The production of pig preimplantation embryos in vitro: Current progress and future prospects

Fowler, Katie E.; Mandawala, A.A.; Griffin, Darren K.; Walling, Grant A.; Harvey, Simon C.

doi:10.1016/j.repbio.2018.07.001

Cited by 57 publications

(38 citation statements)

References 118 publications

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“…We transferred 50 embryos into each recipient. Some reports suggest that the transfer of 16-20 embryos may be optimal to achieve normal pregnancy rates, but Fowler et al (8) mention using over 30 embryos to increase the likelihood of pregnancy. In our experiment, the transfer of embryos obtained from fertilized oocytes cultured with thymosin resulted in pregnancies in both recipients and the subsequent birth of 16 live piglets.…”

Section: Resultsmentioning

confidence: 99%

Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report

Poniedzialek-Kempny

Gajda

Rajska

et al. 2020

Medycyna Weterynaryjna

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The aim of the study was to examine the in vivo viability of in vitro-produced (IVP) porcine embryos obtained from oocytes matured with thymosin. The research material for this study consisted of immature pig oocytes obtained from ovaries after slaughter and ejaculated semen obtained from one boar. The immature oocytes were cultured in vitro until the metaphase II stage in a medium supplemented with thymosin (TMS). The presumptive zygotes obtained were cultured in vitro for 4-40 hours. The presumptive zygotes and 2-4-cell embryos were evaluated in vivo after transferring them to synchronized recipients. After the transfer of embryos from the experimental group into 2 recipients (50 embryos into each gilt) and the transfer of 50 embryos from the control group into 1 recipient, both gilts that had received embryos obtained by in vitro fertilization of oocytes matured with TMS became pregnant and delivered a total of 16 live piglets. After the transfer of embryos from the control group, no pregnancy was achieved. In conclusion, the results of our preliminary study suggest that the maturation of pig oocytes with thymosin supports the in vivo survival of in vitro produced embryos. It is important to note, that this was the first birth of piglets obtained after transfer of IVP embryos in Poland.

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Section: Resultsmentioning

confidence: 99%

Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report

Poniedzialek-Kempny

Gajda

Rajska

et al. 2020

Medycyna Weterynaryjna

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“…The research and development of different ART protocols in the porcine species, with the nal objective of improving their e ciency in a holistic sense, represents an area of increasing interest for two main reasons: rst, for the porcine species, the potential existence of an embryo market, allows farmers the exchange of genetic material without risk of disease transmission and reducing transportation costs (reviewed by [19]); second, for the human species, there is compelling evidence that the pig is an useful model to decipher the long term impact of each aspect of the ART without confounding factors such as those related to the fertility of the parents, a fact that has been recently reinforced with the discovery of the similarities between the pig and the human regarding the DNA methylation reprogramming events during the rst week of development [20].…”

Section: Discussionmentioning

confidence: 99%

Impact of Embryo Transfer on the phenotype of the offspring in pigs

París-Oller¹,

Matás²,

Romar³

et al. 2020

Preprint

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BackgroundThe increased use of assisted reproductive technologies led to a higher risk of specific alterations in the offspring, as already described in human and other mammalian species. Nonetheless, it is yet to be totally understood how and which specific techniques are inducing these changes. The use of animal models may represent an advantage to find out answers to these questions due to the minimization of confounding factors. The pig is not only a great human reproductive model, but it is also of interest in the livestock industry to develop further technologies that may enhance meat production. This study was designed to decipher the impact of one of these technologies, namely embryo transfer, on mother´s reproductive performance and on the phenotype of the offspring in pigs.ResultsEmbryo transfer of in vivo derived embryos (ET) showed similar results in terms of pregnancy length, litter size and total litter weight per sow than sows artificially inseminated (AI). Bodyweight at birth was higher for ET-females than AI-females, but this difference was no longer observed at day 15 of postnatal life. Some of the major findings after haematological analyses were that concentration of red blood cells, haemoglobin and haematocrit in female-ET piglets at day 3 showed higher values than AI-females. Additionally, higher concentration of white blood cells was found at day 3 in both ET-derived piglets (males and females) in comparison to AI-piglets. Biochemical analysis showed a higher level of albumin at day 3 for ET-derived male piglets than AI but ET-females showed lower levels of bilirubin than AI in the same day. Despite the statistical differences between groups, all values were within the normal ranges, so that we cannot give them any clinical significance.ConclusionsPiglets derived from in vivo embryo transfer seem to be phenotypically similar, which provides evidence that the embryo transfer procedure is a safe technique, without relevant health implications to the offspring. Additionally, these findings may be of interest to the pig industry regarding the reference values provided for haematological and biochemical parameters in this species.

