The Profiles of Lactobacilllus Microflora and Local Immune Response of the Vaginal Mucosa in the Diagnosis of Vaginal Infections

Budilovskaya, Olga V.; Krysanova, Anna A.; Shipitsyna, Elena; Perever, zeva; Vorobyova, Nadezhda E.; Gerasimova, Ekaterina N.; Grigoryev, Aleksey N.; Savi, cheva

doi:10.29296/24999490-2020-03-07

Cited by 4 publications

(2 citation statements)

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“…Currently, the “Lactobacilli Typing” test for the identification of the total lactobacilli count in addition to the most common vaginal lactobacilli species ( L. crispatus , L. iners , L. jensenii , L. gasseri , L. johnsonii , L. vaginalis , and L. acidophilus ) has been introduced into practice [ 37 ].…”

Section: The Application Of Molecular Testing In the Differential Dia...mentioning

confidence: 99%

“…For instance, increased expression of IL-18, GATA3, and CD68 mRNAs was demonstrated in samples with L. crispatus dominance. The combination of microbiological and immunological markers represents a favorable prognostic tool in terms of vaginal microbiota stability of the physiological microbiocenosis of the vagina [ 37 ].…”

Section: Local Immune Response Evaluationmentioning

confidence: 99%

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Molecular Testing for the Diagnosis of Bacterial Vaginosis

Savicheva

2023

IJMS

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Previously established diagnostic approaches for the diagnosis of bacterial vaginosis (BV), such as the Amsel criteria or the Nugent scoring system, do not always correspond to modern trends in understanding the etiology and pathogenesis of polymicrobial conditions. Inter-examiner variability and interpretation of data complicate the wet mount microscopy method. Gram staining of smears does not always provide reliable information regarding bacterial taxa, biofilms, or vaginal dysbiosis. Therefore, the introduction of molecular techniques into clinical practice is extremely relevant. Molecular approaches allow not only the diagnosis of BV but also provide an assessment of microbial composition, which is especially important in the differential diagnosis of vaginal infections. The current review represents an expert opinion on BV diagnosis and is based on extensive experience in the field of vaginal infection diagnosis and treatment.

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Section: The Application Of Molecular Testing In the Differential Dia...mentioning

confidence: 99%

Section: Local Immune Response Evaluationmentioning

confidence: 99%

Molecular Testing for the Diagnosis of Bacterial Vaginosis

Savicheva

2023

IJMS

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Immune-mediated mechanisms of the inflammatory response in women with combined infections of the lower genital tract

Dikke

Sukhanov

Kukarskaya

et al. 2021

Akušerstvo, ginekologiâ i reprodukciâ

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Aim: to increase the effectiveness of combination therapy and reduce frequency of recurrences of inflammatory diseases in the lower genital tract of combined etiology.Materials and Мethods. There was conducted a prospective, randomized, blind, comparative clinical study. 80 women with vaginal microbiota disorders were examined randomized into 2 groups: group I (n = 40) – antibiotic therapy was carried out in combination with the preparation Superlimph, group II (n = 40) – a standalone antibacterial therapy; group III consisted of 20 patients lacking gynecological diseases considered to provide with control laboratory parameters. Clinical and laboratory methods were used: microscopy of vaginal smears, detection of viruses and sexually transmitted infections, measurement of serum cytokine levels – interleukins (IL-1â, IL-2, IL-4, IL-6, IL-8, IL-10), tumor necrosis factor alpha, (TNF-á), interferon gamma (IFN-ã).Results. It was found that clinical recovery occurred in 90.0 and 70.0 % of patients in group I and group II (р = 0.02), respectively. Microbiological recovery was observed in 100.0 and 67.5 % of patients, respectively (р = 0.003). In group I, the isolation of herpes simplex virus type 2 was found in 80.0 and 45.2 % of patients before and after treatment (p < 0.001), respectively, whereas in group II – in 82.5 and 77.5 %, respectively (p = 0.58), between groups – p < 0.001. Before treatment the level of all cytokines, excepting IFN-ã, was higher than the reference values in both groups: in group I, their magnitude decreased after treatment (p < 0.001) and corresponded to the reference values; in group II no changes occurred. Three months after treatment, no relapses were observed in group I, whereas frequency of relapses in group II was 22.5 %, within 1 year – 0 and 35.1 %, respectively. Predictors of recurrent dysbiosis (IL-2, IL-6 and TNF-á in increased concentrations after a course of treatment) were identified.Conclusion. Treatment with a combined broad-spectrum antimicrobial drug along with topical immunomodulatory agent containing exogenous cytokines is an effective strategy for treating concomitant dysbiosis and preventing relapse.

