The prognostic significance of T cell receptor β gene rearrangements and idiotype-reactive T cells in multiple myeloma

Abstract

Clonal T cell populations with idiotype specificity are present to CD3 stimulation which boosts antitumour effector mechin the peripheral blood of a proportion of patients with multiple anisms. 15 It has also been shown that increased numbers of myeloma. We have identified the presence of both T cell subactivated suppressor T cells or NK cells are a good prognostic populations with a specificity for autologous immunoglobin factor at diagnosis. 16,17 fragments and T cell receptor ␤ gene rearrangements in periph-The presence of T cell receptor ␤ gene rearrangements has vations provide evidence for the existence of idiotype-reactive

Mono/oligoclonal T and NK cells are common in chronic myeloid leukemia patients at diagnosis and expand during dasatinib therapy

“…Similarly, in patients with multiple myeloma, the presence of clonal T cells was related to superior prognosis. 52 In accord, long-lasting LGL expansions with clonal TCR ␥/␦ gene rearrangements have been described in a subset of patients receiving an allogeneic stem cell transplant, which were associated with better overall survival, suggesting a graft-versus-leukemia activity. 53,54 We, therefore, speculate that dasatinib treatment reverts the tolerance mechanism and restores the function of unresponsive, exhausted leukemiaspecific cytotoxic cells.…”

Clonal cytotoxic T cells are expanded in myeloma and reside in the CD8+CD57+CD28− compartment

“…On the basis of Southern blot analysis of the TCR␤, which detects monoclonal expansions representing at least 4% to 5% of the total Ficoll-separated peripheral blood cells, 8 our laboratory previously reported on the prognostic significance of true T-cell clonal populations in MM. 9 We found that MM patients exhibiting expanded T-cell clones had a better prognosis than those without detectable clonal bands on Southern blot analysis. 9 Flow cytometric analysis offers the means to isolate and characterize such clones in terms of specificity and function.…”

“…9 We found that MM patients exhibiting expanded T-cell clones had a better prognosis than those without detectable clonal bands on Southern blot analysis. 9 Flow cytometric analysis offers the means to isolate and characterize such clones in terms of specificity and function. Previous flow analysis indicated that the expanded T-cell clones, as judged by specific positivity with anti-TCRV ␤ antibodies, expressed the phenotype of cytotoxic T cells and were predominantly CD8 ϩ , CD57 ϩ , CD28 Ϫ , and perforin positive.…”

αβ T-cell receptor bias in disease and therapy (Review)

“…TRBV overexpres- sion is more frequently observed in CD4 + T cells than in CD8 + T cells from patients with B-CLL (23), suggesting that leukemic B cells present MHC class II-restricted peptides to CD4 + T cells. Specific T cells from some patients with myeloma can recognize tumor-derived idiotypic immunoglobulin, and patients with these idiotype-reactive T cells are likely to have a better prognosis (26). No change in TRBV usage was detected between T cells that were cultured in a culture medium alone and with phytohemagglutinin stimulation, suggesting that the tumor-associated antigen-mediated selection of monoclonal and oligoclonal TCR families from stimulated T cells were indeed tumor specific (27).…”