The properties of trehalase from the mosquito-parasitizing water mold, Lagenidium sp

McInnis, T. M.; Domnas, A.

doi:10.1016/0022-2011(73)90157-2

Cited by 20 publications

(9 citation statements)

References 23 publications

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“…Trehalose is one of the most abundant carbohydrates in insect haemocoel (Humber ). The insect‐pathogenic oomycete Lagenidium giganteum , for example, utilizes trehalose in the haemocoel more efficiently than its mosquito host (McInnis & Domnas ). The hypocrealean insect‐pathogenic fungus Metarhizium acridum requires the gene ATM1, which encodes an extracellular acid trehalase to obtain trehalose, and ΔATM1 mutants have reduced virulence towards the host Locusta migratoria (Zhao et al .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomics reveal host‐specific nucleotide variation in entomophthoralean fungi

2016

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Obligate parasites are under strong selection to increase exploitation of their host to survive while evading detection by host immune defences. This has often led to elaborate pathogen adaptations and extreme host specificity. Specialization on one host, however, often incurs a trade-off influencing the capacity to infect alternate hosts. Here, we investigate host adaptation in two morphologically indistinguishable and closely related obligate specialist insect-pathogenic fungi from the phylum Entomophthoromycota, Entomophthora muscae sensu stricto and E. muscae sensu lato, pathogens of houseflies (Musca domestica) and cabbage flies (Delia radicum), respectively. We compared single nucleotide polymorphisms within and between these two E. muscae species using 12 RNA-seq transcriptomes from five biological samples. All five isolates contained intra-isolate polymorphisms that segregate in 50:50 ratios, indicative of genetic duplication events or functional diploidy. Comparative analysis of dN/dS ratios between the multinucleate E. muscae s.str. and E. muscae s.l. revealed molecular signatures of positive selection in transcripts related to utilization of host lipids and the potential secretion of toxins that interfere with the host immune response. Phylogenetic comparison with the nonobligate generalist insect-pathogenic fungus Conidiobolus coronatus revealed a gene-family expansion of trehalase enzymes in E. muscae. The main sugar in insect haemolymph is trehalose, and efficient sugar utilization was probably important for the evolutionary transition to obligate insect pathogenicity in E. muscae. These results support the hypothesis that genetically based host specialization in specialist pathogens evolves in response to the challenge of using resources and dealing with the immune system of different hosts.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptomics reveal host‐specific nucleotide variation in entomophthoralean fungi

2016

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“…In contrast, enzyme preparations from culminating D. discoideum exist as both soluble and insoluble forms, similar to the trehalases from the water mould, Lagenidium sp. (McInnis & Domnas, 1973) and the harvester termite, Trineruitermes trineruoides (Retief & Hewitt, 1973). On the other hand, most of the trehalase activity in crude homogenates from the honey bee (Lefebre & Huber, 1970), rat intestine (Sacktor, 1968) and rabbit kidney (Sasajma et al, 1975) is particulate, with little enzyme activity being soluble.…”

Section: Discussionmentioning

confidence: 99%

Alterations in Trehalase Solubility during Development in the Cellular Slime Mould Dictyostelium discoideum

Killick¹

1985

Microbiology

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Previous studies have indicated that during development in the slime mould Dictyostelium discoideum, compartmentation of the isoenzymes of trehalase (alpha, alpha'-trehalose 1-D-glucohydrolase, (EC 3.2.1.28) occurs between the extracellular and intracellular environments. The compartmentation of trehalase between soluble and particulate cell fractions was examined in this work. The trehalase present in crude homogenates prepared during the first 12 h of development was completely soluble. Starting at about the pseudoplasmodial stage (i.e. the 14th hour of development), trehalase activity became associated with insoluble cellular material and this increased to a maximal value in homogenates from mature sorocarps, where 50% of the activity was insoluble. Spore cells accounted for only 2 to 3% of the trehalase associated with mature sorocarps, with the remaining 97% being localized in stalk cell material. Although trehalase recovered from spores was completely soluble, more than half of that from the stalk was recovered in the buffer-insoluble pellet fraction.

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“…Various monosaccharides, oligosaccharides Lagenidium sp. (McInnis and Domnas, 1973) but It is probably involved in breakdown of trehalose from its insect host.…”

Section: Carbohydrate Reservesmentioning

confidence: 99%

Intermediary Metabolism

Laere¹

1995

The Growing Fungus

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The properties of trehalase from the mosquito-parasitizing water mold, Lagenidium sp

Cited by 20 publications

References 23 publications

Comparative transcriptomics reveal host‐specific nucleotide variation in entomophthoralean fungi

Comparative transcriptomics reveal host‐specific nucleotide variation in entomophthoralean fungi

Alterations in Trehalase Solubility during Development in the Cellular Slime Mould Dictyostelium discoideum

Intermediary Metabolism

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