The promiscuous potential of cellulase in degradation of polylactic acid and its jute composite

Karimi-Avargani, Mina; Bazooyar, Faranak; Biria, Davoud; Zamani, Akram; Skrifvars, Mikael

doi:10.1016/j.chemosphere.2021.130443

Cited by 12 publications

(9 citation statements)

References 57 publications

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“…Moisture content and morphological aspects of biofiller were defined, prior its thermal processes with PLA, to prevent the degradation of the matrix via hydrolysis. [ 2–4 ] The biofiller is composed of sub‐millimetric irregular particles ( Figure ) most of which are below 500 µm, that is, nozzle diameter.…”

Section: Resultsmentioning

confidence: 99%

“…Fused deposition modeling (FDM) is the most used 3D printing methodology for its cost‐effectiveness, affordability, simplicity, reliability, minimal waste, and wide material availability. Among them, poly(lactic) acid (PLA) is one of the most widely used materials thanks to its cost‐effectiveness, good mechanical properties, and biodegradability, [ 1–4 ] thus also reducing fossil‐based plastic pollution. Furthermore, PLA also obtained from renewable resources, it is produced commercially on a large scale at a reasonable price, though still not comparable with conventional thermoplastic commodities’ one (e.g., polyethylene, polypropylene, and polystyrene).…”

Section: Introductionmentioning

confidence: 99%

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A New Biocomposite Material Based on Wheat Waste and Suitable for 3D Printing Applications

Giani

Mazzocchetti

Benelli

et al. 2022

Macromolecular Symposia

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Biopolymers, such as poly(lactic) acid (PLA), which is obtained through green synthesis pathways from renewable resources, has attracted considerable interest in recent years because of the increasing need to reduce petroleum‐based plastic pollution and bringing their prices comparable with conventional thermoplastic commodities’ price (e.g., polyethylene, polypropylene, and polystyrene). The present work investigates the employment of 10% wt of natural materials, deriving from wheat milling process, as biofiller of PLA to develop a biocomposite filament suitable for 3D‐printing technique. The inclusion of a cost‐free natural material leads to a strong reduction of the whole material cost. Implementing this new class of composite material to additive manufacturing technique allows to dramatically reduce the environmental impact of 3D printed products.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Biocomposite Material Based on Wheat Waste and Suitable for 3D Printing Applications

Giani

Mazzocchetti

Benelli

et al. 2022

Macromolecular Symposia

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show abstract

“…14,19,20 Additionally, the regeneration of virgin L-lactate monomers for resynthesis via enzymatic recycling avoids downgrading of PLLA products that is associated with mechanical recycling practices. 14,21 Identification and characterization of novel PLLA depolymerizing enzymes may provide a diverse array of tools that will be useful in enzymatic recycling processes; however, the promiscuous nature of naturally occurring PLLA depolymerizing enzymes, whose primary functions are as proteases, 22,23 esterases, 23,24 cellulases, 25 and other hydrolases, 20 leads to low catalytic efficiencies against non-naturally occurring polymers like PLLA. 26 to plastic depolymerizing enzymes; 26 however, there are few studies demonstrating protein engineering efforts for PLLA depolymerizing enzymes.…”

Section: ■ Introductionmentioning

confidence: 99%

Synergistic Mutations Create Bacillus Subtilisin Variants with Enhanced Poly-l-Lactic Acid Depolymerization Activity

Cannon

Reynolds

2023

Biomacromolecules

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Enzymatic recycling of poly-l-lactic acid (PLLA) plastic has recently become an area of interest; however, investigation of enzymatic mechanisms and engineering strategies to improve activity remains limited. In this study, we have identified a subtilisin from Bacillus pumilus that has the ability to depolymerize high-molecular-weight PLLA. We performed a comparative, mutational analysis of this enzyme with a less active homologue from Bacillus subtilis to determine residues favored for activity. Our results demonstrate that both enzymes contain residues favored for PLLA depolymerization, with the generation of several hyperactive variants. In silico modeling suggests that increases in activity are due to opening of the binding pockets and increased surface hydrophobicity. Combinations of hyperactive mutations have synergistic effects with the generation of subtilisin variants with 830- and 184-fold increases in activity for B. subtilis and B. pumilus subtilisins, respectively. One B. pumilus subtilisin variant can visibly dissolve high-molecular-weight PLLA films.

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“…The phenomenon should be closely related to the free hydroxyl groups on the surface of HNTs and the entrained moisture in the cavity of HNTs, which accelerates the thermal decomposition of PLA. [ 41,42 ] In addition, the space between PLA and HNTs allows the accumulation of volatile pyrolysis products of PLA, thereby accelerating degradation, [ 43 ] which is further supported by the lack of close contact between HNTs and PLA in SEM. However, PMMA‐HNTs were uniformly dispersed in PLA and tightly bound to the matrix.…”

Section: Resultsmentioning

confidence: 99%

Preparation of intercalating halloysite by in situ synthesized polymethyl methacrylate and its effects on the crystallization behaviors and mechanical properties of polylactic acid composites

et al. 2022

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Polylactic acid/halloysites (PLA/HNTs) composites with different nanotube contents were prepared by solution blending. Using unmodified HNTs and polymethyl methacrylate intercalated halloysites (PMMA-HNTs), their structures and properties were comparatively investigated. It is found that the glass transition temperature (T g ) of the composites is lower than that of pure PLA.In addition, both HNTs and PMMA-HNTs promoted the crystallization of PLA. However, due to the good dispersion of PMMA-HNTs in the matrix, the crystallinity of PMMA-HNTs-modified PLA is higher than that of HNTsmodified PLA loaded with the same nanoparticles. The study on the mechanical properties of PMMA-HNTs and HNTs-modified PLA composites suggested that when the content of PMMA-HNTs was not higher than 4 wt%, it could simultaneously improve the elongation at break and tensile strength. Thermal stability studies showed that the T 5% and T max of the two composites did not improve significantly, but the amount of carbon residues increased. This study provides a new strategy for modifying HNTs and preparing PLA composites with good comprehensive properties.

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The promiscuous potential of cellulase in degradation of polylactic acid and its jute composite

Cited by 12 publications

References 57 publications

A New Biocomposite Material Based on Wheat Waste and Suitable for 3D Printing Applications

A New Biocomposite Material Based on Wheat Waste and Suitable for 3D Printing Applications

Synergistic Mutations Create Bacillus Subtilisin Variants with Enhanced Poly-l-Lactic Acid Depolymerization Activity

Preparation of intercalating halloysite by in situ synthesized polymethyl methacrylate and its effects on the crystallization behaviors and mechanical properties of polylactic acid composites

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