The Production and Identification of Polyploids in Red Clover, White Clover and Lucerne

Evans, Alice M.

doi:10.1111/j.1469-8137.1955.tb06169.x

Cited by 46 publications

(33 citation statements)

References 5 publications

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“…There was also a difference in lOOO-seed weight (P < 0.01) between the diploid (2.10 g) and the tetraploid (3.15 g). Such seed size differences have been reported previomly by Evans (1967) and Anderson (1971).…”

supporting

confidence: 79%

Breeding a late-flowering tetraploid red clover for New Zealand

Anderson¹

1973

New Zealand Journal of Agricultural Research

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supporting

confidence: 79%

Breeding a late-flowering tetraploid red clover for New Zealand

Anderson¹

1973

New Zealand Journal of Agricultural Research

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“…The decrease of stomatal density correlated with the increase of ploidy levels observed in four-and five-week-old in vitro samples (Figure 2). Stomatal density was generally used to infer ploidy, whereby the increase in ploidy level often caused the number of stomata to decrease and guard cell size to increase (Evans 1954;Aryavand et al 2003;Chen et al 2009). All plants within the genus Agapanthus have the same number of chromosomes, therefore implying the importance of studies on nuclear DNA content to elucidate any differences between taxa (Ohri 1998).…”

Section: Resultsmentioning

confidence: 99%

Genetic stability ofin vitropropagated African blue lily (Agapanthus praecoxssp.minimus)

Yaacob

Taha

2014

Caryologia

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The present study presents cytological investigations of meristematic root cells of African lily (Agapanthus praecox ssp. minimus) grown in vivo and in vitro. Cellular parameters including mitotic index (MI), chromosome count and ploidy level (nuclear DNA content) were determined from 12 mm root tip segments of this species. The MI value was found to increase when cells were transferred from in vivo to in vitro conditions, perhaps due to early adaptations of cells to in vitro environment. However, addition of plant hormones in the culture medium was found to increase the MI values recorded for root cells grown in vitro. The mean chromosome number was generally stable (2n = 2x = 30) throughout the five-week culture period, indicating no occurrence of early somaclonal variation. Ploidy level analysis revealed that A. praecox root cells contained a high percentage of polyploid cells when grown in vitro after prolonged culture period. This showed that Agapanthus praecox ssp. minimus was not genetically stable when cultured in vitro, especially when subjected to prolonged culture period.

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“…The variation in pollen grain size in diploid and tetraploid clover plants is such that a clear distinction is not possible in all cases (EVANS 1955) . The measurement of stomata length is rather time consuming and only indicates the ploidy of the vegetative tissue .…”

Section: Discussionmentioning

confidence: 99%