The protective and healing effects of Si Shen Wan in trinitrobenzene sulphonic acid-induced colitis

Liu, Duan-Yong; Guan, Yongmei; Zhao, Hai-Mei; Yan, Dongmei; Tong, Wen-ting; Wan, Panting; Zhu, Weifeng; Liu, Hongning; Liang, Xin-Li

doi:10.1016/j.jep.2012.05.060

Cited by 25 publications

(26 citation statements)

References 29 publications

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“…The evidences of SSW therapeutic effects were reported in our previous study including decreased colon wet weight, colon organ coefficient, and colonic damage score; shorted colon length; improved pathological injury [20]. In the present study, TNBS administration successfully induced colonic mucosal injuries in mice, as evidenced by microscopic manifestations.…”

Section: Discussionsupporting

confidence: 75%

“…The water extract was filtered and evaporated by rotary evaporation below 60°C under reduced pressure. And then the residue was freeze-dried; the powder was taken (25.2% w/w of the crude drugs), stored at 4°C, and dissolved at the desired concentration with distilled water before use [20]. The quality control of SSW extract was reported in our previous article [20].…”

Section: Methodsmentioning

confidence: 99%

“…And then the residue was freeze-dried; the powder was taken (25.2% w/w of the crude drugs), stored at 4°C, and dissolved at the desired concentration with distilled water before use [20]. The quality control of SSW extract was reported in our previous article [20]. And SSW powder in the present study was the same batch as in our previous study.…”

Section: Methodsmentioning

confidence: 99%

“…The other specimens were processed for paraffin sectioning and hematoxylin-eosin (HE) staining ( n = 8). Histological injuries were evaluated according to the previously described scales [20, 21], taking into consideration both inflammatory cell infiltration and tissue damage. Scores for infiltration: 0, no infiltration; 1, increased number of inflammatory cells in the lamina propria; 2, inflammatory cells extending into the submucosa; 3, transmural inflammatory infiltrates; and for tissue damage: 0, no mucosal damage; 1, discrete epithelial lesions; 2, erosions or focal ulcerations; 3, severe mucosal damage with extensive ulceration extending into the bowel wall.…”

Section: Methodsmentioning

confidence: 99%

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Si Shen Wan Inhibits mRNA Expression of Apoptosis-Related Molecules in p38 MAPK Signal Pathway in Mice with Colitis

Zhao

Huang

Zhou

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Si Shen Wan (SSW) is used to effectively treat ulcerative colitis (UC) as a formula of traditional Chinese medicine. To explore the mechanism of SSW-inhibited apoptosis of colonic epithelial cell, the study observed mRNA expression of apoptosis-related molecules in p38 MAPK signal pathway in colonic mucosa in colitis mice treated with SSW. Experimental colitis was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in mice; meanwhile, the mice were administrated daily either SSW (5 g/kg) or p38 MAPK inhibitor (2 mg/kg) or vehicle (physiological saline) for 10 days. While microscopical evaluation was observed, apoptosis rate of colonic epithelial cell and mRNA expression of apoptosis-related molecules were tested. Compared with colitis mice without treatment, SSW alleviated colonic mucosal injuries and decreased apoptosis rate of colonic epithelial cell, while the mRNA expressions of p38 MAPK, p53, caspase-3, c-jun, c-fos, Bax, and TNF-α were decreased in the colonic mucosa in colitis mice treated with SSW, and Bcl-2 mRNA and the ratio of Bcl-2/Bax were increased. The present study demonstrated that SSW inhibited mRNA expression of apoptosis-related molecules in p38 MAPK signal pathway to downregulate colonic epithelial cells apoptosis in colonic mucosa in mice with colitis.

