The process of lipid storage in insect oocytes: The involvement of β-chain of ATP synthase in lipophorin-mediated lipid transfer in the chagas’ disease vector Panstrongylus megistus (Hemiptera: Reduviidae)

Fruttero, Leonardo L.; Leyria, Jimena; Ramos, Fabian; Stariolo, Raúl; Settembrini, Beatriz P.; Canavoso, Lilián Etelvina

doi:10.1016/j.jinsphys.2016.10.014

Cited by 20 publications

(17 citation statements)

References 49 publications

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“…3 ). Although previously thought to only have a role in the mitochondria, ATPases are present in the plasma membrane of the insect midgut and salivary glands and have been shown to have a role in lipid transport 35 , 36 .…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

2018

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Increasing insecticide resistance in malaria-transmitting vectors represents a public health threat, but underlying mechanisms are poorly understood. Here, a data integration approach is used to analyse transcriptomic data from comparisons of insecticide resistant and susceptible Anopheles populations from disparate geographical regions across the African continent. An unbiased, integrated analysis of this data confirms previously described resistance candidates but also identifies multiple novel genes involving alternative resistance mechanisms, including sequestration, and transcription factors regulating multiple downstream effector genes, which are validated by gene silencing. The integrated datasets can be interrogated with a bespoke Shiny R script, deployed as an interactive web-based application, that maps the expression of resistance candidates and identifies co-regulated transcripts that may give clues to the function of novel resistance-associated genes.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

2018

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“…To analyze the histological distribution of β-ATPase and lipophorin, cryosections were sequentially incubated with an anti-β-ATPase antibody (dilution 1:100, rabbit anti-ATP5B/β-chain of ATP synthase of human origin, sc-33618, Santa Cruz Biotechnology, Inc., Santa Cruz, CA) followed by a goat anti-rabbit IgG labeled with Alexa 568 antibody (dilution 1:400, Molecular Probes, Eugene, OR) and a polyclonal anti-lipophorin-FITC antibody (dilution 1:40) obtained in our laboratory (Fruttero et al 2017a). Slides were mounted with Fluorsave (Calbiochem, Darmstadt, Germany) and observed with a Leica DMI 8 fluorescence microscope (Leica Microsystems, Wetzlar, Germany) or an Olympus FV300 laser scanning confocal microscope (Olympus, Tokyo, Japan), as corresponding.…”

Section: Methodsmentioning

confidence: 99%

“…The transmission of T. cruzi in endemic areas relies on the presence of hematophagous insects belonging to the subfamily Triatominae, commonly known as kissing bugs (Schofield et al 2006). Panstrongylus megistus (Burmeister), the main vector in Brazil after the control of Triatoma infestans (Klug) (Hemiptera: Reduviidae) populations (Coura 2015), has been employed as an experimental model to study the metabolism of lipids and lipoproteins mainly in the midgut and ovaries (Canavoso et al 2004; Fruttero et al 2009, 2011, 2014, 2017a). However, knowledge about the processes involved in lipid transfer to the fat body is still limited in this species.…”

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confidence: 99%

“…During this transfer process, the lipoprotein interacts with the plasma membrane of the target cells (Canavoso et al 2001, Van der Horst et al 2009). In this context, we have already demonstrated that the beta subunit of the F 1 F O adenosine triphosphate synthase complex (β-ATPase), located on the plasma membrane of the cells, acts as a docking receptor of lipophorin in the midgut and in the ovaries of P. megistus (Fruttero et al 2014, 2017a). The ATP synthase complex is located in the inner mitochondrial membrane, where it synthesizes ATP at the expense of the transmembrane electrochemical proton potential difference (Walker 1998).…”

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confidence: 99%

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The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

Fruttero

Leyria

Moyetta

et al. 2019

Journal of Insect Science

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In insects, lipid transfer to the tissues is mediated by lipophorin, the major circulating lipoprotein, mainly through a nonendocytic pathway involving docking receptors. Currently, the role of such receptors in lipid metabolism remains poorly understood. In this work, we performed a histological characterization of the fat body of the Chagas’ disease vector, Panstrongylus megistus (Burmeister), subjected to different nutritional conditions. In addition, we addressed the role of the β-chain of ATP synthase (β-ATPase) in the process of lipid transfer from lipophorin to the fat body. Fifth-instar nymphs in either fasting or fed condition were employed in the assays. Histological examination revealed that the fat body was composed by diverse trophocyte phenotypes. In the fasting condition, the cells were smaller and presented a homogeneous cytoplasmic content. The fat body of fed insects increased in size mainly due to the enlargement of lipid stores. In this condition, trophocytes contained abundant lipid droplets, and the rough endoplasmic reticulum was highly developed and mitochondria appeared elongated. Immunofluorescence assays showed that the β-ATPase, a putative lipophorin receptor, was located on the surface of fat body cells colocalizing partially with lipophorin, which suggests their interaction. No changes in β-ATPase expression were found in fasting and fed insects. Blocking the lipophorin–β-ATPase interaction impaired the lipophorin-mediated lipid transfer to the fat body. The results showed that the nutritional status of the insect influenced the morphohistological features of the tissue. Besides, these findings suggest that β-ATPase functions as a lipophorin docking receptor in the fat body.

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“…Sevala et al 1997Sevala et al , (1999 demonstrated in Blattella germanica revealed that lipophorins also play a role as juvenile hormone (JH) binding protein and suggests that it may contribute to the regulation of JH titers in the hemolymph. It was also suggested that in case of two triatomine species, lipophorins play a role as yolk protein, being endocytosed by the oocytes, stored in yolk bodies and used to support embryonic development (Kawooya and Law, 1988;Ziegler and Van Antwerpen, 2006;Fruttero et al, 2011Fruttero et al, , 2017Leyria et al, 2014).…”

Section: Lipid Transporting Functionmentioning

confidence: 99%

A Comparative Protein Profile of Virgin and Mated Male of Leucinodes Orbonalis Accessory Gland

Saraswathi¹,

B.S²,

Kiran³

et al. 2020

Preprint

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Male accessory gland (MAG) proteins that are secretory in nature delivered to females, along with sperms affects female reproductive physiology and behavior. In our study, proteomic approaches were employed to identify the MAG proteins of L. orbonalis, a monophagous and most destructive pest on brinjal. A set of 117 spots in virgin MAG and 186 in mated MAG were obtained from 2-D gel electrophoresis. The differentially expressed MAG proteins after mating in comparison with the virgin male were 14 upregulated and 16 down-regulated. We have used MALDI- MS to identify the 13 unique proteins within the virgin MAG of the L. orbonalis and analyzed with the Swiss-Prot database using a Mascot search engine. The proteins were identified as proteolysis regulators, lipid transporter, olfactory protein, metabolism, DNA binding, and hexamerins. This is the first report on proteome analysis of MAG of the Leucinodes orbonalis.

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The process of lipid storage in insect oocytes: The involvement of β-chain of ATP synthase in lipophorin-mediated lipid transfer in the chagas’ disease vector Panstrongylus megistus (Hemiptera: Reduviidae)

Cited by 20 publications

References 49 publications

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

Transcriptomic meta-signatures identified in Anopheles gambiae populations reveal previously undetected insecticide resistance mechanisms

The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer

A Comparative Protein Profile of Virgin and Mated Male of Leucinodes Orbonalis Accessory Gland

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