The Protective Effect of Matricaria chamomilla Extract on Histological Damage and Oxidative Stress Induced by Torsion/Detorsion in Adult Rat Ovary

Abstract

Introduction Ovarian torsion is one of the prevalent gynecologic emergencies, in which the adnexal components swing around their axis that leads to obstruction of arterial, venous, and/or lymphatic vessels (1). In this situation, gangrene and necrosis with the expansion of arterial insufficiency is followed by primary congestion and hemorrhagic infarction owing to venous and lymphatic obstruction. However, there is no data about the least number of turns to form the torsion and also there is no data for the duration of torsion that is necessary for the setting in of necrosis (2). Diagnosis of torsion is based on clinical indication and found by sonographic findings. Meanwhile a definite diagnosis is made only during surgery (1) Oophorectomy of the necrotic and/ or doubtful ovaries after torsion or incarceration is the preferred surgical treatment in contemporaneous practice. However, before maturity, the importance of losing the gonads is higher than its loss in later periods. Also, nonsynchronous contralateral torsion has an occurrence ranging between 2%-5%, therefore, is considered as a clinical catastrophe. Twenty-five percent of children with torsion have abnormal ovaries. Moreover, protection of the gonads is the main attention in children. Therefore, a more conservative approach has been recommended in the last research (2). In the past, remedial action to overcome ovarian torsion was surgical removal of the ovaries. Nowadays, due to the importance of the ovaries in fertility and secretion of sexual hormones, ovaries are retained (3). Ovarian torsion/detorsion or ischemia/reperfusion of ovary is a pathophysiologic event in which histological damage is associated with perfusion reduction followed by lack of oxygen (ischemia) in ovarian tissue. The inflammatory response induced by torsion/detorsion (reperfusion) can lead to vascular endothelial cells damage and microcirculation disorders that are largely responsible for the injury to the ovarian tissue. The damage caused by ischemia/reperfusion produces excessive reactive oxygen species (ROS) such as hydroxyl free radical species, hydrogen peroxide (H 2 O 2), and superoxide radical (4-6). Therefore, oxidation has wrecking effects on ovarian tissue, thus, antioxidant substances not only have great roles in the recovery process but also reverse the adverse effects. Natural products such as plant extracts are a good alternative for drugs used in oxidative damage (7).

Protective effects ofStevia rebaudianaaqueous extract on experimental unilateral testicular ischaemia/reperfusion injury in rats

“…These changes of MDA, GPX and SOD levels in T/D group were also observed in similar studies that treatment with some antioxidant substances like verapamil, onion juice, Matricaria chamomilla, thymoquinone and quercetin could improve this biochemical condition to approximately healthy condition (Aktoz, Kanter, & Aktas, 2010;Ameli et al, 2018;Ayan et al, 2016;Shokoohi, Madarek, et al, 2018;Soltani et al, 2018). There are two major limitations in this study.…”

“…(Chatsudthipong & Muanprasat, 2009;Jayaraman et al, 2008;Shivanna et al, 2013;Shukla et al, 2013Shukla et al, , 2009Tadhani et al, 2007;Tadhani & Subhash, 2006;Takahashi et al, 2001). (2018), Shokoohi, Madarek, et al (2018) and Soltani et al (2018) in separated studies indicated that I/R injury is accompanied by destruction in tissue morphology and reduction of MTBS, and this situation can be reversed by use of a material with antioxidant features (Ameli et al, 2018;Shokoohi, Madarek, et al, 2018;Soltani et al, 2018).…”

Protective effects of the hydroalcoholic extract of Fumaria parviflora on testicular injury induced by torsion/detorsion in adult rats

“…Based on our previous studies (Moghimian, Soltani, Abtahi, & Shokoohi, ; Soltani, Moghimian, Abtahi, & Shokoohi, ), the serum level of malondialdehyde (MDA) was measured by placing 0.20 cm³ of serum into a microtube containing 3.0 cm³ of glacial acetic acid, to which 3.0 cm³ of 1% TBA in 2% NaOH was added. The microtube containing the mixture was placed in boiling water for 15 min.…”

The effect of clomiphene citrate and human chorionic gonadotropin on the expression of CatSper1, CatSper2, LHCGR, and SF1 genes, as well as the structural changes in testicular tissue of adult rats

“…Such sensitivity influences the process of spermatogenesis, leading to the impairment in fertility. The evaluation of the properties of seminiferous tubules, that is, morphological and morphometric parameters is a simple method for the assessment of the changes in the spermatogenesis process (Altoé et al, ; Soltani et al, ). The spermatogenic process could be decreased by detrimental alterations in the function of stem cells, resulting in degeneration of spermatogonial cells and phagocytosis by Sertoli cells.…”