The protective effect of esculetin against aluminium chloride‐induced reproductive toxicity in rats

Türk, Erdinç; Tekeli, İbrahim Ozan; Özkan, Hüseyin; Uyar, Ahmet; Cellat, Mustafa; Kuzu, Müslüm; Yavaş, İlker; Yegani, Arash Alizadeh; Yaman, Turan; Güvenç, Mehmet

doi:10.1111/and.13930

Cited by 18 publications

(10 citation statements)

References 64 publications

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“…[55,56] Previous research has found esculetin to have anti-inflammatory properties. [37,57] These anti-inflammatory properties of esculetin are thought to be due to its antioxidant properties, which suppress inflammatory cell activation and migration by increasing defense against lipid peroxidation.…”

Section: Histopathological Resultsmentioning

confidence: 99%

Protective effects of esculetin against ovary ischemia–reperfusion injury model in rats

Güvenç,

Yüksel,

Kutlu

et al. 2023

J Biochem & Molecular Tox

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AimsOvarian ischemia–reperfusion (I/R) injury is a phenomenon that necessitates urgent intervention, which occurs as a result of ovarian torsion, and it is frequently seen in young women. A large amount of free radical and oxidative damage as a result of I/R plays a role in the cause of the incident. Antioxidant agents are thought to be beneficial in preventing this damage, and the potential protective effects of esculetin, which had not been tested previously, were investigated in this study.Study DesignThe rats in the study were divided into five groups at random: control, sham, esculetin, I/R, and treatment. Oxidative stress parameters, proinflammatory cytokines, nuclear factor erythroid 2‐related factor 2 (Nrf‐2)/nuclear factor‐kβ (NF‐κβ) pathway, and histopathological analyses were evaluated at the end of the study.Key FindingsAfter I/R, malondialdehyde levels, proinflammatory cytokines, tumor necrosis factor‐α and interleukin‐1β levels and NF‐κβ expressions were increased, Nrf‐2 expression and glutathione level decreased and the histopathologic picture deteriorated. However, as a result of the esculetin treatment, ameliorative effects in the aforementioned parameters were determined, and it was ensured that they returned to normal levels.ConclusionAccording to these findings, esculetin has protective effects on I/R damage by lowering lipid peroxidation and having antioxidant and anti‐inflammatory properties.SignificanceOur results proved the protective effect of esculetin against ovarian IR injury in rats and this may be attributed to Nrf‐2/NF‐κβ axis which showed antioxidant and anti‐inflammatory effects. Therefore, esculetin can be used in the future for preventive effects to ovarian IR injury.

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Section: Histopathological Resultsmentioning

confidence: 99%

Protective effects of esculetin against ovary ischemia–reperfusion injury model in rats

Güvenç,

Yüksel,

Kutlu

et al. 2023

J Biochem & Molecular Tox

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“…Consumption of water with a high aluminum content caused a decrease in cognitive and blood functions but had no effect on the kidneys of Bangka Island residents, in Indonesia (Irfannuddin et al, 2019). Al is also found in dust particles, food additives, antacids, buffered analgesics, antiperspirants, cooking vessels, tinned boxes, infant formulas, cosmetics, mining, drinking water, and several herbal preparations (Angelova et al, 2019; Türk et al, 2021), manufacturing, food, composed of kitchen utensils, medicines, cosmetics, food additives (Kumar et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Histological changes in liver and kidney of male mice by age after exposure to aluminum chloride

Irnidayanti,

Fatona,

Rizkawati

2023

Toxicol Ind Health

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The informal aluminum industry is increasingly widespread in low- and middle-income countries, especially in Indonesia. Aluminum exposure is a serious public health problem, especially among workers in the informal aluminum foundry sector. Research on aluminum (Al) is important to advance our understanding of its impact on physiological systems. Here, we investigated the effect of exposure to aluminum longitudinal histological changes on the liver and kidneys of male mice. Mice were separated into six groups (4/group): group 1, group 2, group 3 received vehicles, and group 4, group 5, group 6 were administered a single dose of Al at 200 mg/kg b.w. by intraperitoneally every 3 days for 4 weeks. Post-sacrifice, kidneys and liver were isolated for examination. While Al did not impact the body weight gain of male mice across all groups, it caused liver damage including sinusoidal dilatation, enlarged central veins, vacuolar degeneration, and pyknotic nuclei in one-month-old mice. Furthermore, atrophied glomeruli, blood-filled spaces, and disintegration of renal tubular epithelium are evident at one-month-age. By contrast, sinusoidal dilatation and enlarged central veins were found in mice two- and three-months-old, including hemorrhage in mice (two-month-old) and atrophy of glomeruli. Lastly, the kidneys of three-month-old mice displayed interstitial fibrosis and increasing mesenchyme in the glomeruli. In summary, we demonstrated that Al provoked histological changes in the liver and kidneys with Al-treated 1-month mice being the most susceptible.

