The Prospects for Using Bioluminescent Enzymatic Analysis to Reveal the Exercise Capacity of Sports Horses

Abstract

Bioluminescent testing of saliva of sports horses, selected before and after physical exercise, was carried out using a coupled enzyme reaction catalyzed by NADH:FMN-oxidoreductase and bacterial luciferase. The inhibitory effect of saliva on the intensity of the light emission was shown, which depended on an increase in the pH of saliva after muscle work. A decrease in the integral bioluminescent index after low-intensity muscular work correlated with the content of total protein and glucose in the blood an serum increase in the bioluminescent index at high intensity was due to a decrease in creatinine or an increase in aspartate aminotransferase and is corelated with an increase in heart rate. It has been shown for the first time that the inhibition of bioluminescence can be an indicator of the functional state of a horse in training, which can be used in sports horse breeding to prevent overloads.