The Prognostic Role of Glutathione Peroxidase 1 and Immune Infiltrates in Glioma Investigated Using Public Datasets

Lv, Shan; Luo, Haitao; Huang, Kai; Zhu, Xingen

doi:10.12659/msm.926440

Cited by 6 publications

(4 citation statements)

References 43 publications

(40 reference statements)

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“…Increasing evidence has demonstrated that Gpx1 exhibited a critical role in the progress of brain diseases. For instance, Gpx1 was found to be increased in glioma [ 18 ]. Sharma et al found that Gpx1 silencing promoted the proinflammatory response and activated vascular endothelium [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Knockdown of PVT1 Exerts Neuroprotective Effects against Ischemic Stroke Injury through Regulation of miR-214/Gpx1 Axis

Liu

Wang

Jing

et al. 2022

BioMed Research International

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Previous studies have reported that lncRNA PVT1 was closely related to ischemic stroke. Here, the role of PVT1 in ischemic stroke and the underlying mechanism were investigated. OGDR-stimulated PC12 cells were used to construct a cell model to mimic ischemic stroke. si-PVT1, miR-214 mimic, inhibitor, or the negative controls were transfected into PC12 cells prior to OGDR treatment. PVT1, miR-214, and Gpx1 expression was measured by qRT-PCR and western blotting assays. Cell proliferation and apoptosis were tested by CCK-8 assay and western blotting. The expression levels of inflammatory factors were determined by ELISA Kit. Results showed that PVT1 was increased significantly in OGDR PC12 cells. PVT1 knockdown significantly enhanced cell viability and attenuated cell apoptosis, ROS generation, and inflammation in OGDR PC12 cells. More importantly, PVT1 or Gpx1 was a target of miR-214. Mechanistically, PVT1 acted as a competing endogenous RNA of miR-214 to regulate the downstream gene Gpx1. In conclusion, PVT1 knockdown attenuated OGDR PC12 cell injury by modulating miR-214/Gpx1 axis. These findings offer a potential novel strategy for ischemic stroke therapy.

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Section: Introductionmentioning

confidence: 99%

Knockdown of PVT1 Exerts Neuroprotective Effects against Ischemic Stroke Injury through Regulation of miR-214/Gpx1 Axis

Liu

Wang

Jing

et al. 2022

BioMed Research International

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“… 16 A bioinformatics study showed that expression level of GPX1 is related to the prognosis of patients with glioma. 44 In LGG, we found that SEPHS2 expression was negatively correlated with five selenoprotein genes ( GPX3, SELENOK, SELENOO, TXNRD2 , and TXNRD3 ) expression, which suggested that SEPHS2 might have antagonistic effects with these genes. However, SEPHS2 expression was positively correlated with nine genes ( DIO2, GPX2, SELENOF, SELENOH, SELENOI, SELENOM, SELENON, SELENOT , and TXNRD1 ) expression, which indicated that SEPHS2 have potential synergistic effects with these genes.…”

Section: Discussionmentioning

confidence: 84%

Bioinformatics Analyses Reveal the Prognostic Value and Biological Roles of SEPHS2 in Various Cancers

Zhang¹,

Zhao²,

Mao³

et al. 2021

IJGM

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Purpose Selenophosphate synthetase 2 (SEPHS2) has been shown to regulate selenoprotein biosynthesis by catalyzing the synthesis of active selenium donor selenophosphate. SEPHS2 influences the survival of tumor cells. However, few studies have explored the expression level and prognostic of SEPHS2 in various cancers. Methods The expression of SEPHS2 in human tumor tissues and normal adjacent tissues was analyzed in The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Human Protein Atlas (HPA), and UALCAN databases. Cox regression analysis and Kaplan–Meier curve analysis were performed to analyze the association of SEPHS2 expression with the prognosis of cancer patients. The expression and prognosis of SEPHS2 in gliomas were further verified using the Chinese Glioma Genome Atlas (CGGA) dataset. The relationship between SEPHS2 and immune infiltration, tumor mutational burden (TMB), microsatellite instability (MSI), and neoantigens was comprehensively explored using a TCGA cohort. The mechanism by which SEPHS2 regulates tumor progression was explored by using the STRING database. A nomogram was constructed using the R software to predict the overall survival (OS) of patients with brain lower grade glioma (LGG). Results SEPHS2 was highly expressed in many cancers including LGG. Its high expression was significantly associated with poor OS, disease-free survival (DFS), and progression-free survival (PFS). Univariate and multivariate Cox analyses showed that SEPHS2 was an independent prognostic factor for LGG. Concordance index and calibration curves revealed that the nomogram had good predictive performance (concordance index: 0.791; 95% CI: 0.732–1). A significant correlation was found between SEPHS2 and immune infiltration, TMB, MSI, and tumor neoantigens across diverse cancers. Enrichment analysis showed that SEPHS2 may regulate the PPAR signaling pathway. Conclusion SEPHS2 expression regulates tumor development and it is a potential treatment target and prognostic biomarker, especially for lower grade glioma.

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“…In a study on cell cultures of gliomas with both the wild type and the IDH1 mutation, indirect evidence was found that glutathione peroxidase levels are altered in cells with the IDH1 mutation [ 40 ]. In addition, it was demonstrated that the glutathione peroxidase 1 expression dramatically increased with the IDH-wildtype [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Relationship between Glutathione-Dependent Enzymes and the Immunohistochemical Profile of Glial Neoplasms

et al. 2022

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This research aimed to investigate the relationships between the parameters of glutathione metabolism and the immunohistochemical characteristics of glial tumors. Postoperative material from 20 patients with gliomas of different grades of anaplasia was analyzed. Bioinformatic analysis of the interactions between the gliomas’ immunohistochemical markers and their glutathione-dependent enzymes was carried out using the STRING, BioGrid, while Signor databases revealed interactions between such glioma markers as IDH and p53 and the glutathione exchange enzymes (glutathione peroxidase, glutathione reductase, glutathione S-transferase). The most pronounced relationship with glutathione metabolism was demonstrated by the level of the nuclear protein Ki67 as a marker of proliferative activity, and the presence of the IDH1 mutation as one of the key genetic events of gliomagenesis. The glutathione system is an active participant in the body’s antioxidant defense, involving the p53 markers and MGMT promoter methylation. It allows characterization of the gliomal cells’ status at different stages of tumor development.

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The Prognostic Role of Glutathione Peroxidase 1 and Immune Infiltrates in Glioma Investigated Using Public Datasets

Cited by 6 publications

References 43 publications

Knockdown of PVT1 Exerts Neuroprotective Effects against Ischemic Stroke Injury through Regulation of miR-214/Gpx1 Axis

Knockdown of PVT1 Exerts Neuroprotective Effects against Ischemic Stroke Injury through Regulation of miR-214/Gpx1 Axis

Bioinformatics Analyses Reveal the Prognostic Value and Biological Roles of SEPHS2 in Various Cancers

Relationship between Glutathione-Dependent Enzymes and the Immunohistochemical Profile of Glial Neoplasms

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