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Section: Discussionsupporting

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Si Shen Wan Inhibits mRNA Expression of Apoptosis-Related Molecules in p38 MAPK Signal Pathway in Mice with Colitis

Zhao

Huang

Zhou

et al. 2013

Evidence-Based Complementary and Alternative Medicine

Self Cite

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“…Different researchers have modified the procedure by varying the dose and volume of TNBS solution along with change in alcohol concentration in order to induce IBD of varying degree in Sprague Dawley or Wistar rats of either sex. These include intracolonic administration of 0.8 ml of 5% TNBS in 50% (v/v) ethanol [46]; 1 ml of 3% TNBS in 40% ethanol [36] and 0.5 ml of TNBS (100 mg/kg) [47]. Additionally, Qin et al compared the influence of variation in TNBS dose, ethanol concentration, depth of the site of instillation in TNBS induces PI-IBS.…”

Section: Chemical Induced Models Of Chronic Intestinal Inflammationmentioning

confidence: 99%

A Review on Chemical-Induced Inflammatory Bowel Disease Models in Rodents

Randhawa

Singh

et al. 2014

Korean J Physiol Pharmacol

357

310

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Ulcerative colitis and Crohn's disease are a set of chronic, idiopathic, immunological and relapsing inflammatory disorders of the gastrointestinal tract referred to as inflammatory bowel disorder (IBD). Although the etiological factors involved in the perpetuation of IBD remain uncertain, development of various animal models provides new insights to unveil the onset and the progression of IBD. Various chemical-induced colitis models are widely used on laboratory scale. Furthermore, these models closely mimic morphological, histopathological and symptomatical features of human IBD. Among the chemical-induced colitis models, trinitrobenzene sulfonic acid (TNBS)-induced colitis, oxazolone induced-colitis and dextran sulphate sodium (DSS)-induced colitis models are most widely used. TNBS elicits Th-1 driven immune response, whereas oxazolone predominantly exhibits immune response of Th-2 phenotype. DSS-induced colitis model also induces changes in Th-1/Th-2 cytokine profile. The present review discusses the methodology and rationale of using various chemical-induced colitis models for evaluating the pathogenesis of IBD.

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Poly‐pharmacokinetic strategy represented the synergy effects of bioactive compounds in a traditional Chinese medicine formula, Si Shen Wan and its separated recipes to normal and colitis rats

Liu

Wang

et al. 2021

J of Separation Science

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is a classic traditional Chinese medicine formula, which has been used to treat chronic colitis for thousands of years. Many research and experience show that Si Shen Wan was developed by the combination of two sets of "Herb Pairs," Er Shen Wan and Fructus Schisandrae Chinensis Powder. This research aimed to revealing the effective substances, guide the clinical treatment, and represent the synergy effects from the view of pharmacokinetics. An ultra high performance liquid chromatography with tandem mass spectrometry method was established and validated for simultaneous quantification of 26 main bioactive compounds in normal and colitis rat plasma after oral administration of Si Shen Wan and its "Herb Pairs" extract. The method validation results illustrated that the experimental method was reliable and reproducible for quantitative determination of the biological samples. The pharmacokinetic behaviors in different groups were compared and discussed comprehensively, which indicated that the treatment of Si Shen Wan has a superiority in synthetic action of the "Herb Pairs" for the higher peak concentrations and bioavailability of some mainly components. Furthermore, the synergy effect was still existing backed up again for the longer eliminate time and a better bioavailability in colitis groups. The pharmacokinetics research of multiple components in Si Shen Wan and its "Herb Pairs" supplied a significant basis for better understanding the metabolic mechanism of these formulas in both normal and pathological state.

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The protective and healing effects of Si Shen Wan in trinitrobenzene sulphonic acid-induced colitis

Cited by 25 publications

References 29 publications

Si Shen Wan Inhibits mRNA Expression of Apoptosis-Related Molecules in p38 MAPK Signal Pathway in Mice with Colitis

Si Shen Wan Inhibits mRNA Expression of Apoptosis-Related Molecules in p38 MAPK Signal Pathway in Mice with Colitis

A Review on Chemical-Induced Inflammatory Bowel Disease Models in Rodents

Poly‐pharmacokinetic strategy represented the synergy effects of bioactive compounds in a traditional Chinese medicine formula, Si Shen Wan and its separated recipes to normal and colitis rats

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