The Prospects of Human CD40L-Activated Antigen-Presenting B Cells

Abstract

B cells are considered as professional antigen presenting cells (APCs) and being used to induce the activation of cognate T cells. Because the number of primary APC from individuals' blood are limited, in vitro activated and expanded B cells represent a good alternative APC source for studying T cell responses against pathogen or cancer. With the discoveries of T-B collaboration and T cell help for B cell activation, several groups have successfully established CD40L-based methods for generating antigen-presenting B cells. This review aims to overview current representative CD40L-based B cell culture methods with their concomitant characteristics, applications, and significances.

Monoclonal antibodies targeting nonstructural viral antigens can activate ADCC against human cytomegalovirus

“…UL141-specific B cells were more numerous than UL16-specific B cells ( Figure 3A ). Single antigen-specific B cells were then flow-sorted into culture medium containing CD40L + feeders, IL-2, IL-4, IL-21, and B cell activating factor (BAFF) to generate plasma cells ( 51 ). All secreted mAbs were then screened against cells expressing UL16 or UL141.…”