The promyelinating properties of androstenediol in gliotoxin‐induced demyelination in rat corpus callosum

Kalakh, Samah; Mouihate, Abdeslam

doi:10.1111/nan.12237

Cited by 15 publications

(34 citation statements)

References 61 publications

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“…Gliotoxin‐induced demyelination is characterized by a local brain inflammatory response. This inflammatory response is largely mediated by a localized activation of microglial cells, the immune‐competent cells within the brain . Therefore, we explored whether prenatal exposure to LPS alters demyelination‐induced activation of microglia.…”

Section: Resultsmentioning

confidence: 99%

“…Adult male offspring (2‐3 months) received a stereotaxic injection of 2 μL of 0.04% ethidium bromide (EB) solution (Sigma Chemical Co., St. Louis, MO, USA) into the corpus callosum using the following stereotaxic coordinates (bregma: 0 mm, lateral: 2.5 mm, dorsoventral: 3.4 mm) as previously described . The corpus callosum lesion was verified using Luxol fast blue (LFB) staining as detailed in our previous studies . The rats with lesion outside of the corpus callosum were discarded from this study.…”

Section: Methodsmentioning

confidence: 99%

“…The rats with lesion outside of the corpus callosum were discarded from this study. The animals were sacrificed 7 days postdemyelination insult because this time point represents the peak of demyelination injury following EB injection …”

Section: Methodsmentioning

confidence: 99%

“…Astrocytosis was monitored by measuring the integrated optical density (OD) of GFAP‐containing cells using the 63× objective. Alternatively, GFAP immunoreactivity was binarized using the ImageJ software and the percentage of area covered by GFAP‐containing cells was used as an index of astrogliosis as previously described …”

Section: Methodsmentioning

confidence: 99%

“…29,30 The corpus callosum lesion was verified using Luxol fast blue (LFB) staining as detailed in our previous studies. 29,30 The rats with lesion outside of the corpus callosum were discarded from this study. The animals were sacrificed 7 days postdemyelination insult because this time point represents the peak of demyelination injury following EB injection.…”

Section: Demyelination Lesionmentioning

confidence: 99%

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Impact of prenatal immune challenge on the demyelination injury during adulthood

et al. 2017

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Demyelination Lesionmentioning

confidence: 99%

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Impact of prenatal immune challenge on the demyelination injury during adulthood

et al. 2017

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Demyelination-Induced Inflammation Attracts Newly Born Neurons to the White Matter

Kalakh

Mouihate

2016

Mol Neurobiol

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There is compelling evidence that microglial activation negatively impacts neurogenesis. However, microglia have also been shown to promote recruitment of newly born neurons to injured areas of the gray matter. In the present study, we explored whether demyelination-triggered inflammation alters the process of neurogenesis in the white matter. A 2-μl solution of 0.04 % ethidium bromide was stereotaxically injected into the corpus callosum of adult male rats. Brain inflammation was dampened by daily injections of progesterone (5 mg/kg, s.c.) for 14 days. Control rats received oil (s.c.). Newly born neurons (DCX and Tbr2), microglia (Iba-1), astrocytes (vimentin or GFAP), oligodendrocyte progenitor cells (OPCs; NG2), and mature oligodendrocytes (CC-1) were monitored in the vicinity of demyelination site using immunofluorescent staining. Western blot was used to explore microglial polarization using M1 (iNOS) and M2 (arginase-1) markers. Focal demyelination elicited strong microglial and astroglial activation and reduced the number of OPCs at the site of demyelination. This inflammatory response was associated with enhanced number of newly born neurons in the white matter and the subventricular zone (SVZ). A proportion of newly born neurons within the white matter showed features of OPCs. Interestingly, blunting brain inflammation led to reduced neurogenesis around the demyelination area and in the SVZ. These data suggest that the white matter inflammation creates a conducive environment for the recruitment of newly born neurons. The fact that a sizable fraction of these newly born neurons adopt OPC features suggests that they could contribute to the remyelination process.

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PPAR-γ Is Critical for HDAC3-Mediated Control of Oligodendrocyte Progenitor Cell Proliferation and Differentiation after Focal Demyelination

Ding

Zhou

et al. 2020

Mol Neurobiol

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The promyelinating properties of androstenediol in gliotoxin‐induced demyelination in rat corpus callosum

Abstract: These data strongly suggest that androstenediol is endowed with promyelinating properties in a model of focal gliotoxin-induced demyelination. It induces its promyelinating effects by enhancing the number of oligodendrocyte precursor cells and their maturation at the lesion site.

Cited by 15 publications

References 61 publications

Impact of prenatal immune challenge on the demyelination injury during adulthood

Impact of prenatal immune challenge on the demyelination injury during adulthood

Demyelination-Induced Inflammation Attracts Newly Born Neurons to the White Matter

PPAR-γ Is Critical for HDAC3-Mediated Control of Oligodendrocyte Progenitor Cell Proliferation and Differentiation after Focal Demyelination

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