The Promiscuous Life of Plant NUCLEAR FACTOR Y Transcription Factors

Petroni, Katia; Kumimoto, Roderick W.; Gnesutta, Nerina; Calvenzani, V.; Fornari, Monica; Tonelli, Chiara; Holt, Ben F.; Mantovani, Roberto

doi:10.1105/tpc.112.105734

Cited by 309 publications

(333 citation statements)

References 114 publications

(200 reference statements)

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“…They bind DNA at CCAAT sites as heterotrimeric complexes composed of single subunits and are reported to be regulating drought responses in many plant species (Stephenson et al 2007;Hackenberg et al 2012). NFYA's expression is regulated at transcriptional and post transcriptional level by miR169 (Petroni et al 2012) and has positive and negative effects on gene expression (González et al 2012). Upregulation of NFYA in tolerant clones of Hevea in this study indicates its strong association with drought tolerance.…”

Section: Resultsmentioning

confidence: 55%

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Luke

Sathik

Thomas

et al. 2015

Physiol Mol Biol Plants

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In order to meet the ever rising global demand for natural rubber, cultivation of Hevea is being extended to nontraditional regions of India where extreme climatic conditions like drought and low temperature negatively influence the crop performance. In order to ensure maximum productivity, identification of drought tolerant clones of Hevea which can cope up with stress and give better crop yield is essential. Several attempts have been made previously to identify genes that are associated with drought tolerance in Hevea. In the present study, quantitative expression analysis was made using quantitative PCR for seven drought associated transcripts in four clones of Hevea with varying levels of drought tolerance. Among the seven genes studied, Mitogen Activated Protein (MAP) kinase, Myeloblastosis (Myb) transcription factor, C-repeat responsive element/Dehydration Responsive Element (CRT/DRE) binding factor and Nuclear Factor Y subunit A (NFYA) showed a positive association with drought tolerance. Transcripts of ascorbate peroxidase and heat shock protein 70 (HSP 70) did not show any correlation with drought tolerance. Interestingly, catalase gene was found down regulated in all the clones under drought condition. The possible role of these genes based on their level of gene expression in four different clones of Hevea with varying levels of drought tolerance is discussed.

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Section: Resultsmentioning

confidence: 55%

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Luke

Sathik

Thomas

et al. 2015

Physiol Mol Biol Plants

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“…Members of the heterotrimeric, nucleosome-like NF-Y family of TFs are targets of the NIN protein (Soyano et al, 2013) and mediate early infection and nodule development processes (Petroni et al, 2012;Laloum et al, 2013Laloum et al, , 2014. NF-YA subunits bind to conserved CCAAT motifs in promoters, where they recruit the histone 2A/2B-like NF-YC and NF-YB subunits (Nardini et al, 2013), which also bind DNA, albeit nonspecifically.…”

Section: Hierarchical Clustering Highlights Nod Factor-and Et-dependementioning

confidence: 99%

Deep Sequencing of the Medicago truncatula Root Transcriptome Reveals a Massive and Early Interaction between Nodulation Factor and Ethylene Signals

et al. 2015

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The legume-rhizobium symbiosis is initiated through the activation of the Nodulation (Nod) factor-signaling cascade, leading to a rapid reprogramming of host cell developmental pathways. In this work, we combine transcriptome sequencing with molecular genetics and network analysis to quantify and categorize the transcriptional changes occurring in roots of Medicago truncatula from minutes to days after inoculation with Sinorhizobium medicae. To identify the nature of the inductive and regulatory cues, we employed mutants with absent or decreased Nod factor sensitivities (i.e. Nodulation factor perception and Lysine motif domain-containing receptor-like kinase3, respectively) and an ethylene (ET)-insensitive, Nod factor-hypersensitive mutant (sickle). This unique data set encompasses nine time points, allowing observation of the symbiotic regulation of diverse biological processes with high temporal resolution. Among the many outputs of the study is the early Nod factorinduced, ET-regulated expression of ET signaling and biosynthesis genes. Coupled with the observation of massive transcriptional derepression in the ET-insensitive background, these results suggest that Nod factor signaling activates ET production to attenuate its own signal. Promoter:b-glucuronidase fusions report ET biosynthesis both in root hairs responding to rhizobium as well as in meristematic tissue during nodule organogenesis and growth, indicating that ET signaling functions at multiple developmental stages during symbiosis. In addition, we identified thousands of novel candidate genes undergoing Nod factor-dependent, ET-regulated expression. We leveraged the power of this large data set to model Nod factor-and ET-regulated signaling networks using MERLIN, a regulatory network inference algorithm. These analyses predict key nodes regulating the biological process impacted by Nod factor perception. We have made these results available to the research community through a searchable online resource.

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“…2, 3; Tables 1, 2). Our conclusion is supported by three OsNF-YC and three OsNF-YB proteins that are clustered in the same phylogenetic clade (Thirumurugan et al 2008;Petroni et al 2012;Laloum et al 2013) as AtNF-YC3/-YC4/-YC9 proteins and AtNF-YB2/-YB3 proteins, which are known to influence flowering (Kumimoto et al 2008, 19 2010). However, the possibility that the OsNF-YC subunits interact with other OsNF-YB subunits in other developmental processes, cannot be excluded.…”

Section: Osnf-yc Proteins Form Heterodimeric Complexes With Osnf-yb Pmentioning

confidence: 52%

“…6), and the report of Thirumurugan et al (2008) that OsNF-YB2, which is involved in chloroplast biogenesis (Miyoshi et al 2003), interacts with OsNF-YC subunits in yeast. OsNF-YC4-RNAi and OsNF-YC6-RNAi plants could be due to the functional redundancy of these genes with the homologous OsNF-YC genes (Thirumurugan et al 2008;Petroni et al 2012;Laloum et al 2013). Transgenic plants over-expressing the OsNF-YC genes showed either delayed or accelerated flowering only under LD conditions ( Fig.…”

Section: Osnf-yc Proteins Form Heterodimeric Complexes With Osnf-yb Pmentioning

confidence: 99%

“…Individual NF-Y subunits identified in plant species are also known to play an essential role in the regulation of developmental processes (Meinke et al 1994;Li et al 2008;Yamamoto et al 2009;Petroni et al 2012;Laloum et al 2013;Mu et al 2013). NF-YB and NF-YC subunits are found as CONSTANS (CO)-interacting proteins in tomato and Arabidopsis thaliana (BenNaim et al 2006;Wenkel et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

Kim

Park

Jang

et al. 2015

Planta

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Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11).Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.

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The Promiscuous Life of Plant NUCLEAR FACTOR Y Transcription Factors

Cited by 309 publications

References 114 publications

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Deep Sequencing of the Medicago truncatula Root Transcriptome Reveals a Massive and Early Interaction between Nodulation Factor and Ethylene Signals

OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

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