The properties of lipid bilayer membranes separating two aqueous phases: Formation of a membrane of simple composition

Huang, Chunbo; Wheeldon, L. W.; Thompson, T. E.

doi:10.1016/s0022-2836(64)80155-8

Cited by 137 publications

(41 citation statements)

References 24 publications

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“…Membrane stability, DC resistance, and capacitance (14) were independent of temperature over the range 21-38°C. These properties were also independent (within the range of precision permitted by our methods) of pH (5.1-7.2) and the kind (NaC1, KC1, Tris C1) and concentration (0.005-0.25 M) of electrolyte in the aqueous phase (10,14). Most experiments were performed in unbuffered 0.01 or 0.1 u NaC1 or KCI (pH ~5,6) to simplify calculation of ionic transference numbers.…”

Section: Properties Of the Membranementioning

confidence: 84%

“…Since these membranes are analogous to bimoleeular black soap fdms formed in air (8), they provide an interesting synthetic model of the bimolecular lipid leaflet originally proposed by Davson and Danielli (9) as the fundamental unit of biological membranes. Subsequently, Huang et al (10) formed similar membranes from purified lecithin and an aliphatic hydrocarbon in chloroform methanol. Using optical methods (10,11), they computed membrane thickness to be 61 A and, more recently, have described the water permeability properties of these membranes (12).…”

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confidence: 99%

“…Subsequently, Huang et al (10) formed similar membranes from purified lecithin and an aliphatic hydrocarbon in chloroform methanol. Using optical methods (10,11), they computed membrane thickness to be 61 A and, more recently, have described the water permeability properties of these membranes (12). Schwan et al (13) have calculated the capacitance of these membranes to be 0.6-0.7 uf/cm 2.…”

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confidence: 99%

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The Formation and Properties of Thin Lipid Membranes from HK and LK Sheep Red Cell Lipids

Andreoli

Bangham

Tosteson

1967

The Journal of General Physiology

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Lipids were obtained from high potassium (HK) and low potassium (LK) sheep red cells by sequential extraction of the erythrocytes with isopropanol-chloroform, chloroform-methanol-0.1 ~ KC1, and chloroform. The extract contained cholesterol and phospholipid in a molar ratio of 0.8 : 1.0, and less than 1% protein contaminant. Stable thin lipid membranes separating two aqueous compartments were formed from an erythrocyte lipid-hydrocarbon solution, and had an electrical resistance of ~-~108 ohm-cm ~ and a capacitance of 0.38-0.4 ~f/cm ~. From the capacitance values, membrane thickness was estimated to be 46-132 A, depending on the assumed value for the dielectric constant (2.0-4.5). Membrane voltage was recorded in the presence of ionic (NaCl and/or KC1) concentration gradients in the solutions bathing the membrane. The permeability of the membrane to Na +, K +, and C1-(expressed as the transference number, Tion) was computed from the steady-state membrane voltage and the activity ratio of the ions in the compartments bathing the membrane. TNa and TK were approximately equal (~-~0.8) and considerably greater than Tel (,~0.2). The ionic transference numbers were independent of temperature, the hydrocarbon solvent, the osmolarity of the solutions bathing the membranes, and the cholesterol content of the membranes, over the range 21-38°C. The high degree of membrane cation selectivity was tentatively attributed to the negatively charged phospholipids (phosphatidylethanolamine and phosphatidylserine) present in the lipid extract. I N T R O D U C T I O NThe relative permeabilities of high-potassium (HK) and low-potassium (LK) sheep red cell membranes to sodium and potassium ions are quite different (1, 2). The difference in cation selectivity may be a consequence of the physicochemical properties of the membrane lipids in the two genetically determined cell types. In order to explore this possibility, the ionic permeability properties of artificial membranes prepared from H K and L K sheep erythrocyte lipids were characterized.

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Section: Properties Of the Membranementioning

confidence: 84%

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confidence: 99%

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confidence: 99%

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The Formation and Properties of Thin Lipid Membranes from HK and LK Sheep Red Cell Lipids

Andreoli

Bangham

Tosteson

1967

The Journal of General Physiology

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“…The results are expressed in this manner for convenience. Moreover, it should be noted that biological membranes generally cannot support potentials in excess of 150 to 200 mV, see [26][27][28][29][30].…”

Section: Resultsmentioning

confidence: 99%

Phosphorylation without protonmotive force

Kinnally¹,

Tedeschi²

1976

FEBS Letters

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“…EggPC is a mixture of lipids whose acyl-chains are about 50% saturated and 50% unsaturated (Huang et al, 1964). The fatty acyl chains have been identified to include palmitic, stearic, oleic, and linoleic acids.…”

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Photoinduced Fusion of Liposomes

Bennett¹,

O’Brien²

1994

J. Am. Chem. Soc.

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This manuscript ,has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissenation copies are in typewriter face, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note wiD indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continning from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.

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The properties of lipid bilayer membranes separating two aqueous phases: Formation of a membrane of simple composition

Cited by 137 publications

References 24 publications

The Formation and Properties of Thin Lipid Membranes from HK and LK Sheep Red Cell Lipids

The Formation and Properties of Thin Lipid Membranes from HK and LK Sheep Red Cell Lipids

Phosphorylation without protonmotive force

Photoinduced Fusion of Liposomes

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