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“…The basal media used for porcine IVF are the modified Tris‐buffered medium (mTBM), TCM‐199, Tyrode's albumin–lactate–pyruvate (TALP), and the porcine gamete medium (PGM) (Romar, Funahashi, & Coy, ). In regard to embryo culture, the most common media used are based on porcine zygote medium (PZM) and NCSU‐23; however, embryos cultured with other media, such as Beltsville embryo culture medium (BECM) and Whitten's medium (WM) have been able to develop to the blastocyst stage (Fowler, Mandawala, Griffin, Walling, & Harvey, ; Motta et al, ). Several reports have demonstrated that culture with PZM‐3 resulted in increased IVF and SCNT‐derived blastocyst development compared to NCSU‐23 (Im et al, ; Nánássy, Lee, Jávor, & Macháty, ; Wang et al, ; Yoshioka, Tanaka, Anas, Suzuki, & Iwamura, ).…”

Section: Transcriptomic and Metabolomic Approaches To Understand Preimentioning

confidence: 99%

“…The basal media used for porcine IVF are the modified Trisbuffered medium (mTBM), TCM-199, Tyrode's albumin-lactate-pyruvate (TALP), and the porcine gamete medium (PGM) (Romar, Funahashi, & Coy, 2016). In regard to embryo culture, the most common media used are based on porcine zygote medium (PZM) and NCSU-23; however, embryos cultured with other media, such as Beltsville embryo culture medium (BECM) and Whitten's medium (WM) have been able to develop to the blastocyst stage (Fowler, Mandawala, Griffin, Walling, & Harvey, 2018;Motta et al, 2018).…”

Section: Transcriptomic and Metabolomic Approaches To Understand Prmentioning

confidence: 99%

Applications of omics and nanotechnology to improve pig embryo production in vitro

Lucas

Chen

Seixas

et al. 2019

Molecular Reproduction Devel

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An appropriate environment to optimize porcine preimplantation embryo production in vitro is required as genetically modified pigs have become indispensable for biomedical research and agriculture. To provide suitable culture conditions, omics technologies have been applied to elucidate which metabolic substrates and pathways are involved during early developmental processes. Metabolomic profiling and transcriptional analysis comparing in vivo‐ and in vitro‐derived embryos have demonstrated the important role of amino acids during preimplantation development. Transcriptional profiling studies have been helpful in assessing epigenetic reprogramming agents to allow for the correction of gene expression during the cloning process. Along with this, nanotechnology, which is a highly promising field, has allowed for the use of engineered nanoplatforms in reproductive biology. A growing number of studies have explored the use of nanoengineered materials for sorting, labeling, and targeting purposes; which demonstrates their potential to become one of the solutions for precise delivery of molecules into gametes and embryos. Considering the contributions of omics and the recent progress in nanoscience, in this review, we focused on their emerging applications for current in vitro pig embryo production systems to optimize the generation of genetically modified animals.

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The production of pig preimplantation embryos in vitro: Current progress and future prospects

Cited by 57 publications

References 118 publications

Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report

Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report

Impact of Embryo Transfer on the phenotype of the offspring in pigs

Applications of omics and nanotechnology to improve pig embryo production in vitro

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