The production of mucosal ulceration in the rabbit gut by intra‐arterial injection of microspheres

The separation of DNA by gel electrophoresis provides a rapid method for determining size distributions of DNA in solution. Densitometric scanning of photographs of gels has been the standard method of analysis of agarose gels. However, analysis of photographs is complicated by the non-linear response of photographic film. Charged-coupled device video cameras have become popular for quantitative densitometry and we have used a charge-coupled device camera to image agarose gels to quantitate DNA damage. We compare video and photographic densitometry for quantitation of ultraviolet radiation (UV)-induced DNA damage and find that the two methods give equivalent results.

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Electrophoretic separation of nucleic acids: Evaluation by video and photographic densitometry

Freeman

Larcom

Thompson

1990

Electrophoresis

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Revisiting the rodent repairadox

Hanawalt

2001

Environ and Mol Mutagen

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Cultured rodent and human cells typically display similar clonal survival characteristics following exposure to ultraviolet light (UV). However, compared to human cells, cultured cells from mice, rats, and hamsters are generally deficient in excision repair of the most prominent DNA lesion produced by UV, the cyclobutane pyrimidine dimer. In light of recent studies on the control of nucleotide excision repair, we are beginning to understand the basis for this so-called "repairadox." The resolution of this issue is important because rodents are so widely employed as surrogates for humans in genetic toxicology. This article will review the evolution in our understanding of rodent DNA repair and will also "revisit" my early association with my graduate mentor and esteemed colleague, Dick Setlow, in his honor upon the attainment of his 80th birthday.

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Improved method for measuring the ensemble average of strand breaks in genomic DNA

Bespalov

Conconi

Zhang

et al. 2001

Environ and Mol Mutagen

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The cis-syn cyclobutane pyrimidine dimer (CPD) is the major photoproduct induced in DNA by low wavelength ultraviolet radiation. An improved method was developed to detect CPD formation and removal in genomic DNA that avoids the problems encountered with the standard method of endonuclease detection of these photoproducts. Since CPD-specific endonucleases make single-strand cuts at CPD sites, quantification of the frequency of CPDs in DNA is usually done by denaturing gel electrophoresis. The standard method of ethidium bromide staining and gel photography requires more than 10 microg of DNA per gel lane, and correction of the photographic signal for the nonlinear film response. To simplify this procedure, a standard Southern blot protocol, coupled with phosphorimage analysis, was developed. This method uses random hybridization probes to detect genomic sequences with minimal sequence bias. Because of the vast linearity range of phosphorimage detection, scans of the signal profiles for the heterogeneous population of DNA fragments can be integrated directly to determine the number-average size of the population.

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The production of mucosal ulceration in the rabbit gut by intra‐arterial injection of microspheres

Cited by 64 publications

References 6 publications

Electrophoretic separation of nucleic acids: Evaluation by video and photographic densitometry

Electrophoretic separation of nucleic acids: Evaluation by video and photographic densitometry

Revisiting the rodent repairadox

Improved method for measuring the ensemble average of strand breaks in genomic DNA

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