The Properties of the Multistage Bioreactor Constructed by the LB Technique

Abstract

A multistage bioreactor consisting of organic ultrathin layers was successfully realized by immobilizing glucose oxidase and glucoamylase by the Langmuir–Blodgett technique (LB technique). Starch as the substrate was quickly converted into gluconic acid and hydrogen peroxide as the products in this bioreactor via the intermediate, glucose. The rate of the sequential reactions was electrochemically measured. This rate increased proportionally with increasing the concentration of starch, and also increased with increasing the amount of glucose oxidase in the LB film. This bioreactor, however, decayed soon. The reason of this decay was considered to be the structural conversion of the LB layers with glucoamylase. The multistage bioreactor constructed by the LB technique will be used not only as a super small-sized, rapid bioreactor or biosensor but also as an investigating means of the reconstitution of membrane enzyme and biomembrane.

Surface density as a significant parameter for the enzymatic activity of two forms of alkaline phosphatase immobilized on phospholipid Langmuir–Blodgett films

“…Immobilization of enzymes on solid supports is important for the development of biosensors and bioreactors [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. The solid support and the enzyme immobilization should avoid enzyme denaturation and must also provide an orientation and enzymatic surface density that maximize the catalytic activity.…”

“…The adsorption of proteins onto Langmuir and LB lipid films has been widely used to study molecular interactions with drugs, molecular recognition, and other relevant biophysical phenomena [1][2][3][4][5][6][7][8][9][10][11][21][22][23]. Particularly, alkaline phosphatases from different sources have been studied at air/buffer interfaces and immobilized in thin films deposited on solid substrates by several research groups [24][25][26][27][28][29][30][31].…”

A Review on Sweet Potato Syrup Production Process: Effective Parameters and Syrup Properties

“…Historically, several engineering methods [112][113][114][115][116][117][118] have been used to enhance the starch hydrolysis process. These methods may include the use of fluidized-bed reactors and fixed-bed reactors among others.…”