The promoter of the rice gene <i>GOS2</i> is active in various different monocot tissues and binds rice nuclear factor ASF‐1

Páter, Béla; Mark, F. van der; Rueb, Saskia; Katagiri, Fumiaki; Chua, Nam‐Hai; Schilperoort, R. A.; Hensgens, L. A. M.

doi:10.1046/j.1365-313x.1992.00999.x

Cited by 50 publications

(23 citation statements)

References 39 publications

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“…To enable the overexpression of the OsNAC10 genes in rice, their full-length cDNAs were isolated and linked to the RCc3 promoter (Xu et al, 1995) for rootspecific expression and to the GOS2 promoter (de Pater et al, 1992) for constitutive expression, thereby generating the constructs RCc3:OsNAC10 and GOS2: OsNAC10. These constructs were introduced into rice using Agrobacterium tumefaciens-mediated transformation (Hiei et al, 1994), which yielded 15 to 20 independent transgenic lines per construct.…”

Section: Identification Of Stress-inducible Rice Nac Domain Genesmentioning

confidence: 99%

“…Here, we report the role of OsNAC10, one of the effective members selected from the prescreening, in drought tolerance. Overexpression of OsNAC10 under the control of GOS2 (de Pater et al, 1992), a constitutive promoter, and RCc3 (Xu et al, 1995), a root-specific promoter, improved plant tolerance of transgenic rice to drought, high salinity, and low temperature during the vegetative stage of growth. In addition, RCc3:OsNAC10 plants showed significantly enhanced drought tolerance at the reproductive stage, with a grain yield increase of 25% to 42% over the controls under field drought conditions.…”

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Root-Specific Expression ofOsNAC10Improves Drought Tolerance and Grain Yield in Rice under Field Drought Conditions

et al. 2010

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Drought poses a serious threat to the sustainability of rice (Oryza sativa) yields in rain-fed agriculture. Here, we report the results of a functional genomics approach that identified a rice NAC (an acronym for NAM [No Apical Meristem], ATAF1-2, and CUC2 [Cup-Shaped Cotyledon]) domain gene, OsNAC10, which improved performance of transgenic rice plants under field drought conditions. Of the 140 OsNAC genes predicted in rice, 18 were identified to be induced by stress conditions. Phylogenic analysis of the 18 OsNAC genes revealed the presence of three subgroups with distinct signature motifs. A group of OsNAC genes were prescreened for enhanced stress tolerance when overexpressed in rice. OsNAC10, one of the effective members selected from prescreening, is expressed predominantly in roots and panicles and induced by drought, high salinity, and abscisic acid. Overexpression of OsNAC10 in rice under the control of the constitutive promoter GOS2 and the root-specific promoter RCc3 increased the plant tolerance to drought, high salinity, and low temperature at the vegetative stage. More importantly, the RCc3: OsNAC10 plants showed significantly enhanced drought tolerance at the reproductive stage, increasing grain yield by 25% to 42% and by 5% to 14% over controls in the field under drought and normal conditions, respectively. Grain yield of GOS2: OsNAC10 plants in the field, in contrast, remained similar to that of controls under both normal and drought conditions. These differences in performance under field drought conditions reflect the differences in expression of OsNAC10-dependent target genes in roots as well as in leaves of the two transgenic plants, as revealed by microarray analyses. Root diameter of the RCc3: OsNAC10 plants was thicker by 1.25-fold than that of the GOS2:OsNAC10 and nontransgenic plants due to the enlarged stele, cortex, and epidermis. Overall, our results demonstrated that root-specific overexpression of OsNAC10 enlarges roots, enhancing drought tolerance of transgenic plants, which increases grain yield significantly under field drought conditions. Plants respond and adapt to abiotic stresses to survive under adverse conditions. Upon exposure of plants to such stresses, many genes are induced, and their products are involved in the protection of cellular machinery from stress-induced damage (Bray, 1993;Thomashow, 1999;Shinozaki et al., 2003). The expression of stress-related genes is largely regulated by specific transcription factors. The overexpression of such transcription factor genes often results in activation of many functional genes related to the particular stress conditions, consequently conferring stress tolerance. For example, the DREB1A/CBF3 gene in transgenic Arabidopsis (Arabidopsis thaliana) activates expression of its stress-related downstream genes, thereby enhancing stress tolerance (Liu et al., 1998;Kasuga et al., 1999).The rice (Oryza sativa) and Arabidopsis genomes each encode more than 1,300 transcriptional regulators, which account for 6% of the estimated total nu...

