The protective effect of Buffalo’s milk against toluene induced-nephrotoxicity in rats

Abstract

Background: Toluene is widely used in different activities of industrial, commercial and household applications. It can cause damage to the human body. Buffalos’ milk has a good nutritive value. Objectives: The aim of this study is to examine the negative effects of toluene on kidney tissues and to investigate the protective effects of buffalo’s milk against toluene-induced nephrotoxicity in rats. Materials and Methods: Forty adult male Wistar rats (180-220 g weight) were randomly assigned to eight groups (n = 5). Animals in groups I to IV received oral gavage 1 mL distilled water (DH2O) and groups V to VIII received oral gavage 1 mL buffalo’s milk. Ten minutes later, animals were received toluene (i.p) at doses of 300 mg/kg (groups I and V), 600 mg/kg (groups of II and VI), and 900 mg/kg (groups of III and VII), respectively. The animals in groups IV (control) and VIII were injected vehicle (corn oil) only. The experiment repeated for seven consecutive days. Twenty-four hours after the last administration, animals were killed with overdose of sodium pentobarbital. Blood samples were analyzed for blood urea nitrogen (BUN) and creatinine (Cr). One part of the kidney tissues were excised for measuring the activities of superoxide dismutase (SOD), glutathione reductase (GR) and catalase (CAT) and the level of malondialdehyde (MDA). Another parts were excised for histopatholgical examination. Results: Administration of toluene to male rats produced dose-dependent damage in the kidney. This was noted by elevation of BUN, Cr and MDA levels. In contrast, diminished the CAT, GR and SOD enzyme activities in rats treated with toluene when compared to those in control animals. Histopathological manifestations were also observed in dose related manner in toluene-treated rats. Buffalo’s milk had no effect on the biochemical parameters and kidney morphology when compared to those in control. However, it was able to prevent rat kidney against toluene toxicity. Conclusions: The results of this study demonstrated that toluene damages kidney tissue and is a nephrotoxic substance. Buffalo’s milk was able to prevent the renal damage as an antioxidant and a nephroprotective agent.

Impact of buffalo’s milk on rat kidney exposed to noise

“…He suggested that the protecting impact of camel's milk could be related to its antioxidant capacity (32). The antioxidant property and protective effect of buffalo's milk on toluene induced nephrotoxicity were also reported (33). Our data showed that pretreatment of rats with buffalo's milk before to noise exposure diminished noise induced renal injury.…”

Protective Role of Aqueous Extract of Atriplex Halimus L. Against Benzene-Induced Damage on Renal Function and Glomerular Cells in Rats

“…The association between food and health is well established. Recent overwhelming attention to plant products and traditional medicine has focused on natural antioxidant for preventing the tissues from various xenobiotics induced toxicities [13,14]. Atriplex halimus L. (Ah) is a famous halophytic shrub for the nutritive and energetic value, not only for livestock but also as food for nomads and the local steppe population.…”

Effect of Buffalo Milk on Rat’s Lung Exposed to Noise

“…Water buffalo milk is consumed in many countries. It contains high levels of vitamin A, iron, copper, phospho-rous, magnesium and other trace elements with antioxidant activity, as well (9)(10)(11)(12).…”

“…The animals of the third group were exposed to 100 dB noise for four hours daily within two consecutive weeks and the rats of the forth group were treated with 1 mL/d BM daily for two consecutive weeks (without noise exposure). The methods and amount of milk intake and also the selected sound intensity were based on the previous studies (12)(13)(14)(15)(16).…”