The prognostic significance of bacterial DNA in patients with decompensated cirrhosis and suspected infection

Abstract

BactDNA in sterile body fluids did not indicate increased mortality in cirrhotic patients with suspected infection. Using multiplex PCR for risk stratification cannot be recommended in these patients.

Functional polymorphisms of innate immunity receptors are not risk factors for the non‐SBP type bacterial infections in cirrhosis

“…Likewise in patients with decompensated cirrhosis an increased translocation of bacterial DNA fragments into ascitic fluid was found in the presence of the NOD2 risk variant p.G908R . Moreover, there was increased transition of pathological BT to culture‐positive SBP in the case of the same NOD2 variant . Furthermore, TLR2 (−16934 T>A, rs4696480) and TLR4 (D299G, rs4986790) polymorphisms were associated with an increased systemic antigen burden as well, described by the serum level of lipoteichoic acid, LPS, and bacterial‐DNA …”

“…32 There is evidence that variants of the NOD2 gene, 33,34 and various TLR 35 there was increased transition of pathological BT to culture-positive SBP in the case of the same NOD2 variant. 37,38 Furthermore, TLR2 (−16934 T>A, rs4696480) and TLR4 (D299G, rs4986790) polymorphisms were associated with an increased systemic antigen burden as well, described by the serum level of lipoteichoic acid, LPS, and bacterial-DNA. 35 In our study we applied both serological and clinical approaches to assess the impact of PRR genetic variants to BT.…”

Low serum transferrin correlates with acute‐on‐chronic organ failure and indicates short‐term mortality in decompensated cirrhosis

“…At inclusion, 9 mL of venous blood were obtained, centrifuged, aliquoted and serum was stored at −80°C. A subset of 161 samples was reported in a previously published study . The presented cohort study conformed to the ethical guidelines of the 1975 Declaration of Helsinki and the collection of samples and the prospective follow‐up were approved by the Internal Review Board (Ethikkommission des Universitätsklinikums Jena [Ethics committee of the Jena University Hospital]; 2880‐08/10, 3683‐02/3).…”

“…A subset of 161 samples was reported in a previously published study. 19 The presented cohort study conformed to the ethical guidelines of the Demographic and clinical data were collected at baseline. Chronic viral hepatitis, autoimmune and metabolic liver diseases were assessed according to standard patient care.…”

Prognostic value of bacterial infection in acute and chronic liver failure

“…Indeed, the lack of correlation between survival and circulating bacterial DNA observed in critically ill cirrhotic patients with suspected infection or in cirrhotic patients with ascites in the studies by Bruns et al . and Appenrodt et al ., respectively, further questions the clinical significance of this marker in cirrhosis . The different results observed among studies could be due to heterogeneity in the populations tested and to limitations of the molecular techniques used to detect bacterial DNA, including a lack of standardized methods and false‐positive results due to contamination.…”