The protective effect of albumin on bevacizumab activity and stability in PLGA nanoparticles intended for retinal and choroidal neovascularization treatments

Polymeric micro/nanoparticles: Particle design and potential vaccine delivery applications

“…The major loading patterns are adsorption and encapsulation, which influence the bioactivity and release rate of antigen in a complex manner. On one hand, when antigens are microencapsulated with particle, inactivation of antigen may occur during this process and compromise the corresponding immunogenicity and even cause the adverse effect [54,55]. In contrast, antigens that formulated with particles by gentle adsorption process are expected to get a better chance to maintain their activities (the activity loss of adsorbed antigen on PLA NP is below 5%).…”

Recent Advances in Protein and Peptide Drug Delivery: A Special Emphasis on Polymeric Nanoparticles

“…Addition of stabilizing excipients has been widely employed to stabilize protein at aqueous-organic interface. Excipients such as bovine serum albumin (BSA), sugars and polyethylene glycol (PEG) have demonstrated protein stabilization effect during NPs formulation [27-29]. As an alternative to w/o/w method, solid-in oil-in water (s/o/w) method is also used in order to avoid water-organic solvent interface and related protein denaturation.…”

Recent advances in topical delivery of proteins and peptides mediated by soft matter nanocarriers

“…Among others, proteinic excipients such as albumin exhibit protective effects in different steps of protein encapsulation. Hence, Varshochian et al tested different stabilizers for the fabrication of PLGA nanoparticles loaded with bevacizumab using the water/oil emulsion method which can lead to aggregation and inactivation of the biomacromolecule triggered by interfacial adsorption (Varshochian et al, 2013). Albumin efficiently protected bevacizumab from entrapment stress and resulted in significantly enhanced stability due to its surface active properties.…”