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“…Metals are ubiquitous and have multiple industrial uses [1]. They persist in nature for long, making it difficult for humans to avoid metal exposure [2].…”

Section: Introductionmentioning

confidence: 99%

“…They persist in nature for long, making it difficult for humans to avoid metal exposure [2]. Aluminum is found in airborne dust particles, food additives, phosphate binders, antacids, buffered analgesics, antiperspirants, cooking vessels, tinned boxes, infant formulas, cosmetics, mining, drinking water, and several herbal preparations [1,3]. For example, aluminum chloride (AlCl 3 ) is present in many manufactured consumables and is considered a toxic element [4].…”

Section: Introductionmentioning

confidence: 99%

“…One of the most conspicuous health effects of aluminum is a decrease in male fertility rates [1]. For example, Berihu [14] and Pandey and Jain [15] reported that aluminum accumulation causes male reproductive toxicity through various processes, including inducing oxidative stress, interfering with spermatogenesis and steroidogenesis, altering cell communication, disrupting the blood-testis barrier, and influencing the endocrine system.…”

Section: Introductionmentioning

confidence: 99%

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Aqueous extract of bay leaf (Laurus nobilis) ameliorates testicular toxicity induced by aluminum chloride in rats

et al. 2022

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Background and Aim: Human exposure to aluminum is inevitable, and one of the most adverse health effects of aluminum is a decrease in male fertility rates. Therefore, this study investigated the ameliorative effects of an aqueous extract from Laurus nobilis-bay leaf (BL) on aluminum chloride (AlCl3)-induced testicular toxicity in rats. Materials and Methods: Twenty-four Wistar rats were divided into four groups (n = 6, each group): The control (group 1) received normal saline; Group 2 animals were intraperitoneally administered with 30 mg/kg body weight (BW) AlCl3; and Groups 3 and 4 were co-administered AlCl3 with 125 or 250 mg/kg BW of BL extract, respectively, for 21 days. Testes, epididymis, and blood samples were collected. Testicular plasma enzyme activity was measured using a spectrophotometric assay, while concentrations of inflammatory biomarkers were determined using enzyme-linked immunosorbent assay kits. Results: There was a significant increase (p < 0.05) in testicular enzyme activity in the group treated with AlCl3. However, there was no significant (p > 0.05) difference in testicular enzyme activity in groups co-administered AlCl3 and BL extract as compared with that in control. There was a significant (p < 0.05) increase in testicular nitrite concentration in the AlCl3-treated group, whereas the administration of BL extract significantly (p < 0.05) decreased nitrite concentration in Groups 3 and 4. Furthermore, the administration of BL extracts increased sperm count and improved the morphology of the testes in AlCl3-treated rats. Flavonoids, phenolic compounds, alkaloids, tannin, glycosides, saponin, anthraquinones, and steroids were identified in BL extract, with alkaloids and glycosides being the most abundant. Conclusion: Aqueous extract from BL ameliorated the toxic effect of AlCl3 and exhibited anti-inflammatory properties by inhibiting nitrite production while improving sperm count and morphology in AlCl3-treated rats. The bioactivity of the extract may be attributed to the presence of a wide range of phytochemicals. Therefore, BL aqueous extract could be a promising source of novel compounds with male fertility-promoting and anti-inflammatory properties.

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The protective effect of esculetin against aluminium chloride‐induced reproductive toxicity in rats

Cited by 18 publications

References 64 publications

Protective effects of esculetin against ovary ischemia–reperfusion injury model in rats

Protective effects of esculetin against ovary ischemia–reperfusion injury model in rats

Histological changes in liver and kidney of male mice by age after exposure to aluminum chloride

Aqueous extract of bay leaf (Laurus nobilis) ameliorates testicular toxicity induced by aluminum chloride in rats

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