The protective activity of imperatorin in cultured neural cells exposed to hypoxia re-oxygenation injury via anti-apoptosis

Wang, Nan; Wu, Lei; Cao, Yanjun; Wang, Yuanji; Zhang, Yanmin

doi:10.1016/j.fitote.2013.07.007

Cited by 43 publications

(33 citation statements)

References 29 publications

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“…Process network analysis revealed that apoptotic and anti-apoptotic pathways existed in both 3h and 12h groups, consistent with the findings from a previous study [49]. Our study also found that apoptosis existed in the top 10 processes at 24 h, whereas anti-apoptotic process was absent.…”

Section: Discussionsupporting

confidence: 92%

Time-dependent variation of pathways and networks in a 24-hour window after cerebral ischemia-reperfusion injury

Wang

et al. 2015

BMC Syst Biol

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BackgroundCerebral ischemia-reperfusion injury may simultaneously result in functional variation of multiple genes/pathways. However, most prior time-sequence studies on its pathomechanism only focused on a single gene or pathway. Our study aimed to systematically analyze the time-dependent variation in the expression of multiple pathways and networks within 24 h after cerebral ischemia-reperfusion injury.ResultsBy uploading 374 ischemia-related genes into the MetaCore software, the variation in the expression of multiple pathways and networks in 3 h, 12 h, and 24 h after cerebral ischemia-reperfusion injury had been analyzed. The conserved TNFR1-signaling pathway, among the top 10 pathways, was consistently enriched in 3 h, 12 h, and 24 h groups. Three overlapping pathways were found between 3 h and 12 h groups; 2 between 12 h and 24 h groups; and 1 between 3 h and 24 h groups. Five, 4, and 6 non-overlapping pathways were observed in 3 h, 12 h, and 24 h groups, respectively. Apart from pathways reported by earlier studies, we identified a novel pathway related to the time-dependent development of cerebral ischemia pathogenesis. The process of apoptosis stimulation by external signals, among the top 10 processes, was consistently enriched in 3 h, 12 h, and 24 h groups; 2, 1, and 2 processes overlapped between 3 h and 12 h groups, 12 h and 24 h groups, and 3 h and 24 h groups, respectively. Four, 5, and 5 non-overlapping processes were found in 3 h, 12 h and 24 h groups, respectively. The presence of apoptotic processes was observed in all the 3 groups; while anti-apoptotic processes only existed in 3 h and 12 h groups. Additionally, according to node degree, network comparison identified 1, 8,and 5 important genes or proteins (e.g. Pyk2, PKC, E2F1, and VEGF-A) in 3 h, 12 h, and 24 h groups, respectively. The Jaccard similarity index revealed a higher level of similarity between 12 h and 24 h groups than that between 3 h and 12 h groups.ConclusionTime-dependent treatment can be utilized to reduce apoptosis, which may activate anti-apoptotic pathways within 12 h after cerebral ischemia-reperfusion injury. Pathway and network analyses may help identify novel pathways and genes implicated in disease pathogenesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12918-015-0152-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Time-dependent variation of pathways and networks in a 24-hour window after cerebral ischemia-reperfusion injury

Wang

et al. 2015

BMC Syst Biol

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“…BDNF can protect different types of neurons from ischemic, traumatic and toxic brain injury (Weishaupt et al 2012). BDNF can also inhibit caspase-3 activation and cell apoptosis caused by hypoxia-ischemic injury in vivo (Wang et al 2013b). Moreover, BDNF affects the apoptotic processes of Raji cells, especially Bcl-2 and caspase-signalling pathways.…”

Section: Discussionmentioning

confidence: 99%

SMND-309 promotes neuron survival through the activation of the PI3K/Akt/CREB-signalling pathway

Wang

Zhang

Han

et al. 2016

Pharmaceutical Biology

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Context In clinical practice, the promotion of neuron survival is necessary to recover neurological functions after the onset of stroke. Objective This study aimed to investigate the post-ischaemic neuroprotective effect of SMND-309, a novel metabolite of salvianolic acid, on differentiated SH-SY5Y cells. Materials and methods SH-SY5Y cells were differentiated by pre-treating with 5 mM all-transretinoic acid for 6 d. The differentiated SH-SY5Y cells were exposed to oxygen-glucose deprivation (OGD) for 2 h and reperfusion (R) for 24 h to induce OGD/R injury. After OGD injury, differentiated SH-SY5Y cells were treated with or without SMND-309 (5, 10, 20 mM) for another 24 h. Cell viability was detected through Cell counting kit-8 assay and lactate dehydrogenase leakage assay. Apoptosis was evaluated through flow cytometry, caspase-3 activity assay. Changes in protein levels were assessed through Western blot. Results SMND-309 ameliorated the degree of injury in the differentiated SH-SY5Y cells by increasing cell viabilities (5 mM, 65.4% ± 4.1%; 10 mM, 69.8% ± 3.7%; 20 mM, 75.3% ± 5.1%) and by reducing LDH activity (20 mM, 2.5 fold) upon OGD/R stimulation. Annexin V-fluorescein isothiocyanate/propidium iodide staining results suggested that apoptotic rate of differentiated SH-SY5Y cells decreased from 43.8% induced by OGD/R injury to 19.2% when the cells were treated with 20 mM SMND-309. SMND-309 significantly increased the Bcl-2 level of the injured differentiated SH-SY5Y cells but decreased the caspase-3 activity of these cells by 1.6-fold. In contrast, SMND-309 did not affect the Bax level of these cells. SMND-309 evidently increased the protein expression of BDNF when Akt and CREB were activated. This function was antagonized by the addition of LY294002. Conclusion SMND-309 can prevent neuronal cell death in vitro. This process may be related to the activation of the PI3K/Akt/CREB-signalling pathway.ARTICLE HISTORY

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“…Among them, coumarins were considered as the dominant chemical constituents. It was reported that coumarins possessed various pharmacological effects, such as imperatorin, which had the antihypertensive, antioxidant [5], anti-inflammatory [6], anti-cancer [7], anticonvulsant [8], vasodilatation [9], and anti-HIV-1 [10] activities, and the prevention on lipopolysaccharide (LPS)-induced acute lung injury [11], cerebral ischemia [12], cardiac hypertrophy and heart failure [13]. Isoimperatorin was found to have the anti-Mycobacterium tuberculosis [14] and anti-inflammatory [15] activities, as well as prevention on aflatoxin B 1 -inducible cytotoxicity [16].…”

Section: Var Formosana (Boiss) Shan Et Yuan CV Chuanbaizhi (Radfc)mentioning

confidence: 99%

New coumarins from the roots of Angelica dahurica var. formosana cv. Chuanbaizhi and their inhibition on NO production in LPS-activated RAW264.7 cells

et al. 2015

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The protective activity of imperatorin in cultured neural cells exposed to hypoxia re-oxygenation injury via anti-apoptosis

Cited by 43 publications

References 29 publications

Time-dependent variation of pathways and networks in a 24-hour window after cerebral ischemia-reperfusion injury

Time-dependent variation of pathways and networks in a 24-hour window after cerebral ischemia-reperfusion injury

SMND-309 promotes neuron survival through the activation of the PI3K/Akt/CREB-signalling pathway

New coumarins from the roots of Angelica dahurica var. formosana cv. Chuanbaizhi and their inhibition on NO production in LPS-activated RAW264.7 cells

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