The production and comparative evaluation of native and recombinant antigens for the fast serodiagnosis of cystic echinococcosis with dot immunogold filtration assay

Chen, X.; Lü, Xiaomei; Xia, Feng; Wen, Hao

doi:10.1111/pim.12151

Cited by 17 publications

(12 citation statements)

References 11 publications

(28 reference statements)

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“…Similarly, Tamer and colleagues [ 14 ], evaluating the performances of the VIRapid test, reported a better Se (96.8%) and same Sp (96% if sera from patients with other parasitoses were excluded – 87.5% if included) compared to our results, but they did not provide data on cyst characteristics; all CE patients included in their cohort where surgically confirmed, suggesting that predominantly active CE stages were included. Finally, Chen and colleagues [ 11 ], using sera from hospital cases, reported that the use of recombinant antigens in the DIGFA test might improve the specificity of the test, but at the expense of sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Diagnostic Accuracy of Three Rapid Tests for the Serodiagnosis of Hepatic Cystic Echinococcosis in Humans

et al. 2016

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BackgroundThe diagnosis of cystic echinococcosis (CE) is based primarily on imaging, in particular with ultrasound for abdominal CE, complemented by serology when imaging results are unclear. In rural endemic areas, where expertise in ultrasound may be scant and conventional serology techniques are unavailable due to lack of laboratory equipment, Rapid Diagnostic Tests (RDTs) are appealing.Methodology/Principal FindingsWe evaluated the diagnostic accuracy of 3 commercial RDTs for the diagnosis of hepatic CE. Sera from 59 patients with single hepatic CE cysts in well-defined ultrasound stages (gold standard) and 25 patients with non-parasitic cysts were analyzed by RDTs VIRapid HYDATIDOSIS (Vircell, Spain), Echinococcus DIGFA (Unibiotest, China), ADAMU-CE (ICST, Japan), and by RIDASCREEN Echinococcus IgG ELISA (R-Biopharm, Germany). Sensitivity, specificity and ROC curves were compared with McNemar and t-test. For VIRapid and DIGFA, correlation between semiquantitative results and ELISA OD values were evaluated by Spearman’s coefficient. Reproducibility was assessed on 16 randomly selected sera with Cohen’s Kappa coefficient. Sensitivity and Specificity of VIRapid (74%, 96%) and ADAMU-CE (57%, 100%) did not differ from ELISA (69%, 96%) while DIGFA (72%, 72%) did (p = 0.045). ADAMU-CE was significantly less sensitive in the diagnosis of active cysts (p = 0.019) while DIGFA was significantly less specific (p = 0.014) compared to ELISA. All tests were poorly sensitive in diagnosing inactive cysts (33.3% ELISA and ADAMU-CE, 42.8% DIGFA, 47.6% VIRapid). The reproducibility of all RDTs was good-very good. Band intensity of VIRapid and DIGFA correlated with ELISA OD values (r = 0.76 and r = 0.79 respectively, p<0.001).Conclusions/SignificanceRDTs may be useful in resource-poor settings to complement ultrasound diagnosis of CE in uncertain cases. VIRapid test appears to perform best among the examined kits, but all tests are poorly sensitive in the presence of inactive cysts, which may pose problems with accurate diagnosis.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Diagnostic Accuracy of Three Rapid Tests for the Serodiagnosis of Hepatic Cystic Echinococcosis in Humans

et al. 2016

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“…Additionally, antibodies from patients with other parasitic diseases including onchocerciasis, schistosomiasis, and toxocariasis have given rise to false positive reactions when tested against EgAgB [ 11 ]. It is worth mentioning the efforts of several authors to test new affordable and easy to use serological techniques instead of ELISA or the IB tests, making those immunoassays stand out based on particles such as DIGFA (dot immunogold filtration assay [ 43 , 44 ]; Table 2 ).…”

Section: Antigens Derived From Hydatid Fluidmentioning

confidence: 99%

“…The most frequently used crude extract of E. granulosus for the serodiagnosis of CE has been the somatic extract of protoscoleces (EgPpsSom). This extract has a Se ranging from 69.4% to 96.9% [ 11 , 16 , 43 , 44 , 52 – 54 ]. When compared with HF, the EgPpsSom performs worse.…”

Section: Antigens Derived From Hydatid Fluidmentioning

confidence: 99%

Serological Diagnosis and Follow-Up of Human Cystic Echinococcosis: A New Hope for the Future?

