The promotion of bone regeneration through positive regulation of angiogenic–osteogenic coupling using microRNA-26a

Li, Yan; Fan, Longkun; Liu, Shiyu; Liu, Wenjia; Zhang, Hao; Zhou, Tao; Wu, Dan; Yang, Ping; Shen, Lijuan; Chen, Ji Hua; Jin, Yan

doi:10.1016/j.biomaterials.2013.03.052

Cited by 194 publications

(150 citation statements)

References 45 publications

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…The use of commercial cationic lipids in biomaterial-based approaches to deliver miRNA therapeutics is exemplified by the widespread use of Lipofectamine 2000 [53][54][55][56] along with Lipofectamine RNAiMAX [57] and SiPORT NeoFx. [58] Generally, cationic lipids complex with the miRNA cassettes through electrostatic interaction, and the overall positive charge of the complex dictates the cellular uptake, although neutrally charged liposomes can also encapsulate the cargo within their core. Complexes formed by encapsulation of the miRNA cargo, as opposed to charge adsorption, are not limited to lipid-based vectors and have also shown effective delivery profiles in RM applications.…”

Section: Nonviral Vectorsmentioning

confidence: 99%

“…[102,106] Whether one of the routes may be better suited for clinical translation remains to be elucidated, although in situ transfecting scaffolds hold greater potential to exist as "off-the-shelf" products. [107] Many distinct scaffold types have been tuned to serve the purpose of miRNA delivery, including several hydrogels, [58,92,106,[108][109][110][111][112][113][114] electrospun fibers, [63,[115][116][117][118] and more prolifically porous or spongy scaffolds. [46,47,50,54,55,96,112,[119][120][121][122][123][124][125][126][127][128][129][130] Before reviewing the details of their applications, summarized in Table 2 and described further in the next section, we will discuss the key characteristics making these materials amenable to miRNA delivery.…”

Section: Scaffolds For Microrna Deliverymentioning

confidence: 99%

“…Certain materials are more applicable for a fabrication technique or a type of formulation. For example, PEG is a synthetic polymer frequently employed in the preparation of hydrogels, such as the commercial HyStem-HP utilized by Li et al to deliver miR-26a mimics, [58] or the PEG-norbornene system for human bone marrow derived (BM)-MSCs nucleofected with a dual miR-148b mimic + miR-489 inhibitor combination. [106] An in situ forming PEG-hydrogel has also been the supporting biomaterial of an UV-triggered "on-demand" miR-20a release system.…”

Section: Scaffolds For Microrna Deliverymentioning

confidence: 99%

“…[58,63,146] Li et al [58] documented the first report in 2013 using BM-MSCs, HyStem-HP hydrogel, and miR-26a mimic. They demonstrated almost full repair of the defect 12 weeks postimplantation and significantly enhanced vascularization confirming that miR-26a had the capacity to regulate endogenous angiogenic-osteogenic coupling for enhanced bone regeneration.…”

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

“…Following 12 weeks implantation, histological assessment showed markedly enhanced bone formation. The final and most eloquent report went a few steps further and delivered HP-PEI-miR-26a polyplexes on a nanofibrous PLLA scaffold without an external cell source in contrast to the earlier reports [58,146] and of great importance, mechanistically identified the bone regeneration target gene for miR-26a for the first time. [63] To further support the spatiotemporal release of the miRNA, the miRNA-loaded polyplexes were also encapsulated in biodegradable PLGA microspheres which were then immobilized onto the scaffolds.…”