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Section: Identification Of Stress-inducible Rice Nac Domain Genesmentioning

confidence: 99%

mentioning

confidence: 99%

Root-Specific Expression ofOsNAC10Improves Drought Tolerance and Grain Yield in Rice under Field Drought Conditions

et al. 2010

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“…The GOS2 (rice eukaryotic translation initiation factor1-like gene) promoter has been shown to drive expression throughout the whole plant body (de Pater et al, 1992), while the RCc3 (rice lipid transfer proteinlike gene) promoter does so in a root-specific manner (Xu et al, 1995). Fifty independent lines for each construct were generated, and to eliminate somaclonal variation, successive field selection of T1 to T4 plants (4 years) was performed to identify elite lines that grew normally, without stunting.…”

Section: Oserf71 Overexpression In Rice Roots Enhances Drought Resistmentioning

confidence: 99%

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

et al. 2016

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Plant responses to drought stress require the regulation of transcriptional networks via drought-responsive transcription factors, which mediate a range of morphological and physiological changes. AP2/ERF transcription factors are known to act as key regulators of drought resistance transcriptional networks; however, little is known about the associated molecular mechanisms that give rise to specific morphological and physiological adaptations. In this study, we functionally characterized the rice (Oryza sativa) drought-responsive AP2/ERF transcription factor OsERF71, which is expressed predominantly in the root meristem, pericycle, and endodermis. Overexpression of OsERF71, either throughout the entire plant or specifically in roots, resulted in a drought resistance phenotype at the vegetative growth stage, indicating that overexpression in roots was sufficient to confer drought resistance. The root-specific overexpression was more effective in conferring drought resistance at the reproductive stage, such that grain yield was increased by 23% to 42% over wild-type plants or whole-body overexpressing transgenic lines under drought conditions. OsERF71 overexpression in roots elevated the expression levels of genes related to cell wall loosening and lignin biosynthetic genes, which correlated with changes in root structure, the formation of enlarged aerenchyma, and high lignification levels. Furthermore, OsERF71 was found to directly bind to the promoter of OsCINNAMOYL-COENZYME A REDUCTASE1, a key gene in lignin biosynthesis. These results indicate that the OsERF71-mediated drought resistance pathway recruits factors involved in cell wall modification to enable root morphological adaptations, thereby providing a mechanism for enhancing drought resistance.

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“…First, its sequence was verified by sequencing a full-length cDNA clone that was isolated from a rice cell suspension cDNA library using a PCRamplified probe corresponding to a partial Orysa; KRP1 cDNA sequence. To investigate the possible role of Orysa;KRP1 in rice development, we generated transgenic plants overexpressing the gene under the control of the constitutive rice GOS2 promoter (de Pater et al, 1992). Quantitative evaluation of transcript levels following transformation showed that the Orysa;KRP1 gene was overexpressed in all four independent transformation events analyzed (Supplemental Fig.…”

Section: Generation Of Orysa;krp1-overexpressing Linesmentioning

confidence: 99%

“…The Orysa;KRP1 cDNA was linked at its 5# end to the GOS2 promoter (de Pater et al, 1992) and at its 3# end to the ZEIN terminator (Wu et al, 1993).…”

Section: Generation Of Rice Krp1 Oe Transgenic Lines and Growth Condimentioning

confidence: 99%

The Cyclin-Dependent Kinase Inhibitor Orysa;KRP1 Plays an Important Role in Seed Development of Rice

et al. 2006

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Kip-related proteins (KRPs) play a major role in the regulation of the plant cell cycle. We report the identification of five putative rice (Oryza sativa) proteins that share characteristic motifs with previously described plant KRPs. To investigate the function of KRPs in rice development, we generated transgenic plants overexpressing the Orysa;KRP1 gene. Phenotypic analysis revealed that overexpressed KRP1 reduced cell production during leaf development. The reduced cell production in the leaf meristem was partly compensated by an increased cell size, demonstrating the existence of a compensatory mechanism in monocot species by which growth rate is less reduced than cell production, through cell expansion. Furthermore, Orysa;KRP1 overexpression dramatically reduced seed filling. Sectioning through the overexpressed KRP1 seeds showed that KRP overproduction disturbed the production of endosperm cells. The decrease in the number of fully formed seeds was accompanied by a drop in the endoreduplication of endosperm cells, pointing toward a role of KRP1 in connecting endocycle with endosperm development. Also, spatial and temporal transcript detection in developing seeds suggests that Orysa;KRP1 plays an important role in the exit from the mitotic cell cycle during rice grain formation.

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The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF‐1

Cited by 50 publications

References 39 publications

Root-Specific Expression ofOsNAC10Improves Drought Tolerance and Grain Yield in Rice under Field Drought Conditions

Root-Specific Expression ofOsNAC10Improves Drought Tolerance and Grain Yield in Rice under Field Drought Conditions

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

The Cyclin-Dependent Kinase Inhibitor Orysa;KRP1 Plays an Important Role in Seed Development of Rice

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