The prognostic value and potential drug target of phosphatase and tensin homolog in breast cancer patients

Xu, Feng; Zhang, Chao; Cui, Jianxiu; Li, Jun; Li, Jie; Jiang, Hongchuan

doi:10.1097/md.0000000000008000

Cited by 12 publications

(8 citation statements)

References 37 publications

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“…PTEN is a well-characterized tumor suppressor gene, which antagonizes the phosphoinositol-3-kinase/protein kinase B (PKB or Akt) signaling pathway [ 12 ]. The PTEN gene acts as a tumor suppressor gene in multiple cancers, including breast cancer [ 13 , 14 ], nasopharyngeal carcinoma [ 15 ], renal cell carcinoma [ 16 ], non-small-cell lung cancer [ 17 ], hepatocellular carcinoma [ 18 ], human NK/T-cell lymphoma [ 19 ], and osteosarcoma [ 20 ]. The loss of function of PTEN in tumor cells results in the accumulation of critical cell messengers, which increases Akt phosphorylation and activity and leads to decreased apoptosis and/or increased mitogenic signaling [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1

et al. 2018

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BackgroundShikonin is a component of Chinese herbal medicine. The aim of this study was to investigate the effects of shikonin on cell migration of papillary thyroid cancer cells of the TPC-1 cell line in vitro and expression levels of the phosphate and tensin homolog deleted on chromosome 10 (PTEN) and DNA methyltransferase 1 (DNMT1) genes.Material/MethodsThe Cell Counting Kit-8 (CCK-8) assay was performed to evaluate the proliferation of TPC-1 papillary thyroid cancer cells, and the normal thyroid cells, HTori-3, in vitro. A transwell motility assay was used to analyze the migration of TPC-1 cells. Western blot was performed to determine the expression levels of PTEN and DNMT1 genes. A methylation-specific polymerase chain reaction (PCR) (MSP) assay was used to evaluate the methylation of PTEN.ResultsFollowing treatment with shikonin, the cell survival rate of TPC-1 cells decreased in a dose-dependent manner; the inhibitory effects on HTori-3 cells were less marked. Shikonin inhibited TPC-1 cell migration and invasion in a dose-dependent manner. The methylation of PTEN was suppressed by shikonin, which also reduced the expression of DNMT1 in a dose-dependent manner, and increased the expression of PTEN. Overexpression of DNMT1 promoted the migration of TPC-1 cells and the methylation of PTEN. Levels of protein expression of PTEN in TPC-1 cells treated with shikonin decreased, and were increased by DNMT1 knockdown.ConclusionsShikonin suppressed the expression of DNMT1, reduced PTEN gene methylation, and increased PTEN protein expression, leading to the inhibition of TPC-1 cell migration.

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Section: Introductionmentioning

confidence: 99%

Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1

et al. 2018

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“…Breast cancer (BC) is one the most common cancers among women. [ 1 , 2 ] Its incidence and mortality contribute to a major public health problem. [ 3 – 5 ] It has been estimated that 25.1% new cases of BC and 14.7% deaths from this cancer occurred in 2012.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound combined with fine needle aspiration cytology for the assessment of axillary lymph nodes in patients with early stage breast cancer

Zhang

Meng

et al. 2018

Medicine

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This study aimed to explore the clinical usefulness of ultrasound-guided fine needle aspiration cytology (USG-FNAC) for the evaluation of axillary lymph nodes in patients with early stage breast cancer (BC) among the Chinese Han female population.Around 124 patients with early stage BC were included in this retrospective study. All patients underwent USG-FNAC (group A). Patients with proven metastasis also underwent axillary lymph node dissection (ALND) (group B). In addition, sentinel lymph node biopsy (SLNB) was performed 2 to 5 hours prior to the surgery.The sensitivity, specificity, accuracy, and positive predictive value (PPV) of axillary ultrasound were 75.0%, 75.0%, 75.0%, and 82.6%, respectively, while for USG-FNAC, they were 80.8%, 100.0%, 88.7%, and 100.0%, respectively. Significant differences were found in specificity, accuracy, and PPV between the 2 procedures (P < .05).The results of this study demonstrated that USG-FNAC was effective for selecting patients with early stage BC using ALND or SLNB among the Chinese Han female population.

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“…A number of studies have investigated Burkitt's lymphoma; however, the specific pathogenesis of the disease is not fully understood. Our previous studies found that inhibition of the PI3K pathway can inhibit the proliferation of Burkitt's lymphoma, and abnormal expression of phosphatase and tensin homolog (PTEN) is common in solid tumors, including breast cancer, kidney cancer, esophageal cancer, endometrial cancer (6)(7)(8) and diffuse large B cell lymphoma (12). Therefore, a relationship between PTEN overexpression and the incidence of Burkitt's lymphoma has been indicated.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, PTEN has been indicated to negatively regulate the PI3K/AKT signaling pathway. As a negative regulatory factor, PTEN exhibits mutations, deletions, methylation and abnormal activity in solid tumors (6)(7)(8). PTEN is also abnormally expressed in B-cell lymphomas, including diffuse large B-cell lymphoma (9), resulting in the aberrant activation of the PI3K/AKT pathway.…”

Section: Introductionmentioning

confidence: 99%

Role and mechanism of PTEN in Burkitt's lymphoma

Xin

et al. 2020

Oncol Rep

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The aim of the present study was to explore the possible mechanisms of phosphatase and tensin homolog (PTEN) in the pathogenesis of Burkitt's lymphoma, and provide novel information that can be used in the targeted treatment of this disease. PTEN lentiviral overexpression vector and short-hairpin PTEN silencing vectors were constructed. The effect of PTEN on the growth and proliferation of CA46 and RAJI cells was analyzed using a Cell Counting Kit-8 assay. Apoptosis was detected by Hoechst 33342 and propidium iodide double staining. Flow cytometry was used to analyze the cell cycle. A Transwell chamber was used to detect cell migration and invasion abilities. Western blot analysis was used to detect related protein changes. The mechanism of the effect of PTEN on the biological characteristics of Burkitt's lymphoma cells was subsequently analyzed. The results revealed that PTEN inhibited the proliferation of CA46 and RAJI cells by downregulating the expression of p-AKT, It was indicated that the upregulation of proapoptotic proteins (including Bad and Bax) induced apoptosis, regulated cyclin (including P53, P21, CDK4, CDK6, cyclin D3 and cyclin H) to inhibit cell cycle progression, and mediated epithelial-mesenchymal transition-like cell markers (including E-cadherin, N-cadherin, β-catenin, TCF-8, vimentin, Slug and Snail) to inhibit cell migration and invasion. In conclusion, the tumor-suppressor gene PTEN inhibited the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) signaling pathway and inhibited the proliferation and migration of Burkitt's lymphoma cells, induced apoptosis and cell cycle arrest, thus playing a crucial role in the pathogenesis of Burkitt's lymphoma.

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The prognostic value and potential drug target of phosphatase and tensin homolog in breast cancer patients

Abstract: Our meta-analysis demonstrates that PTEN loss is of particular importance for predicting breast cancer aggressiveness and poor prognosis. PTEN is a potential drug target for the development of individualized treatment in BC patients.

Cited by 12 publications

References 37 publications

Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1

Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1

Ultrasound combined with fine needle aspiration cytology for the assessment of axillary lymph nodes in patients with early stage breast cancer

Role and mechanism of PTEN in Burkitt's lymphoma

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