The Protective Effect of Pomegranate Juice in Silver Nanoparticles Induced Hepatotoxicity in Mature Male Albino Mice

Abstract

Nano-silvers (AgNPs) are widely used in medical and consumer products thanks to their excellent antimicrobial and anti-carcinogenic effects. Sixty mature male albino mice were randomly distributed into six groups (ten per group). The first group was kept as negative control, the 2 nd one was injected with silver nanoparticles 78 mg/kg BW I/P for 14 days, group 3 was given pomegranate (20 ml/kg BW) orally for 28 days, in the fourth group pomegranate was administrated for 14 days, followed by AgNPs for another 14 days. The fifth one AgNPs were I/P injected for 14 days, followed by pomegranate for another 14 days. The last group received AgNPs and pomegranate on the same time with the same doses and route of previous groups. The results revealed that pomegranate has a protective impact on AgNPs toxicity in all groups. These results were clarified the findings of AST, ALT, SOD, CAT, MDA and GPX. The protective and treatment effects of pomegranate in hepatotoxicity were evidenced by regeneration of hepatocyte and kupffur cells.

An Overview of the Beneficial Role of Antioxidants in the Treatment of Nanoparticle‐Induced Toxicities

“…In another study, standardized G. biloba extract to 24% ginkgo flavonoids improved oxidative damage in the brain of rats treated with AgNPs, as well as significantly regulated proinflammatory cytokine gene expression in brain tissue [117]. Pomegranate [123] and beetroot [5] juices also provided significant hepatoprotective activities against AgNP-induced toxicity in animal experiments. Albrahim and Alonazi [5] showed that beetroot juice posttreatment has the potential to regulate apoptotic proteins p53 and Bcl-2 in liver tissue of rats treated with AgNPs.…”

In Vivo Study of Entero- and Hepatotoxicity of Silver Nanoparticles Stabilized with Benzyldimethyl-[3-myristoylamine)-propyl]ammonium Chloride (Miramistin) to CBF1 Mice upon Enteral Administration

“…Intraperitoneal administration of 33 nm AgNPs at a dose of 78 mg/kg to mice during the period of 14 days resulted in the pronounced increase in the ALT (+126.6%) and AST (+53.5%) levels, while the histological inspection of the liver revealed vascular hyperemia as well as parenchymatous and perivascular aggregations of mononuclear cells [ 33 ]. Another 30-day study where rats were injected intraperitoneally three times a week with 54.9 nm AgNPs revealed a severe hepatotoxic effect.…”