The protection effects of different dosages of ulinastatin on myocardial

Abstract

The aim is to explore the protection effect of ulinastatin (UTI) on myocardial in different dosages and its mechanism. From May to August 2014, in the animal laboratory of the 309th hospital of PLA, the experiment was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals for the National Institutes of Health. The animal protocol was approved by the Institutional Animal Care Committee of the 309th Hospital of PLA (Permit Number: 13-269). 120 SD rats were randomly divided into control group (treated with normal saline), UTI low-dose group (30000 IU/kg), UTI moderate-dose group (60000 IU/kg) and high-dose group (90000 IU/kg). All rats were treated with UTI for 2 weeks via vein. Then left ventricular end diastolic diameter (LVEDD), left ventricular endsystolic dimension (LVESD) and left ventricular ejection fraction (LVEF) were employed to evaluate heart function. Hyp determination with chloramine-T oxidation method was employed to detect the myocardial fibrosis degree, while brain natriuretic peptide (BNP) was used to assess heart function. By comparison among groups, the results of the high-dose group were better than that of any other group in LVEDD, LVESD, LVEF, BNP content, as well as in heart function.It is better for myocardial protection with high-dose of ulinastatin, the possible mechanism is relevant to inhibit myocardial interstitial fibrosis and ventricular remodeling.