The promotion of oriented axonal regrowth in the injured spinal cord by alginate-based anisotropic capillary hydrogels

Prang, Peter; Müller, Rainer; Eljaouhari, Ahmed A.; Heckmann, Klaus; Kunz, Werner; Weber, Thomas; Faber, Cornelius; Vroemen, Maurice; Bogdahn, Ulrich; Weidner, Norbert

doi:10.1016/j.biomaterials.2006.01.053

Cited by 215 publications

(268 citation statements)

References 48 publications

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“…Although the matrix organization of Matrigel does appear to support the retention of the injected marrow stem cells as well as vessel growth and Schwann cell infiltration, it may interfere with the natural patterns of those events that support directional paths for outgrowing neurites (Cai et al, 2004). An in vitro/in vivo study using alginate-based hydrogels formed as longitudinal arrays also showed the support of axonal outgrowth, but ECM additives failed to augment the response (Prang et al, 2006). Similarly, magnetically aligned laminin fibrils allow for better axonal growth when in a longitudinal compared with a horizontal orientation (Verdu et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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The early events associated with axonal growth into 10-mm nerve gaps were studied histologically in the rat sciatic nerve model to determine if the outgrowth of blood vessels, Schwann cells, and axons could be enhanced. In the first two experimental groups, collagen nerve guides were filled with either saline or Matrigel. Marrow-derived mesenchymal stem cells (MSCs) were added to Matrigel in two other groups, one of which contained cells transfected with VEGF (MSC/VEGF). After 21 days, the injury site was exposed, fixed, sectioned, and volume fractions of the conduit contents were determined by point counting. The bioresorbable collagen conduits appropriately guided the axons and vessels in a longitudinal direction. The volume fraction of axons was significantly greater in the group with saline when compared with all three groups with Matrigel. This measure had a significant positive correlation with actual counts of myelinated axons. The blood vessel volume fraction in the Matrigel group decreased compared with the saline group, but was restored in the MSC/VEGF group. All Matrigel groups had comparable cellularity and showed a distribution of residual Matrigel in acellular zones. The saline group, by contrast, sustained a network of delicate fibroblastic processes that compartmentalized the nerve and its natural matrix as it became infiltrated by axons as minifascicles. In conclusion, the reduction of axonal outgrowth in the Matrigel groups, when compared with the saline group, suggests that Matrigel may impede the early regenerative process even when enriched by the addition of MSCs or VEGF-transfected cells.

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Section: Discussionmentioning

confidence: 99%

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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“…In order to mimic the complex in vivo 3D environment several artificial surfaces and scaffolds have been developed, including micro-and nanostructured surfaces [1][2][3][4][5], biomolecular coatings [6], gels [7], and nano-fibrous scaffolds [8][9][10]. Planar electronically active cell seeding surfaces based on conjugated polymers have been used to control the cell adhesion and proliferation of various cell systems [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Nano-fiber scaffold electrodes based on PEDOT for cell stimulation

Bolin

Svennersten

Wang

et al. 2009

Sensors and Actuators B: Chemical

119

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Electronically conductive and electrochemically active 3D-scaffolds based on electrospun poly(ethylene terephthalate) (PET) nano-fibers are reported. Vapour phase polymerization was employed to achieve an uniform and conformal coating of poly(3,4-ethylenedioxythiophone) doped with tosylate (PEDOT:tosylate) on the nano-fibers. The PEDOT coatings had a large impact on the wettability, turning the hydrophobic PET fibers super-hydrophilic. SH-SY5Y neuroblastoma cells were grown on the PEDOT coated fibers.The SH-SY5Y cells adhered well and showed healthy morphology. These electrically active scaffolds were used to induce Ca 2+ signalling in SH-SY5Y neuroblastoma cells.PEDOT:tosylate coated nano-fibers represents a class of 3D host environments that combines excellent adhesion and proliferation for neuronal cells with the possibility to regulate their signalling.

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“…These materials are easily obtained and can be cross-linked to form three dimensional scaffolds. [57][58][59][60][61][62] Alginate scaffolds are formed by calcium cross-linking and can be degraded by calcium chelation, while agarose forms a gel based on its thermodynamic properties above a certain temperature. Both polysaccharides must undergo extensive purification to prevent immune responses after implantation.…”

Section: Natural Materialsmentioning

confidence: 99%

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The promotion of oriented axonal regrowth in the injured spinal cord by alginate-based anisotropic capillary hydrogels

Cited by 215 publications

References 48 publications

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Nano-fiber scaffold electrodes based on PEDOT for cell stimulation

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