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Azoximer bromide-modulated immune response in aerobic vaginitis

Dikke¹,

Ostromensky²,

Kucheryavaya³

2022

Akušerstvo, ginekologiâ i reprodukciâ

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Aim: to evaluate the efficacy of azoximer bromide along with a combined antimicrobial drug in treatment of patients with aerobic vaginitis (AV).Materials and Methods. There was conducted a prospective, randomized, parallel group study with enrolled 60 patients diagnosed with AV and 10 apparently healthy women (control group III). Group I (n = 30) received azoximer bromide (10 vaginal insertion according to the scheme, course of 10 days) and a combined antimicrobial drug, while Group II (n = 30) received only antimicrobial drug (course of 10 days). Microscopy of vaginal discharge smears, polymerase chain reaction method was used, detection of cytokines – interleukins (IL) and tumor necrosis factor-alpha (TNF-α) in vaginal washings was performed by ELISA. The study of neutrophil extracellular traps (NETs) was performed by ELISA, and DNA-containing strands ejected by neutrophils were determined by fluorescence method.Results. Recovery from disease was found in 100 % (30/30) and 86.7 % (26/30) in group I and group II, respectively. After treatment, serum level of IL-8 was revealed to decline in group I from 35.2 to 5.5 pg/ml (p = 0.05), in group II – from 33.4 to 5.3 pg/ml (p = 0.04), also not differ (p > 0.05) from the control values (5.2 pg/ml). The level of IL-1β also decreased after treatment in group I from 51.5 to 15.1 pg/ml (p = 0.002), in group II – from 57.9 to 20.1 pg/ml (p = 0.03), which also did not differ (p > 0.05) from the control values (16.7 pg/ml). The IL-10 level in both main groups decreased slightly (from 0.26 and 0.24 to 0.16 pg/ml in both groups), which was higher by 1.8-fold than in the control group (0.09 pg/ml; p < 0.001). The number of ejected NETs in both main groups decreased significantly after treatment, reaching control level. No side effects were observed, compliance and acceptability were 100 % in both groups. The frequency of AV relapses within 3 months was observed in 3.3 % (1/30) and 14.3 % (4/28) patients, after 6 months – in 14.3 % (4/28) and in 20.0 % (5/25) patients in groups I and II, respectively. Using the logit regression model, demonstrated that AV relapse might occur with a 50 %-probability at TNF-α level < 0.1 pg/ml after treatment (sensitivity – 67 %, specificity – 79 %). The risk of relapses in group I, in contrast to group II, showed a downward trend.Conclusions. The use of azoximer bromide and a combined broad-spectrum antimicrobial drug is effective in AV treatment, exerts a modulating effect on immune response parameters contributes to lowering relapse rate.

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The Profiles of Lactobacilllus Microflora and Local Immune Response of the Vaginal Mucosa in the Diagnosis of Vaginal Infections

Cited by 4 publications

References 0 publications

Molecular Testing for the Diagnosis of Bacterial Vaginosis

Molecular Testing for the Diagnosis of Bacterial Vaginosis

Immune-mediated mechanisms of the inflammatory response in women with combined infections of the lower genital tract

Azoximer bromide-modulated immune response in aerobic vaginitis

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