infection [11].…”

Development of a DNA vaccine against hirame rhabdovirus and analysis of the expression of immune-related genes after vaccination

“…Rainbow trout infected with an attenuated Aeromonas salmonicida strain expressing the glycoprotein epitope of infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) showed resistance when challenged with these viruses [6]. Further, the recombinant protein that was synthesized from a fragment of the HIRRV glycoprotein gene conferred protection to Japanese flounder against HIRRV infection [4]. However, the effect of recombinant protein as a vaccine is not yet well understood.…”

“…The pathogen is a negative stranded RNA virus and is classified into the genus Novirhabdovirus of the Rhabdoviridae family [3]. Eou et al [4] sequenced the whole genome of the HIRRV Korean strain, and it consists of 11 034 nucleotides. The virion of HIRRV consists of six viral proteins, namely, RNA polymerase, envelope glycoprotein, nucleocapsid protein, two matrix proteins and non-viral proteins [5].…”