The progress of leaf rust research in wheat

Prasad, Pramod; Savadi, Siddanna; Bhardwaj, S. C.; Gupta, P. K.

doi:10.1016/j.funbio.2020.02.013

Cited by 63 publications

(31 citation statements)

References 213 publications

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“…Among them, the genes Lr21 , Lr22a , Lr32 , Lr39 / Lr41, Lr40 , Lr42 , and Lr43 have been transferred from Ae. tauschii into wheat 63 , of which Lr21 (GenBank: FJ876280.1) and Lr22a (KY064064.1) have been cloned and sequenced 64 , 65 . Among these two sequenced LR genes, we could identify a putative orthologue gene for Lr22a i.e.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of two contrasting resistant and susceptible Aegilops tauschii accessions to wheat leaf rust (Puccinia triticina) using RNA-sequencing

Dorostkar

Dadkhodaie

Ebrahimie

et al. 2022

Sci Rep

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Leaf rust, caused by Puccinia triticina Eriks., is the most common rust disease of wheat (Triticum aestivum L.) worldwide. Owing to the rapid evolution of virulent pathotypes, new and effective leaf rust resistance sources must be found. Aegilops tauschii, an excellent source of resistance genes to a wide range of diseases and pests, may provide novel routes for resistance to this disease. In this study, we aimed to elucidate the transcriptome of leaf rust resistance in two contrasting resistant and susceptible Ae. tauschii accessions using RNA-sequencing. Gene ontology, analysis of pathway enrichment and transcription factors provided an apprehensible review of differentially expressed genes and highlighted biological mechanisms behind the Aegilops–P. triticina interaction. The results showed the resistant accession could uniquely recognize pathogen invasion and respond precisely via reducing galactosyltransferase and overexpressing chromatin remodeling, signaling pathways, cellular homeostasis regulation, alkaloid biosynthesis pathway and alpha-linolenic acid metabolism. However, the suppression of photosynthetic pathway and external stimulus responses were observed upon rust infection in the susceptible genotype. In particular, this first report of comparative transcriptome analysis offers an insight into the strength and weakness of Aegilops against leaf rust and exhibits a pipeline for future wheat breeding programs.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of two contrasting resistant and susceptible Aegilops tauschii accessions to wheat leaf rust (Puccinia triticina) using RNA-sequencing

Dorostkar

Dadkhodaie

Ebrahimie

et al. 2022

Sci Rep

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“…In addition to Lr65 , four wheat leaf rust resistance genes was located on the short arm of chromosome 2A, including Lr17 , Lr37 , and Lr45 ( McIntosh et al, 2008 ; Prasad et al, 2020 ). Lr11 was previously located on chromosome 2AS ( Soliman et al, 1964 ), but recent studies have shown that Lr11 is located distal to chromosome 2DS ( Darino et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Fine Mapping of the Leaf Rust Resistance Gene Lr65 in Spelt Wheat ‘Altgold’

et al. 2021

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Wheat leaf rust (also known as brown rust), caused by the fungal pathogen Puccinia triticina Erikss. (Pt), is one by far the most troublesome wheat disease worldwide. The exploitation of resistance genes has long been considered as the most effective and sustainable method to control leaf rust in wheat production. Previously the leaf rust resistance gene Lr65 has been mapped to the distal end of chromosome arm 2AS linked to molecular marker Xbarc212. In this study, Lr65 was delimited to a 0.8 cM interval between flanking markers Alt-64 and AltID-11, by employing two larger segregating populations obtained from crosses of the resistant parent Altgold Rotkorn (ARK) with the susceptible parents Xuezao and Chinese Spring (CS), respectively. 24 individuals from 622 F2 plants of crosses between ARK and CS were obtained that showed the recombination between Lr65 gene and the flanking markers Alt-64 and AltID-11. With the aid of the CS reference genome sequence (IWGSC RefSeq v1.0), one SSR marker was developed between the interval matched to the Lr65-flanking marker and a high-resolution genetic linkage map was constructed. The Lr65 was finally located to a region corresponding to 60.11 Kb of the CS reference genome. The high-resolution genetic linkage map founded a solid foundation for the map-based cloning of Lr65 and the co-segregating marker will facilitate the marker-assisted selection (MAS) of the target gene.

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“…Puccinia triticina populations throughout the world are genetically diverse in terms of their molecular architecture as well as the combinations and complexity of their virulence patterns. This is influenced by the co-evolution of the pathogen with diverse wheats and genetic recombination of Pt races emerging from various Triticum species (Kolmer and Liu, 2000;Liu et al, 2014;Prasad et al, 2020). The genetic characteristics of all Pt populations is typical of diploid/dikaryotic populations, with a life cycle summarized by Bolton et al (2008).…”

Section: Genetic Variability and Distribution Of Pt In Southern Africamentioning

confidence: 99%

Breeding Wheat for Durable Leaf Rust Resistance in Southern Africa: Variability, Distribution, Current Control Strategies, Challenges and Future Prospects

et al. 2020

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Leaf or brown rust of wheat caused by Puccinia triticina (Pt) is one of the most damaging diseases globally. Considerable progress has been made to control leaf rust through crop protection chemicals and host plant resistance breeding in southern Africa. However, frequent changes in the pathogen population still present a major challenge to achieve durable resistance. Disease surveillance and monitoring of the pathogen have revealed the occurrence of similar races across the region, justifying the need for concerted efforts by countries in southern Africa to develop and deploy more efficient and sustainable strategies to manage the disease. Understanding the genetic variability and composition of Pt is a pre-requisite for cultivar release with appropriate resistance gene combinations for sustainable disease management. This review highlights the variability and distribution of the Pt population, and the current control strategies, challenges and future prospects of breeding wheat varieties with durable leaf rust resistance in southern Africa. The importance of regular, collaborative and efficient surveillance of the pathogen and germplasm development across southern Africa is discussed, coupled with the potential of using modern breeding technologies to produce wheat cultivars with durable resistance.

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The progress of leaf rust research in wheat

Cited by 63 publications

References 213 publications

Comparative transcriptome analysis of two contrasting resistant and susceptible Aegilops tauschii accessions to wheat leaf rust (Puccinia triticina) using RNA-sequencing

Comparative transcriptome analysis of two contrasting resistant and susceptible Aegilops tauschii accessions to wheat leaf rust (Puccinia triticina) using RNA-sequencing

Fine Mapping of the Leaf Rust Resistance Gene Lr65 in Spelt Wheat ‘Altgold’

Breeding Wheat for Durable Leaf Rust Resistance in Southern Africa: Variability, Distribution, Current Control Strategies, Challenges and Future Prospects

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