The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1

Páter, Béla; Mark, F. van der; Rueb, Saskia; Katagiri, Fumiaki; Chua, Nam‐Hai; Schilperoort, R. A.; Hensgens, L. A. M.

doi:10.1111/j.1365-313x.1992.00837.x

Cited by 29 publications

(51 citation statements)

References 37 publications

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“…The yeast gene is called suil [ 61 ] and is a suppressor locus of translational initiator mutants. Furthermore, the putative rice protein shows 75~ similarity to the amino acid sequence encoded by a mouse cDNA clone for which homologous sequences have been found also in man and bovine species [45].…”

Section: Introductionmentioning

confidence: 87%

“…These genes are GOS2, GOS5 and GOS9. They have been described in detail and the complete nucleotide sequence of the cDNA and genomic sequences have been published [ 19,21,43,44,45]. In the present study we used the genes GOS2 and GOS5.…”

Section: Introductionmentioning

confidence: 99%

“…Expression is also observed in flags, panicles, internodes, nodes and roots from mature T309 and IR36 plants and in in vitro cell suspension cultures initiated from the variety japonica T309. The size of its m R N A is 800 nucleotides [45].…”

Section: Introductionmentioning

confidence: 99%

“…1A). The GOS2 m R N A contains an open reading frame of 345 nucleotides potentially encoding a protein of 115 amino acids [45]. The putative GOS2 protein is 52Yo identical to a yeast protein that functions during the recognition of initiation A U G codons [61] in the translation process.…”

Section: Introductionmentioning

confidence: 99%

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Transient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass

et al. 1993

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Transcriptional and translational fusions were made between the reading frame coding for beta-D-glucuronidase and sequences of either a constitutively expressed rice gene (GOS2) involved in initiation of translation or a light-inducible rice gene (GOS5). The transient expression of the fusions was studied via particle bombardment of seedling tissues of rice, perennial ryegrass and barley. Furthermore, the results of transient and stable expression were compared for cell suspensions of four rice varieties, one barley variety and one perennial ryegrass variety. The GOS2-gusA fusions were active in all three monocots studied. Best results were obtained for a construct having both a transcriptional and a translational fusion as well as intron and exon sequences (PORCEHyg). The level of GUS activity was in the range of activities as obtained by the 35S CaMV promoter transcriptionally fused to gusA. The gusA fusion with the light-inducible gene (GOS5) was active in green seedling tissues of all monocots studied. Also a weak expression compared to the GOS2 constructs was found in stably transformed rice callus. The gusA fusions with the mannopine synthase promoters 1' and 2' of the TR-DNA were transiently expressed at lower levels in cell suspensions than PORCEHyg. For stably transformed rice callus the expression of the GOS2-gusA fusion often decreased during prolonged subculture. This decrease in GUS activity and the various GUS-staining phenotypes of transgenic calli are explained by the presence of different cell types in the suspensions used and in the calli. It is presumed that the nature of the cells and their relative contribution in the calli change drastically upon further subculture.

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Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass

et al. 1993

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“…The sequence around the initiation codon is conserved between rice, yeast, and human: CGT ATG T. In spite of the absence of the essential A or G at -3, and the G at +1 [19], the rice homolog GOS2 is expressed at high levels [20]. Apparently, expression is mainly regulated at the transcriptional level.…”

Section: --mentioning

confidence: 99%

The amino acid sequence of eukaryotic translation initiation factor 1 and its similarity to yeast initiation factor SUI1

1995

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Eukaryotic initiation factor elF-1 was purified from rabbit reticnlocytes. Amino acid sequence analysis revealed that the protein contained a blocked amino-terminus. After cleavage with the endoproteinase Asp-N, three peptides were sequenced. The obtained partial sequences were identical to sequences of SUIIISO1, the human homologue of the yeast translation initiation factor SUI1. The SUI1 gene product was identified as a protein involved in the recognition of the protein synthesis initiation codon. A similar mode of action has been suggested for eIF-1.

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RicemicroRNA171f/SCL6module enhances drought tolerance by regulation of flavonoid biosynthesis genes

Choi

Park

et al. 2022

Plant Direct

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Plants have evolved sophisticated defense systems to enhance drought tolerance. These include the microRNA (miRNA) group of small noncoding RNAs that act as post‐transcriptional regulators; however, details of the mechanisms by which they confer drought tolerance are not well understood. Here, we show that osa‐MIR171f , a member of osa ‐ MIR171 gene family, is mainly expressed in response to drought stress and regulates the transcript levels of SCARECROW‐LIKE6‐I ( SCL6‐I ) and SCL6‐II in rice ( Oryza sativa ). The SCL6 genes are known to be involved in shoot branching and flag leaf morphology. Osa‐MIR171f‐ overexpressing ( osa ‐ MIR171f ‐OE) transgenic plants showed reduced drought symptoms compared with non‐transgenic (NT) control plants under both field drought and polyethylene glycol (PEG)‐mediated dehydration stress conditions. Transcriptome analysis of osa‐MIR171f ‐OE plants and osa‐mir171f ‐knockout (K/O) lines generated by clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) revealed that osa ‐mature‐miR171a‐f ( osa ‐miR171) regulates the expression of flavonoid biosynthesis genes, consequently leading to drought tolerance. This upregulation in the osa‐MIR171f‐ OE plants, which did not occur in NT control plants, was observed under both normal and drought conditions. Our findings indicate that osa ‐miR171 plays a role in drought tolerance by regulating SCL6‐I and SCL6‐II transcript levels.

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The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1

Cited by 29 publications

References 37 publications

Transient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass

Transient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass

The amino acid sequence of eukaryotic translation initiation factor 1 and its similarity to yeast initiation factor SUI1

RicemicroRNA171f/SCL6module enhances drought tolerance by regulation of flavonoid biosynthesis genes

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