The production of anti‐hexapeptide antibodies which recognize the S7, L6 and L13 ribosomal proteins of Escherichia coli

Abstract

Here we report the synthesis of the N-terminal hexapeptide H-Pro-Arg-Arg-Arg-Val-Ile-OH of the E. coli ribosomal protein S7. the C-terminal hexapeptide H-Lys-Glu-Ala-Lys-Lys-Lys-OH of L6 and the C-terminal hexapeptide H-Pro-Gln-Val-Leu-Asp-Ile-OH of L13. All peptides were prepared by SPPS following the Fmoc-strategy, using DIC/HOBt and/or HBTU as coupling reagents and 2-chlorotrityl chloride resin as the solid support. The carrier linked synthetic peptides were injected into rabbits and elicited an anti-peptide response. These anti-hexapeptide antibodies were found to recognize the corresponding peptides and proteins.

Transcriptome analysis of dormant tomonts of the marine fish ectoparasitic ciliate Cryptocaryon irritans under low temperature

“…In the group B/C pairwise comparison, eight of the 40 up-regulated genes were not significantly changed in group B compared with Group A (Additional file 4 : Table S4 B/C column J), indicating a role in the regulation of tomonts entering a dormant state, including Ion (cation) channel family protein (ICFP) , Succinate dehydrogenase iron-sulfur subunit (SDIS) , Triosephosphate isomerase (TI) , Malonyl coa-acyl carrier protein transacylase (MCCPT) , Ribosomal protein L12 (RPL12); 50S ribosomal protein L13 (RPL13) , XP_001443344.1 and XP_672154.1 . SDIS is a primary dehydrogenase and donates electrons to the aerobic and energy-generating respiratory chain in eukaryotic mitochondria and numerous prokaryotes [ 49 ]; TI is a crucial enzyme in the glycolytic pathway, which catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (GAP) by an isomerization reaction without any cofactor or metal ion [ 50 ]; MCCPT is one of the essential enzymes in the FAS II system [ 51 ]; RPL12 is required for the ribosome to function with Elongation factor Tu, Initiation factor 2 , and Reversing factor [ 52 ]; RPL13 is essential for the formation of the RI* 50 intermediate particle [ 53 ]. However, the dynamic changes and functions of these genes during low-temperature dormancy and recovery of tomonts remain to be further studied.…”

Competitive binding effects on surface-enhanced Raman scattering of peptide molecules

“…Peptides of Y-P-P-P-W and W-P-P-P-Y were purchased from Elim Biopharmaceuticals. All other peptides were synthesized by an Fmoc solid phase peptide synthesis procedure using a 2-chlorotrityl resin loaded with the C-terminal amino acid residue [21]. Amino acid residues were added sequentially using coupling reagents of N-Hydroxybenzotriazole • H 2 O (HOBt) and 2-(lH-Benzotriazole-l-yl)-l, 1,3,3-tetramethylaminium hexafluorophosphate (HBTU).…”