The proliferation associated nuclear element (PANE1) is conserved between mammals and fish and preferentially expressed in activated lymphoid cells

The PANE1 gene encodes a novel human minor histocompatibility antigen that is selectively expressed in B-lymphoid cells and B-CLL

“…PANE1 transcript c was originally identified as a gene highly expressed in ␤-catenin-transformed mouse mammary tissues, 34 but a subsequent report using semiquantitative RT-PCR and Northern blot analysis in human samples indicated preferential expression of this transcript in activated lymphoid cells and did not detect expression in cell lines of mammary origin. 26 These results are consistent with our observations using quantitative RT-PCR, although we additionally observed significant expression of transcript c in testis but did not assess expression in mammary cells. PANE1 transcript c is evolutionarily conserved between mammals and zebrafish, and no functional motifs have been identified in the protein that it encodes.…”

“…The finding that PANE1 transcript k was selectively expressed in resting CD19 ϩ cells and CD19 ϩ B-CLL appeared to contradict a previous report 26 that PANE1 is preferentially expressed in activated lymphoid cells. In view of the unique exon present in transcript k that is absent from the 10 other reported PANE1 transcripts, we hypothesized that transcript k and other PANE1 transcripts would show distinct expression profiles in resting compared with activated cells.…”

“…PANE1 transcript c is evolutionarily conserved between mammals and zebrafish, and no functional motifs have been identified in the protein that it encodes. 26 Ectopic expression of murine 34 or human 26 PANE1 transcript c indicated that the protein encoded by this transcript is preferentially present in the nucleus in nonconfluent cells, but is excluded from the nucleus during cell division or upon attainment of confluence. Finally, a mass-spectrometric analysis of the centromere complex from HeLa interphase cells indicated that PANE1 colocalizes with various centromere proteins, although the identity of the PANE1 transcript variant encoding this protein was not indicated.…”

The pseudo GTPase CENP-M drives human kinetochore assembly

“…CENP-M was initially identified for its high expression in proliferating cells, and named accordingly PANE1, for proliferation associated nuclear element 1 (Bierie et al, 2004). Subsequently, CENP-M was shown to be closely associated with CENP-A, as well as with CENP-H, CENP-K, CENP-I, CENP-L, CENP-N, and CENP-T (Obuse et al, 2004; Foltz et al, 2006; Izuta et al, 2006; Okada et al, 2006).…”

The ABCs of CENPs

“…CENP-M was originally isolated as proliferation associated nuclear element 1 (PANE1), which is highly expressed in proliferating cells such as activated lymphoid cells and tumors (Bierie et al 2004;Renou et al 2003). Later, CENP-M was identified as ICEN39 as well as a NAC and CENP-H/I-associated component (Foltz et al 2006;Izuta et al 2006;Okada et al 2009).…”