Manzano-Román

Sánchez-Ovejero

Hernández-González

et al. 2015

BioMed Research International

119

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Cystic echinococcosis (CE) is an important helminthic zoonotic disease caused by the Echinococcus granulosus complex. In humans, CE is a chronic disease driven by the growth of echinococcal cysts in different organs. Prognosis of this disease depends on multiple factors, including location, number, size, and stage of the cysts, making CE a disease of complex management. CE is usually asymptomatic for years and attracts limited attention from funding organizations and health authorities. For this reason, only experts' recommendations are available but no evidence-based conclusions have been drawn for CE clinical management. One of those pitfalls refers to the lack of evidence to support the use of serological tools for the diagnosis and follow-up of CE patients. In this respect, crude antigens are used to detect specific antibodies in patients, giving rise to false positive results. The advent of molecular techniques allowing the production of recombinant proteins has provided a number of candidate antigens that could overcome the problems associated with the use of crude parasite extracts in the serological assays. In this review, we present the last advances in this field, proposing the use of serology to support cyst stage-specific diagnosis and follow-up.

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“…Several recombinant antigens and synthetic peptides have been used for CE serodiagnosis in humans. [9][10][11][12][15][16][17][18] A highly antigenic protein, named EPC1, was characterized and isolated from a protoscolex (larval) stage and is encoded by the EPC1 gene. 13,17 Our previous study was aimed to assess the sequence of EPC1 isolated from different intermediate hosts of E. granulosus, including sheep (G1 strain) and camel (G6 strain).…”

Section: Discussionmentioning

confidence: 99%

A Study of Cross-Reactivity Between Recombinant EPC1 Antigen of Echinococcus granulosus in Serum from Patients with Confirmed Cystic Echinococcosis Infection and Other Parasitic Infections

Fathi¹,

Jalousian²,

Hosseini³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. A standardized test for the serodiagnosis of cystic echinococcosis (CE) remains an important challenge because of the problems in specificity and sensitivity of the available commercial kits and lack of proper evaluation of antigen. Using appropriate sources of antigenic material is crucial in improvement of the serological methods such as enzyme-linked immunosorbent assay (ELISA). This study was conducted to evaluate the performance of protein named Echinococcus protoscolex calcium binding protein EPC1 for the detection of antibodies in sera from patients with CE. Expressed and purified recombinant protein EPC1 (rEPC1) was used as antigen in ELISA method. Characterization of the rEPC1 antigen was evaluated using the serum of 25 patients with both surgical and imaging confirmed CE and 25 healthy donors as negative controls. Also, a panel of sera including chronic toxoplasmosis (IgG positive), strongyloidosis, fascioliasis, toxocariasis, and kala azar were used and patients with related parasites were confirmed by medical laboratories or clinically by research centers using microscopy or specific ELISA. rEPC1 showed relatively promising performance in total IgG ELISA for the detection of antibodies in sera from the negative controls, and the cut off value 0.4 units of optical density at 490 nm was calculated for ELISA. In this study, sensitivity of 100%, specificity of 93.7, positive predictive value of 92.6%, and negative predictive value of 100% were calculated for rEPC1. On the other hand, commercial ELISA kit based on the native antigen B of Echinococcus granulosus had sensitivity of 96.2% and specificity of 96.8%. No significant difference was found for sensitivity or specificity between the rEPC1 and commercial kit. However, rEPC1 may be a valuable antigen for diagnosis of human CE.

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The production and comparative evaluation of native and recombinant antigens for the fast serodiagnosis of cystic echinococcosis with dot immunogold filtration assay

Cited by 17 publications

References 11 publications

Comparison of the Diagnostic Accuracy of Three Rapid Tests for the Serodiagnosis of Hepatic Cystic Echinococcosis in Humans

Comparison of the Diagnostic Accuracy of Three Rapid Tests for the Serodiagnosis of Hepatic Cystic Echinococcosis in Humans

Serological Diagnosis and Follow-Up of Human Cystic Echinococcosis: A New Hope for the Future?

A Study of Cross-Reactivity Between Recombinant EPC1 Antigen of Echinococcus granulosus in Serum from Patients with Confirmed Cystic Echinococcosis Infection and Other Parasitic Infections

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