The Production of Tobacco

Garner, W. W.

doi:10.1097/00010694-194705000-00016

Cited by 15 publications

(18 citation statements)

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“…This is also a major tropical cash crop of considerable economic significance to Bangladesh. The plant tobacco belongs to the genus Nicotiana under the large family Solanaceae, the nightshade family (Garner, 1951). There are only two cultivated species under this genus viz., Nicotiana tabacum and Nicotiana rustica which was established by Carolas Linnaeus in 1753.…”

Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> regeneration of popular tobacco varieties of Bangladesh from leaf disc

Rahman

Alam

Hossain

et al. 1970

Bangladesh J. Agric. Res

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Regeneration ability of five Nicotiana varieties viz., Virginia, Jati, Motihari, CC Bengal and Sumatra were investigated via callus induction using leaf discs. Explants were cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators. Callus formation frequency was 67.20%. Among the varieties used, Motihari induced the highest percentage (97.50%) of callus followed by Jati (92.50%) in 2.0 rng/L Kinetin and 2.0 mg/L IAA. Shoots were induced from calli cultured on the same medium. Maximum shoot formation from leaf discs was 82.50% on medium supplemented with 2.0 mg/L Kinetin and 2.0 mg/L IAA. It was also revealed from this study that Motihari was the best variety for callus formation and subsequent plantlet regeneration which is a pre-requisite for vector mediated transformation for varietal improvement of Nicotiana species. The rooting response of regenerated shoots was observed by using 1 / 2 MS medium with IBA (0.0, 0.5, and 1.0 mg/L). The highest root formation was found in Motihari (90%) with ½ MS medium supplemented with 0.5 mg/L IBA. After that regenerated plantlets with plenty of roots were transferred successfully to pots and subsequently to the field.

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Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> regeneration of popular tobacco varieties of Bangladesh from leaf disc

Rahman

Alam

Hossain

et al. 1970

Bangladesh J. Agric. Res

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“…There is good evidence that this may be so. Hackett & Rawson (1974) have shown that when plants are not topped and suckered the leaves develop at a similar rate, but there are several reports that topping causes an overall delay in the maturation of tobacco crops (Avery 1934;Garner 1951;Woltz & Mason 1966). Some of these reports indicate that maturation of lower leaves, which are well developed at topping, is not affected by the treatment, but they do not indicate whether there is a progressive delay in maturation as the results presented ere would suggest.…”

Section: Discussionmentioning

confidence: 99%

Effects of leaf maturation on development of theSclerotinia/Botrytiscomplex of tobacco

Hartill

Campbell

1977

New Zealand Journal of Agricultural Research

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In field experiments natural infection of tobacco leaves by Botrytis cinerea Pers. ex Fr. and Sclerotinia sclerotiorum de Bary did not usually occur until approximately 2 weeks after the leaves had completed their main phase of expansion. Exceptions were infections originating from senescing corollas that had lodged on the leaves, but these early infections represented only a small proportion of the total infections that originated from fallen corollas. The age of " leaf at the time of infection was related to its position 011 the plant, a relationship affected by the "topping" and "suckering" treatment applied. When all the flowers were allowed to develop, the upper leaves became infected when they were slightly younger than the middle leaves, but when all the flower buds were removed at an early stage of development, leaf age at the time of infection increased with ascending leaf position. Infection spread faster in the lamina of old leaves than in that of young leaves. Sclerotinia destroyed lamina faster than did Botrytis. The rate of spread of infection within the lamina was not affected by removal of flowering heads.415

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“…Bailey (1958) found that in clover the starch content of leaves collected over a 28-day growth period showed no significant variation. Several estimates of starch content of tobacco leaves have been made (Garner 1951;Askew et al 1954) on samples collected at commercial ripeness or at unidentified age (Spoehr and Milner 1939) and these have indicated that starch percentage of dry weight is about 30.…”

Section: Introductionmentioning

confidence: 99%

Starch Changes in Developing and Senescing Tobacco Leaves

Matheson¹,

Wheatley²

1962

Aust. Jnl. Of Bio. Sci.

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SummaryThe quantitative changes in starch content during the growth and senescence of tobacco leaves have been followed. The starch content was low while the leaves were expanding but rapidly increased after expansion stopped. The maximum was reached when more than half of the original chlorophyll content had gone. The starch content was then reduced rapidly and when the leaf was all yellow there was only a small quantity of starch and this remained when the leaf turned brown.The viscosities, iodine affinities, fl-amylolysis limits, and granule sizes of isolated starch granules and the chain lengths of amylopectins at different ages of leaves have been determined. The viscosities and iodine affinities increased with increasing age of the leaf and the fl-amylolysis limits of the whole starch and chain length of the amylopectins were constant throughout the growth of the leaf. The average granule size increased as the leaf matured until maximum starch content was reached and decreased as the starch content decreased. It is suggested that in tobacco leaves another starch accumulation pattern of longer duration than 'diurnal accumulation exists. The degradation of the starch resembles the breakdown of starch in sprouting potato tubers and not that in germinating barley where amylases are the active enzymes.The residue after ethanol extraction of leaves at different stages of growth was fractionated into water-soluble material, a perchloric acid extract precipitated by iodine, and a perchloric acid extract not precipitated by iodine but precipitated by ethanol. Glucan was present in significant concentration only in the perchloric acid extract precipitated by iodine and this had the properties of starch. Paper chromatography indicated that non-starchy extracts were composed of galactose, arabinose, xylose, rhamnose, and uronic acid.

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The Production of Tobacco

Cited by 15 publications

References 0 publications

<i>In vitro</i> regeneration of popular tobacco varieties of Bangladesh from leaf disc

<i>In vitro</i> regeneration of popular tobacco varieties of Bangladesh from leaf disc

Effects of leaf maturation on development of theSclerotinia/Botrytiscomplex of tobacco

Starch Changes in Developing and Senescing Tobacco Leaves

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