The properties of bioengineered chondrocyte sheets for cartilage regeneration

Mitani, Genya; Sato, Masato; Lee, Jeong Ki; Kaneshiro, Nagatoshi; Ishihara, Miya; Ota, Naoshi; Kokubo, Mami; Sakai, Hideaki; Kikuchi, Tetsutaro; Mochida, Joji

doi:10.1186/1472-6750-9-17

Cited by 76 publications

(73 citation statements)

References 24 publications

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“…Mitani et al investigated the properties of chondrocyte sheets and showed that expression of Sox 9, collagen type 2 and 27, integrin a10, and fibronectin were higher in layered chondrocyte sheets than in a single chondrocyte sheet in vitro. 21 In addition, Hamahashi et al showed that layered chondrocyte sheets produced higher levels of TGFb and PGE2 as humoral factors compared with monolayer chondrocyte sheets and suspected that these factors might contribute to cartilage repair. 22 However, these previously reported results might not…”

Section: Discussionmentioning

confidence: 99%

Repair Mechanism of Osteochondral Defect Promoted by Bioengineered Chondrocyte Sheet

Shimizu

Kamei

Adachi

et al. 2015

Tissue Engineering Part A

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Cell sheet engineering has developed as a remarkable method for cell transplantation. In the field of cartilage regeneration, several studies previously reported that cartilage defects could be regenerated by transplantation of a chondrocyte sheet using cell sheet engineering. However, it remains unclear how such a thin cell sheet could repair a deep cartilage defect. We, therefore, focused on the mechanism of cartilage repair using cell sheet engineering in this study. Chondrocyte sheets and synovial cell sheets were fabricated using cell sheet engineering, and these allogenic cell sheets were transplanted to cover an osteochondral defect in a rat model. Macroscopic and histological evaluation was performed at 4 and 12 weeks after transplantation. Analysis of the gene expression of each cell sheet and of the regenerated tissue at 1 week after transplantation was performed. In addition, green fluorescent protein (GFP) transgenic rats were used as donors (transplanted chondrocyte sheets) or recipients (osteochondral defect models) to identify the cell origin of regenerated cartilage.Cartilage repair was significantly better in the group implanted with a chondrocyte sheet than in that with a synovial cell sheet. The results of gene expression analysis suggest that the possible factor contributing to cartilage repair might be TGFb1. Cell tracking experiments using GFP transgenic rats showed that the regenerated cartilage was largely composed of cells derived from the transplanted chondrocyte sheets.

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Section: Discussionmentioning

confidence: 99%

Repair Mechanism of Osteochondral Defect Promoted by Bioengineered Chondrocyte Sheet

Shimizu

Kamei

Adachi

et al. 2015

Tissue Engineering Part A

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“…However, this study did not discuss the related indexes of the scaffold. In subsequent studies, we will assess new scaffold materials, electrostatic spinning (Agarwal et al, 2008;Janjanin et al, 2008;Wright et al, 2010), and cell-chip technology (Kaneshiro et al, 2006;Mitani et al, 2009;Tani et al, 2010;Weder et al, 2010), and in more detail compare the properties of the engineered cartilages with normal cartilages.…”

Section: Discussionmentioning

confidence: 99%

Repair of cartilage defects in BMSCs via CDMP1 gene transfection

Wu¹,

Cui²,

Wang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study aimed at investigating the ability of cartilagederived morphogenetic protein 1 (CDMP1) gene-transfected bone marrow mesenchymal stem cells (BMSCs) loaded on the poly(lacticco-glycolic acid) (PLGA) scaffold for the repair of laryngeal cartilage defects and make a preliminary assessment of its repair effect. The mRNA and protein expressions of hCDMP1 were detected by reverse transcriptase-polymerase chain reaction and Western blotting. The expression of type II collagen (Col II) and glycosaminoglycan (GAG) were detected by immunohistochemistry. The cytoskeletal culture systems before and after transfection were transplanted into the rabbit full-thickness defects of thyroid cartilage for observation of the repair of cartilage defects from general and histological aspects. The exogenous hCDMP1 gene could be successfully transplanted into BMSCs through adenovirus infection to obtain a stable expression. Compared with the control group, hCDMP1 gene-transfected BMSCs had enhanced secretory abilities of Col II, GAG, and other cartilagespecific matrices, with a trend of promoting cartilage differentiation. The transfected cytoskeletal complexes could more effectively repair laryngeal cartilage defects. hCDMP1 gene-transfected BMSCs/PLGA 3-D biological scaffold compounds transplanted into animal bodies could effectively repair laryngeal cartilage defects.

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“…In fact, piezoelectric materials are being widely adapted for use as scaffolds for tissue regeneration and repair applications and have even been shown to influence gene expression in the case of cartilaginous implants utilizing the piezoelectric polymer polyvinylidene fluoride (PVDF). 49,50 Fibroblasts grown on piezoelectrically-activated (presenting charge on surface) implants show enhanced migration, adhesion and secretion and the implants themselves showed higher fibrosis levels in rats in vivo, illustrating wound healing capabilities. 51 However, in order to elucidate the fundamental basis for the observed improved cellular function due to charge, synthetic biology techniques can be employed.…”

Section: Effect Of Substrate Stiffnessmentioning

confidence: 99%

Elucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approaches

Denning

Roos

2016

Cell Adhesion & Migration

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The use of synthetic surfaces and materials to influence and study cell behavior has vastly progressed our understanding of the underlying molecular mechanisms involved in cellular response to physicochemical and biophysical cues. Reconstituting cytoskeletal proteins and interfacing them with a defined microenvironment has also garnered deep insight into the engineering mechanisms existing within the cell. This review presents recent experimental findings on the influence of several parameters of the extracellular environment on cell behavior and fate, such as substrate topography, stiffness, chemistry and charge. In addition, the use of synthetic environments to measure physical properties of the reconstituted cytoskeleton and their interaction with intracellular proteins such as molecular motors is discussed, which is relevant for understanding cell migration, division and structural integrity, as well as intracellular transport. Insight is provided regarding the next steps to be taken in this interdisciplinary field, in order to achieve the global aim of artificially directing cellular response.

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The properties of bioengineered chondrocyte sheets for cartilage regeneration

Cited by 76 publications

References 24 publications

Repair Mechanism of Osteochondral Defect Promoted by Bioengineered Chondrocyte Sheet

Repair Mechanism of Osteochondral Defect Promoted by Bioengineered Chondrocyte Sheet

Repair of cartilage defects in BMSCs via CDMP1 gene transfection

Elucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approaches

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