The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector

Koncz, Csaba; Schell, Jeff

doi:10.1007/bf00331014

Cited by 1,762 publications

(1,127 citation statements)

References 65 publications

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“…A. tumefaciens strain ATHV, a derivative of A. tumefaciens strain C58 (a Ti plasmid cured nopaline strain), harboring plasmid pEHA101 devoid of its kanamycin-resistance gene was kindly provided by P. Eckes, Hoechst AG, Frankfurt. The derivative of the pPCV vector series (Koncz and Schell, 1986) used in this study (see below) contains the ori v and ori T sequences of plasmid RK2 (Thomas, 1981). Therefore, plasmid pCT153.1 (kindly provided by C. Koncz) harboring the replication and maintenance function of plasmid RK2 in trans (Thomas, 1981) and a bacterial kanamycin-resistance gene, was conjugated from E. coil strain HB101 to the recipient Agrobacterium strain.…”

Section: Bacterial Strains and Culturesmentioning

confidence: 99%

Promoter tagging with a promoterless ipt gene leads to cytokinin‐induced phenotypic variability in transgenic tobacco plants: implications of gene dosage effects

Hewelt

Prinsen

Schell³

et al. 1994

The Plant Journal

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107

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Section: Bacterial Strains and Culturesmentioning

confidence: 99%

Promoter tagging with a promoterless ipt gene leads to cytokinin‐induced phenotypic variability in transgenic tobacco plants: implications of gene dosage effects

Hewelt

Prinsen

Schell³

et al. 1994

The Plant Journal

Self Cite

107

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“…AAOs are widely distributed copper-containing enzymes which catalyse the oxidation of ascorbate to 2-dehydroascorbate with the concomitant reduction of molecular oxygen to water. The precise biological function of AAO has not yet been defined although a possible involvement in an alternative redox system, in growth promotion, in disease susceptibility and in cell wall loosening has been postulated (Butt, 1980;Lin and Varner, 1991). The fact that the active centres of these multicopper enzymes are not conserved in the Bpl 0 proteins suggests that the AAO and BplO genes probably derive from a common ancestral gene but code for proteins having different enzymatic functions.…”

Section: Discussionmentioning

confidence: 99%

A Brassica napus gene family which shows sequence similarity to ascorbate oxidase is expressed in developing pollen. Molecular characterization and analysis of promoter activity in transgenic tobacco plants

Albani

Sardana

Robert³

et al. 1992

The Plant Journal

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SummaryThe genomic clone named BplO contains a member of a small pollen-specific gene family of 9. napus. The expression of the BplO gene family is maximal in early binucleate microspores and declines considerably in mature trinucleate pollen. Homologues of the BplO genes are expressed in the pollen of other plant species. The pollen-specific expression of the gene contained in the genomic clone was confirmed in tobacco plants transformed with a chimeric BplO promoter/GUS construct. A promoter fragment of 396 bp is sufficient to direct a strong and correct spatial and temporal expression in transgenic plants. The BplO gene family codes for proteins of 62 kDa showing approximately 30% sequence identity to cucumber and pumpkin ascorbate oxidases (AAOs). However, the AAO active centres are not conserved in the BplO products, suggesting an evolutionary relationship but a different enzymatic activity for these proteins. Expression of a recombinant BplO protein in €. coli inhibits bacterial growth on minimal medium, suggesting the production of an enzymatically active polypeptide in bacteria. No AAO activity could be correlated with the expression of the recombinant protein. Moreover, substances affecting M O activity do not appear to influence the inhibitory activity of the protein produced in bacteria. However, as indicated by the rescue of bacterial growth in the presence of sodium bicarbonate or gaseous COB, the BplO protein activity could be modulated by COP levels.

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“…Agrobacterium tumefaciens strain C58C1 (ref. 61) was transformed by electroporation, and transformants were selected on YEP plates (1% yeast extract, 2% peptone, 1.5% agar) containing 25 mg ml -1 gentamicin and 50 mg ml -1 kanamycin. Transformants were grown overnight in 2 ml liquid YEP media, washed in infiltration solution (10 mM MgCl 2 , 100 mM 4 0 -Hydroxy-3',5'-dimethoxyacetophenone) and used for infiltration using 3-week-old Nicotiana benthamiana plants.…”

Section: Yeast Microsomal Membrane Preparation and Immunodetectionmentioning

confidence: 99%

A phospholipid uptake system in the model plant Arabidopsis thaliana

et al. 2015

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Plants use solar energy to produce lipids directly from inorganic elements and are not thought to require molecular systems for lipid uptake from the environment. Here we show that Arabidopsis thaliana Aminophospholipid ATPase10 (ALA10) is a P4-type ATPase flippase that internalizes exogenous phospholipids across the plasma membrane, after which they are rapidly metabolized. ALA10 expression and phospholipid uptake are high in the epidermal cells of the root tip and in guard cells, the latter of which regulate the size of stomatal apertures to modulate gas exchange. ALA10-knockout mutants exhibit reduced phospholipid uptake at the root tips and guard cells and are affected in growth and transpiration. The presence of a phospholipid uptake system in plants is surprising. Our results suggest that one possible physiological role of this system is to internalize lysophosphatidylcholine, a signalling lipid involved in root development and stomatal control.

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The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector

Cited by 1,762 publications

References 65 publications

Promoter tagging with a promoterless ipt gene leads to cytokinin‐induced phenotypic variability in transgenic tobacco plants: implications of gene dosage effects

Promoter tagging with a promoterless ipt gene leads to cytokinin‐induced phenotypic variability in transgenic tobacco plants: implications of gene dosage effects

A Brassica napus gene family which shows sequence similarity to ascorbate oxidase is expressed in developing pollen. Molecular characterization and analysis of promoter activity in transgenic tobacco plants

A phospholipid uptake system in the model plant Arabidopsis thaliana

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