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

See 4 more Smart Citations

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Curtin

Castaño

O’Brien

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

The field of "regenerative medicine" (RM) was conceptually developed to aid the body's natural capacity to self-repair, a capacity which is impaired with age and in cases of disease or injury. [1] RM is a vast and multifaceted area of medicine, often microRNA-based therapies are an advantageous strategy with applications in both regenerative medicine (RM) and cancer treatments. microRNAs (miRNAs) are an evolutionary conserved class of small RNA molecules that modulate up to one third of the human nonprotein coding genome. Thus, synthetic miRNA activators and inhibitors hold immense potential to finely balance gene expression and reestablish tissue health. Ongoing industrysponsored clinical trials inspire a new miRNA therapeutics era, but progress largely relies on the development of safe and efficient delivery systems. The emerging application of biomaterial scaffolds for this purpose offers spatiotemporal control and circumvents biological and mechanical barriers that impede successful miRNA delivery. The nascent research in scaffold-mediated miRNA therapies translates know-how learnt from studies in antitumoral and genetic disorders as well as work on plasmid (p)DNA/siRNA delivery to expand the miRNA therapies arena. In this progress report, the state of the art methods of regulating miRNAs are reviewed. Relevant miRNA delivery vectors and scaffold systems applied to-date for RM and cancer treatment applications are discussed, as well as the challenges involved in their design. Overall, this progress report demonstrates the opportunity that exists for the application of miRNA-activated scaffolds in the future of RM and cancer treatments.Regenerative Medicine

show abstract

Section: Nonviral Vectorsmentioning

confidence: 99%

Section: Scaffolds For Microrna Deliverymentioning

confidence: 99%

Section: Scaffolds For Microrna Deliverymentioning

confidence: 99%

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

Section: Osteogenesis and Bone Repairmentioning

confidence: 99%

See 3 more Smart Citations

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Curtin

Castaño

O’Brien

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

Simultaneous Regeneration of Bone and Nerves Through Materials and Architectural Design: Are We There Yet?

Wan

Qin

Shen

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Bilateral communication between bone and nerves is crucial for optimal repair of large bone defects as well as repair of peripheral nerves within the skeleton. However, simultaneous regeneration of bone and nerves is an issue that has long been overlooked in prior literature. Incongruous or even contradictory requirements or regeneration environments for bone and nerves make simultaneous regeneration of multiple tissues challenging. The present review commences with introducing natural crosstalk between bone and nerves, highlighting the rationale for simultaneous regeneration of bone and nerves. These issues will pave the way for the development of inspirational materials design concepts that capitalize on the principles of osteoconduction, osteoinduction, neuroconduction, neuroinduction, and neuroattraction. Potential strategies based on selection of appropriate scaffolds, acellular biological factors and architectural design will be addressed.

show abstract

3D Hybrid Nanofiber Aerogels Combining with Nanoparticles Made of a Biocleavable and Targeting Polycation and MiR‐26a for Bone Repair

Wang

John

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

The healing of large bone defects represents a clinical challenge, often requiring some form of grafting. 3D nanofiber aerogels could be a promising bone graft due to their biomimetic morphology and controlled porous structures and composition. miR-26a has been reported to induce the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) and facilitate bone formation. Introducing miR-26a with a suitable polymeric vector targeting BMSCs could improve and enhance the functions of 3D nanofiber aerogels for bone regeneration. Herein, the comb-shaped polycation (HA-SS-PGEA) is first developed, carrying a targeting component, biocleavable groups, and short ethanolamine (EA)-decorated poly(glycidyl methacrylate) (PGMA) (abbreviated as PGEA) arms as miR-26a delivery vector. Thereafter, the cytotoxicity and transfection efficiency of this polycation and cellular response to miR-26a-incorporated nanoparticles (NPs) are assessed in vitro. HA-SS-PGEA exhibits a stronger ability to transport miR-26a and exert its functions than the gold standard polyethyleneimine (PEI) and low-molecular-weight linear PGEA. The efficacy of HA-SS-PGEA/ miR-26a NPs loaded 3D hybrid nanofiber aerogels showing a positive effect on the cranial bone defect healing is finally examined. Together, the combination of 3D nanofiber aerogels and functional NPs consisting of a biodegradable and targeting polycation and therapeutic miRNA could be a promising approach for bone regeneration.

show abstract

The promotion of bone regeneration through positive regulation of angiogenic–osteogenic coupling using microRNA-26a

Cited by 194 publications

References 45 publications

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

Simultaneous Regeneration of Bone and Nerves Through Materials and Architectural Design: Are We There Yet?

3D Hybrid Nanofiber Aerogels Combining with Nanoparticles Made of a Biocleavable and Targeting Polycation and MiR‐26a for Bone Repair

Contact Info

Product

Resources

About