The proliferative lifespan of adult sheep fibroblasts can be conditionally extended by tetracycline-inducible expression of human telomerase reverse transcriptase
Abstract:Forced expression of human telomerase reverse transcriptase (hTERT) has been used to immortalize mammalian cells. Here, we report conditional extension of proliferative lifespan of adult sheep somatic cells by introducing tetracycline-inducible (Tet-on) expression of hTERT. After transfecting adult sheep fibroblasts with the vector for Tet-on induced conditional expression of hTERT, we obtained several hTERT-positive clones that exhibited extended-lifespan under the induction of tetracycline analogue, doxycycl… Show more
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