The production of α-amylase (E.C.3.2.1.1.) byBacillus amyloliquefaciens, in a complex and a totally defined synthetic culture medium

Hewitt, Christopher J.; Solomons, G. L.

doi:10.1007/bf01570050

Cited by 35 publications

(22 citation statements)

References 7 publications

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“…5). It has been previously found that organic nitrogen sources like peptone and yeast extract usually have stimulating effects [33] and our findings are similar to them. Various other organic nitrogen sources have also been reported to support maximum α-amylase production by various Bacillus species [34]- [39].…”

Section: Bacillus Subtilissupporting

confidence: 91%

Isolation and Characterization of Bacillus Subtilis KC3 for Amylolytic Activity

VIJAYALAKSHMI¹,

Sushma²,

Abha³

et al. 2012

IJBBB

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Abstract-Microbial α-amylase is a highly demanded industrial enzyme with extensive commercial applications in various sectors. Studies were carried out with a bacterial strain producing extracellular α-amylase, isolated from rhizospheric soil of Euphorbia hirta. The isolate was gram positive, motile rod, bearing terminal endospore. It exhibited >98% similarity with the reference strains in the GenBank. The phylogenetic tree constructed on the basis of 16S rRNA gene sequences revealed that it clustered with the closest members of Bacillus subtilis and identified as Bacillus subtilis KC3. Furthermore, the effects of incubation period, temperature, pH, different carbon and nitrogen sources, metal salts and different substrate concentrations of the medium were optimized. The maximum enzyme production was found after 48 h (22.92 U/ml) of incubation at temperature 40°C and pH 7. The optimal temperature and pH for enzyme activity were 50°C and 6.5 respectively. Barley starch (27.27 U/ml) was observed to be the best inducer followed by corn starch (24.30 U/ml) and maltose (19.10 U/ml). The addition of glucose to the culture medium greatly reduced the synthesis of α-amylase (5.45 U/ml) which demonstrates that a classical glucose effect is operative in this organism. The effects of different metal ions (Ca 2+, Fe 2+ , Mg 2+ , Zn 2+ and Cu 2+ ) on amylolytic activity were investigated and it was found that 0.1% of Ca 2+ increased enzyme production (28.83U/ml), whereas other metal ions exhibited inhibitory effects. The enzyme production was maximum at 2% substrate (starch) concentration, which declined beyond it. These characteristics of Bacillus subtilis KC3, suggest that this is a promising isolate which merits further investigations for potential applications in various biotechnological processes.Index Terms-α-amylase, bacillus subtilis KC3, euphorbia hirta, rhizospheric soil

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Section: Bacillus Subtilissupporting

confidence: 91%

Isolation and Characterization of Bacillus Subtilis KC3 for Amylolytic Activity

VIJAYALAKSHMI¹,

Sushma²,

Abha³

et al. 2012

IJBBB

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“…AS-1 has been reported to be maximum in the presence of 0.02% CaCl 2 [21]. Our finding is in agreement with the previous studies on Bacillus amyloliquefaciens and Bacillus subtilis cultures with respect to α-amylase activity [32,33]. These observations may also be supported by the fact that amylase is a calcium metalloenzyme and increment in the Ca 2+ concentration up to 0.02% increases the bioavailability of Ca 2+ to the saturation leading to enhancement in enzyme production.…”

Section: Progress Curve Of α-Amylasesupporting

confidence: 93%

Production, Partial Purification and Characterization of α-Amylase from High Molecular Weight Polycyclic Aromatic Hydrocarbons (HMW-PAHs) Degrading Bacillus subtilis BMT4i (MTCC 9447)

Lily¹,

Bahuguna²,

Bhatt³

et al. 2012

Turk J Bioch

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“…8). Generally the organic nitrogen sources such as peptone and yeast extract are frequently enhanced the amylase production in the medium (Hewitt and Solomons, 1996).…”

Section: Fig 7: Effect Of Carbon Sources On Enzyme Productionmentioning

confidence: 99%

Isolation, screening and optimization of amylase production by a marine bacterium Bacillus subtilis SJ33

Sundarapandiyan¹,

Jayalakshmi²

2017

Int. J. Adv. Res. Biol. Sci.

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Enzymes are among the most important products obtained for human needs mainly through microbial sources. The aim of the present study was isolation, screening and optimization of the enzyme production by using the marine bacterium Bacillus subtilis SJ33. In the present study, the growth parameters showed the profound influence on the amylase production on the candidate species such as the maximum production of the enzyme obtained at 48 hrs of incubation and the optimum was pH 7. The temperature also influenced on the maximum production of the amylase, the optimum was 35C for higher production of the enzyme. The optimum salinity of 2.0% showed to maximum production of the amylase. In the present study, the starch and peptone was found to the best carbon and nitrogen sources to maximum production of the amylase. With optimized parameters in the mass scale medium maximum growth of 2.89 OD and enzyme activity of 64U/ml/min was obtained at 48 hrs. of incubation.

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The production of α-amylase (E.C.3.2.1.1.) byBacillus amyloliquefaciens, in a complex and a totally defined synthetic culture medium

Cited by 35 publications

References 7 publications

Isolation and Characterization of Bacillus Subtilis KC3 for Amylolytic Activity

Isolation and Characterization of Bacillus Subtilis KC3 for Amylolytic Activity

Production, Partial Purification and Characterization of α-Amylase from High Molecular Weight Polycyclic Aromatic Hydrocarbons (HMW-PAHs) Degrading Bacillus subtilis BMT4i (MTCC 9447)

Isolation, screening and optimization of amylase production by a marine bacterium Bacillus subtilis SJ33

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