The Protective Effect of CD8+CD28− T Suppressor Cells On The Acute Rejection Responses in Rat Liver Transplantation

Liu, Y.; Chen, N.; Chen, G.; Peng, You

doi:10.1016/j.transproceed.2007.06.089

Cited by 16 publications

(10 citation statements)

References 32 publications

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“…CD4 + CD25 + Tregs have been established as key regulatory cells in the control of autoimmunity, allergy and organ transplantation tolerance [5][6][7]9,10]. Not only CD4 + CD25 + Tregs, but also CD8 + Tregs have been reported to prevent the liver graft rejection [5,6,11,12]. For clinical liver transplantation, the identification of the precise mechanisms underlying liver allograft tolerance is essential to minimize or avoid the need of immunosuppressive treatment in patients undergoing liver transplantation.…”

Section: Discussionmentioning

confidence: 99%

CD8+CD103+ regulatory T cells in spontaneous tolerance of liver allografts

Lü

et al. 2009

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

CD8+CD103+ regulatory T cells in spontaneous tolerance of liver allografts

Lü

et al. 2009

International Immunopharmacology

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“…Conversely, T suppressor (Ts) cells are identified by the phenotype CD8 + CD28 − , and exert their regulatory effect mainly through the induction of tolerogenic dendritic cells (25). These cells share some markers with nTreg cells, mainly the expression of Foxp3 (26,27), and seem to play an important role in oral and transplant tolerance as well as in the regulation of gut inflammation (28).…”

Section: Introductionmentioning

confidence: 99%

Regulatory T Cells in children with intestinal parasite infection

García-Hernández

Alvarado‐Sánchez

Calvo-Turrubiartes

et al. 2009

Parasite Immunology

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Chronic intestinal parasite infection can induce both persistent immune activation and defective responsiveness of T cells. This study aimed to assess the number and function of T regulatory (Treg) cells in children with intestinal parasite infection. We have studied the peripheral blood from 93 children, 53 of them parasitized with protozoa, helminths, or both; the remainder were non parasitized, healthy controls. The number and function of CD4(+) CD25(high) and CD4(+) Foxp3(+) cells were similar in parasitized and control children. In contrast, there was a significant increase in the levels of CD3(+) CD69(+), CD4(+) CTLA-4(+), and CD8(+) CD28(-) T cells in helminth infected children. Moreover, some of these patients showed a diminished response to CD3/CD28 stimulation in comparison with the control children. Our data strongly suggest that whilst Treg cells are not affected by intestinal parasite infection, CD3(+) CD69(+), CD4(+) CTLA-4(+) and CD8(+) CD28(-) lymphocytes may play an important, but as yet undetermined role in the diminished immune competence observed in parasitized children.

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“…A, Glycemia was monitored over time in the recipients of rat islets alone (n = 5 mice), rat islets with DC (n = 8 mice), or rats islets with IL-10 DC (n = 8 mice). B, Xenograft survival curves were created from raw data by the Kaplan-Meier method, and statistical comparison between groups was performed using the Log-rank (Mantel-Cox) method which contribute to stable graft function in human and rodent liver transplantation, 54,55 and inhibit T-cell proliferation in xenotransplantation. 56,57 Injection of autologous tolerogenic DC induced skin allograft tolerance by cross-presentation of donor antigens and induction of CD8 + regulatory T cells.…”

Section: Discussionmentioning

confidence: 99%

Prolongation of rat‐to‐mouse islets xenograft survival by co‐transplantation of autologous IL‐10 differentiated murine tolerogenic dendritic cells

Madelon¹,

Montanari

Gruaz³

et al. 2020

Xenotransplantation

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Background Tolerogenic dendritic cells (DCs) represent a promising approach to promote transplantation tolerance. In this study, the potential of autologous bone marrow (BM)–derived murine DC to protect rat‐to‐mouse islets xenografts was analyzed. Methods Tolerogenic DCs were generated by differentiating BM cells in the presence of granulocyte‐macrophage colony‐stimulating factor and interleukin 10 (IL‐10, IL‐10 DC). The phenotype of IL‐10 DC was characterized in vitro by expression of costimulatory/inhibitory molecules (flow cytometry) and cytokines (Luminex and ELISA), their function by phagocytosis and T‐cell stimulation assays. To study transplant tolerance in vivo, rat islets were transplanted alone or in combination with autologous murine IL‐10 DC under the kidney capsule of streptozotocin‐induced diabetic C57BL/6 mice. Xenograft survival was evaluated by monitoring glycemia, cellular infiltration of xenografts by microscopy and flow cytometry 10 days post‐transplantation. Results Compared with control DC, IL‐10 DC exhibited lower levels of major histocompatibility complex class II, costimulatory molecules (CD40, CD86, CD205), lower production of pro‐inflammatory cytokines (IL‐12p70, TNF, IL‐6), and higher production of IL‐10. Phagocytosis of xenogeneic rat splenocytes was not impaired in IL‐10 DC, whereas stimulation of T‐cell proliferation was reduced in the presence of IL‐10 DC. Xenograft survival of rat islets in diabetic mice co‐transplanted with autologous murine IL‐10 DC was significantly prolonged from 12 to 21 days, without additional immunosuppressive treatment. Overall, infiltration of xenografts by T cells and myeloid cells was not different in IL‐10 DC recipient mice, but enriched for CD8+ T cells and myeloid cells with suppressor‐associated phenotype. Conclusions Autologous IL‐10‐differentiated DC with tolerogenic properties prolong rat‐to‐mouse islets xenograft survival, potentially by locally inducing immune regulatory cells, indicating their potential for regulatory immune cell therapy in xenotransplantation.

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The Protective Effect of CD8+CD28− T Suppressor Cells On The Acute Rejection Responses in Rat Liver Transplantation

Cited by 16 publications

References 32 publications

CD8+CD103+ regulatory T cells in spontaneous tolerance of liver allografts

CD8+CD103+ regulatory T cells in spontaneous tolerance of liver allografts

Regulatory T Cells in children with intestinal parasite infection

Prolongation of rat‐to‐mouse islets xenograft survival by co‐transplantation of autologous IL‐10 differentiated murine tolerogenic dendritic cells

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